LOCUS       sequence-401921-        2599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 55209 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-acc2
FEATURES             Location/Qualifiers
     source          1..2599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(734..1322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(814..833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1413..2204)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctcagg agcacagttc
       61 aagtctcgcg cgtgacgtgt gtttcatagt gtctcattta gtgtggtgtt gggttgtcgt
      121 gtctcacctg gtccccttgc tcacagtccc caaaaaaccg tccaaattgt cgacaatcgt
      181 atcatttttt gccggtgcgt acgaaactca ccagtctccc gtggtccttt cgttttggaa
      241 caaggggatt attccttcac tctgtgtgtg tgtgtgactg tcccttgtcg catccctctc
      301 ttttctcgcc gtcactgact gtgagtaacg tctccatatc gacccagtcc gttcgttggt
      361 tggactcgcg agccccgcgc actggagtca gggtacgtac gtcccgacaa aatatccttg
      421 acttcctccc acgcgtcgcg atgcgatgcg tcccatcaca gcctttcttg accctcgttt
      481 tcaatcactc accaacactg acagtgatac tcttttccct agtgccactc ttctcctcgt
      541 gacactcgtt actagcaacg caacaacaac agctcgatcg attgtggcgt caatgcgaga
      601 ccaccaggat acatagatta ccacaactcc gagcccttcc acccacagaa tcaggggata
      661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1381 aagggatttt ggtcatgcat tctaggtgat tattatttgc cgactacctt ggtgatctcg
     1441 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1501 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     1561 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     1621 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     1681 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     1741 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     1801 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agataccagc aagaatgtca
     1861 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     1921 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcactctc tccaggggaa
     1981 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     2041 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     2101 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     2161 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgcgaaa cgatcctcat
     2221 cctgtctctt gatcagatat tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     2281 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     2341 ggttcgcttg ctaagcttgc atgcctgcaa gtcgactcta gaggagcggt gatcacaggc
     2401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
     2461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
     2521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
     2581 ggtgattttg tgccgagct
//
