LOCUS       sequence-402478-       10966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector for the expression of the 
            Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary 
            translation initiation site for H2B-mCherry expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Shu T et al.
  TITLE     nucGEMs probe the biophysical properties of the nucleoplasm
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pUBC-Pfv-Sapphire-IRES-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..10966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    665..790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1287..1520
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1705..1749
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2012..2127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2173..3384
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3221..3240
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5211..5783
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5378..5395)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5799..6176
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      6189..6198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6195..6905
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6340..6358)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6608..6627
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6919..7507
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6972..6992)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7390..7401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7714..8347
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8475..8491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8475..8491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8488..8510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8499..8515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8523..8529,8530..8547,8548..8553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8568..8589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8706..8723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8877..9465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8957..8976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9636..10427,10428..10496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10259..10278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10497..10601)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10649..10783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10686..10705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10740..10759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10951..10966,1..618)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
       61 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
      121 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
      181 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
      241 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
      301 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
      361 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
      421 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      601 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      661 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      721 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      781 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      841 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      901 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      961 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1021 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1081 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     1141 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1201 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1261 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1321 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1381 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1441 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1501 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1561 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1621 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1681 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1741 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1801 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     1861 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     1921 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     1981 cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta
     2041 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2101 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2161 ttatttaatt aaggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     2221 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     2281 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     2341 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     2401 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     2461 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     2521 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     2581 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     2641 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     2701 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     2761 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     2821 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     2881 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     2941 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     3001 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     3061 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     3121 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     3181 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     3241 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     3301 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     3361 ccgtttttgg cttttttgtt agacgaattc gccaccatgt ctagactctc tatcaacccg
     3421 acattgataa accgggacaa accgtatact aaagaagaac ttatggaaat cctgcggctg
     3481 gcaatcattg ctgagttgga cgcgatcaac ctctatgaac agatggcacg gtattctgaa
     3541 gatgagaacg taaggaagat tctccttgac gtcgctcggg aggagaaggc gcacgtgggt
     3601 gagtttatgg cactccttct taaccttgac cctgagcaag tgactgagtt gaaaggcggc
     3661 ttcgaagagg taaaagaact gacaggcatt gaagcccata ttaacgataa taaaaaagaa
     3721 gagtccaacg tcgaatattt tgaaaaactc cgctccgctc tgttggatgg agtaaataag
     3781 gggaggagtc tccttaagca tctgccagtt acacgcatag aagggcagtc ctttcgagta
     3841 gacattatta aatttgagga tggggtgcgc gtcgttaaac aggaatacaa gcccattcca
     3901 cttctcaaaa agaaatttta cgtaggaatt agagaactga atgatggtac gtacgacgtt
     3961 tctattgcca cgaaggcagg tgaattgttg gttaaggacg aggaaagcct tgtgataaga
     4021 gaaatcctgt caactgaagg gataaagaaa atgaagttga gtagctggga caatcccgag
     4081 gaggcgctga atgatcttat gaatgccctt caggaagcct ctaatgcttc cgctggacct
     4141 ttcggtctga taattaatcc aaagagatac gcgaaacttc ttaaaatcta tgagaaatca
     4201 ggaaaaatgt tggtagaagt gctgaaagaa atttttcggg gtggcatcat agtcacactg
     4261 aacatagatg aaaacaaggt tattattttt gcaaatactc cagcggtgct tgacgtagtt
     4321 gttgggcagg acgtaacgct ccaggaattg ggacctgagg gggacgacgt ggcctttttg
     4381 gtgtcagaag ccatcggcat ccgaataaag aaccccgaag cgattgttgt cctcgaatgg
     4441 tcagggtcag gttctggctc aggtagcggg agtgggcggc cgctgatgtc taagggggag
     4501 gagcttttca caggtgtagt acctattctt gttgaattgg atggcgatgt caatggacac
     4561 aaattctccg tctcagggga gggagaggga gacgctacct atggaaagtt gactctcaaa
     4621 ttcatctgca caacgggtaa gttgccggtc ccttggccga cactcgttac tacgttcagc
     4681 tatggagtac agtgtttcgc gcgctacccc gaccatatga aacaacacga ctttttcaaa
     4741 tccgcaatgc ccgagggata cgttcaggaa cggactattt tctttaaaga cgacggtaat
     4801 tacaaaacac gcgccgaagt taagttcgaa ggagatactt tggttaaccg aatagagctg
     4861 aaaggcattg atttcaaaga ggacgggaac atcctgggtc ataaacttga gtataatttt
     4921 aattcccaca acgtgtatat aatggcagat aaacagaaga atggcatcaa ggttaacttc
     4981 aagattcggc acaatattga agacggtagt gtacagctgg ctgaccacta tcagcagaat
     5041 acgccaattg gtgatgggcc cgtcttgttg ccagataatc actacctctc aatccagagt
     5101 gcccttagta aagatccaaa cgaaaagcgg gaccacatgg ttttgttgga gtttgtcaca
     5161 gctgctggga ttacccacgg gatagacgag ttgtataaat gactcgagcg cccctctccc
     5221 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5281 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5341 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5401 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5461 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5521 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5581 tggatagttg tggaaagagt caaatggctc acctcaagcg tattcaacaa ggggctgaag
     5641 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5701 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5761 tcctttgaaa aacacgatga taagcctgca gggccaccat gcctgaaccc tctaagtctg
     5821 ctccagcccc taaaaagggt tctaagaagg ctatcactaa ggcgcagaag aaggatggta
     5881 agaagcgtaa gcgcagccgc aaggagagct attctatcta tgtgtacaag gttctgaagc
     5941 aggtccaccc cgacaccggc atctcatcca aggccatggg gatcatgaac tccttcgtca
     6001 acgacatctt cgagcgcatc gcgggcgagg cttctcgcct ggctcactac aataagcgct
     6061 cgaccatcac ctccagggag attcagacgg ctgtgcgcct gctgctgcct ggggagctgg
     6121 ctaagcatgc tgtgtccgag ggcactaagg cagttaccaa gtacactagc tctaaggatc
     6181 caccagtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     6241 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     6301 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     6361 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     6421 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     6481 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     6541 cctccctgca agacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     6601 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     6661 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     6721 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     6781 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     6841 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     6901 agtaaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6961 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7021 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7081 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7141 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7201 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7261 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7321 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7381 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7441 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7501 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     7561 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7621 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7681 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     7741 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     7801 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     7861 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     7921 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     7981 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     8041 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     8101 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     8161 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8221 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8281 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8341 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8401 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     8461 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8521 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8581 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8641 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8701 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8761 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8881 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9241 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9301 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9361 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10201 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10261 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10621 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    10681 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10741 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10801 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    10861 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    10921 ttgttaaata tgtactacaa acttagtagt tggaagggct aattca
//
