LOCUS       sequence-401803-       10474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  TITLE     Alarcon lab plasmids
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 LARP7-FLAG 7SK
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2890..3138
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3061..3080
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3725..3842
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3891..4401
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4320..4338
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6204..6233
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6252..6275
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6309..6882
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6476..6493)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6703..6722
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6902..7927
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6902..7927
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7951..7967)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7952..7968)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8050..8283
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8355..8476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8392..8411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8446..8465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8516..8651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8671..8690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8672..8690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8700..8717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8700..8716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8709..8731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8858..9313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8945..8964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9155..9176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9339..9443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9444..9512,9513..10304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9662..9681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     3121 tggaaaggac gaaacaccgg gatgtgaggg cgatctggct gcgacatctg tcaccccatt
     3181 gatcgccagg gttgattcgg ctgatctggc tggctaggcg ggtgtcccct tcctccctca
     3241 ccgctccatg tgcgtccctc ccgaagctgc gcgctcggtc gaagaggacg accatccccg
     3301 atagaggagg accggtcttc ggtcaagggt atacgagtag ctgcgctccc ctgctagaac
     3361 ctccaaacaa gctctcaagg tccatttgta ggagaacgta gggtagtcaa gcttccaaga
     3421 ctccagacac atccaaatga ggcgctgcat gtggcagtct gcctttcttt tgacccatta
     3481 cccatctaag ttagatgctt ttttaaatgt tttttaattt ttaaattttt aatttttttc
     3541 attatttatt ttttattttt gagacggatc tcggctcact gtaacctcca cctcccgggt
     3601 tcaagcgatt ctcctgactc agcctcccga gtactgggat tacaggcgcg cgccaccatg
     3661 ccggtccgca ggtatgcacg cgtgaattct cgacctcgag acaaatggca gtattcatcc
     3721 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     3781 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     3841 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     3901 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     3961 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     4021 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     4081 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     4141 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     4201 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     4261 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     4321 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     4381 atcaccgacc tctctcccca gggggatccg gtaccgagga gatctgccgc cgcgatcgca
     4441 tggaaactga aagtggaaat caggaaaagg taatggaaga agaaagcact gaaaagaaaa
     4501 aagaagttga aaaaaagaaa cggtcacgag ttaaacaggt gcttgcagat attgctaagc
     4561 aagtggactt ctggtttggg gatgcaaatc ttcacaagga tagatttctt cgagaacaga
     4621 tagaaaaatc tagagatgga tatgttgata tatcactact tgtgtctttt aacaaaatga
     4681 aaaaattgac tactgatggg aagttaattg ccagagcgtt gagaagttca gctgttgtag
     4741 agcttgattt ggaaggcacc agaatccgga ggaaaaaacc tctgggggaa agaccaaagg
     4801 atgaggatga acgcacagtg tatgtggagt tacttcccaa aaatgttaat cacagctgga
     4861 ttgaaagagt atttgggaaa tgtggcaatg ttgtttatat aagtatacca cattataagt
     4921 ctactggaga tccaaaggga tttgcgtttg tggaatttga aacaaaagaa caagcagcaa
     4981 aagcaattga gtttcttaac aacccaccag aagaagcacc aagaaaacct ggcatatttc
     5041 ctaaaacagt gaaaaataag cccattccag ccttaagagt tgtggaagag aagaaaaaga
     5101 aaaagaagaa gaaaggccga atgaaaaagg aagacaatat ccaagccaaa gaagaaaaca
     5161 tggacacaag caacaccagc atcagtaaaa tgaaaagatc cagacccaca tctgagggct
     5221 ctgacattga gtccactgaa ccccaaaagc agtgctcaaa gaaaaagaaa aaacgggaca
     5281 gagttgaagc atctagctta cctgaagtca gaacagggaa gaggaagaga agcagctctg
     5341 aagatgcaga atccctagct ccccgatcaa aagtaaagaa aattattcag aaagacatca
     5401 ttaaggaagc atcagaagct tccaaggaaa atagagatat agaaatctct actgaagagg
     5461 aaaaggatac tggagatcta aaagatagct ctctcttgaa aacaaaaagg aaacataaga
     5521 aaaaacataa agagagacat aaaatgggag aagaagttat accattaaga gtgctatcaa
     5581 agagcgaatg gatggatttg aaaaaagagt atttagcgct acaaaaagct agcatggctt
     5641 ctttaaaaaa aacaatatcc caaataaaat cagagtcaga aatggaaaca gacagtggag
     5701 tacctcaaaa cactggaatg aaaaatgaaa aaacagccaa cagggaagag tgtcgcaccc
     5761 aggagaaagt taatgcaaca ggaccacagt tcgtgagtgg agtgattgtg aagatcatta
     5821 gcacagagcc tctacctggc aggaaacaag tccgggatac tttggcagca atctcagaag
     5881 ttctttatgt tgatttgcta gaaggggata cagaatgcca tgctagattt aaaactcctg
     5941 aggatgctca agcagtaata aatgcctata cagaaattaa caagaaacac tgctggaaac
     6001 tcgagatcct ttctggtgat cacgaacaaa ggtattggca gaagattttg gttgatagac
     6061 aggcaaaact taatcagcct cgggaaaaga aaagaggcac tgaaaagtta atcaccaaag
     6121 ctgaaaagat taggctggca aagactcaac aagcgagtaa acatataaga ttttctgaat
     6181 atgatacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     6241 atgatatcct ggattacaag gatgacgacg ataaggttta aacctaggcg tagcggccgc
     6301 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     6361 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     6421 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     6481 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     6541 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     6601 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     6661 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     6721 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     6781 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6841 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatcccgggg ggcaatgaga
     6901 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     6961 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     7021 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     7081 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     7141 tggggaattt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     7201 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     7261 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     7321 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     7381 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     7441 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     7501 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     7561 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     7621 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     7681 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     7741 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     7801 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     7861 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     7921 ggaatagagt agatgccgac cgggatctat cgataccgtc gacctcgagg gggggcccgg
     7981 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8041 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8101 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8161 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8221 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8281 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8341 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8461 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     8521 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8581 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8641 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8701 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8761 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8821 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9061 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9181 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     9301 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     9361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9661 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10021 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10081 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    10321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10441 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
