LOCUS       sequence-402481-       10407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector for the expression of the 
            Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin 
            selection marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Shu T et al.
  TITLE     nucGEMs probe the biophysical properties of the nucleoplasm
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pUBC-Pfv-Sapphire_PURO
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             10..643
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    690..815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1312..1545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1730..1774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2037..2152
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2198..3409
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3246..3265
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        5253..5752
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5288..5311)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5685..5704
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5773..6372
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5773..5792)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6269..6289
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6385..6973
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6438..6458)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6856..6867)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7180..7813
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7941..7957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7941..7957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7954..7976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7965..7981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7989..7995,7996..8013,8014..8019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8034..8055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8172..8189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8343..8931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8423..8442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9102..9893,9894..9962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9725..9744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9963..10067)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10115..10249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10152..10171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10206..10225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
       61 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
      121 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
      181 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
      241 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
      301 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
      361 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
      421 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1201 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1261 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1321 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1381 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1441 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1501 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1561 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1621 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     1681 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     1741 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     1801 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     1861 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     1921 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     1981 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     2041 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2101 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2161 attacaggga cagcagagat ccagtttatt taattaaggc ctccgcgccg ggttttggcg
     2221 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     2281 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     2341 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     2401 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     2461 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     2521 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     2581 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     2641 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     2701 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     2761 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     2821 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     2881 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     2941 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     3001 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     3061 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     3121 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     3181 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     3241 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     3301 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     3361 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aattcgccac
     3421 catgtctaga ctctctatca acccgacatt gataaaccgg gacaaaccgt atactaaaga
     3481 agaacttatg gaaatcctgc ggctggcaat cattgctgag ttggacgcga tcaacctcta
     3541 tgaacagatg gcacggtatt ctgaagatga gaacgtaagg aagattctcc ttgacgtcgc
     3601 tcgggaggag aaggcgcacg tgggtgagtt tatggcactc cttcttaacc ttgaccctga
     3661 gcaagtgact gagttgaaag gcggcttcga agaggtaaaa gaactgacag gcattgaagc
     3721 ccatattaac gataataaaa aagaagagtc caacgtcgaa tattttgaaa aactccgctc
     3781 cgctctgttg gatggagtaa ataaggggag gagtctcctt aagcatctgc cagttacacg
     3841 catagaaggg cagtcctttc gagtagacat tattaaattt gaggatgggg tgcgcgtcgt
     3901 taaacaggaa tacaagccca ttccacttct caaaaagaaa ttttacgtag gaattagaga
     3961 actgaatgat ggtacgtacg acgtttctat tgccacgaag gcaggtgaat tgttggttaa
     4021 ggacgaggaa agccttgtga taagagaaat cctgtcaact gaagggataa agaaaatgaa
     4081 gttgagtagc tgggacaatc ccgaggaggc gctgaatgat cttatgaatg cccttcagga
     4141 agcctctaat gcttccgctg gacctttcgg tctgataatt aatccaaaga gatacgcgaa
     4201 acttcttaaa atctatgaga aatcaggaaa aatgttggta gaagtgctga aagaaatttt
     4261 tcggggtggc atcatagtca cactgaacat agatgaaaac aaggttatta tttttgcaaa
     4321 tactccagcg gtgcttgacg tagttgttgg gcaggacgta acgctccagg aattgggacc
     4381 tgagggggac gacgtggcct ttttggtgtc agaagccatc ggcatccgaa taaagaaccc
     4441 cgaagcgatt gttgtcctcg aatggtcagg gtcaggttct ggctcaggta gcgggagtgg
     4501 gcggccgctg atgtctaagg gggaggagct tttcacaggt gtagtaccta ttcttgttga
     4561 attggatggc gatgtcaatg gacacaaatt ctccgtctca ggggagggag agggagacgc
     4621 tacctatgga aagttgactc tcaaattcat ctgcacaacg ggtaagttgc cggtcccttg
     4681 gccgacactc gttactacgt tcagctatgg agtacagtgt ttcgcgcgct accccgacca
     4741 tatgaaacaa cacgactttt tcaaatccgc aatgcccgag ggatacgttc aggaacggac
     4801 tattttcttt aaagacgacg gtaattacaa aacacgcgcc gaagttaagt tcgaaggaga
     4861 tactttggtt aaccgaatag agctgaaagg cattgatttc aaagaggacg ggaacatcct
     4921 gggtcataaa cttgagtata attttaattc ccacaacgtg tatataatgg cagataaaca
     4981 gaagaatggc atcaaggtta acttcaagat tcggcacaat attgaagacg gtagtgtaca
     5041 gctggctgac cactatcagc agaatacgcc aattggtgat gggcccgtct tgttgccaga
     5101 taatcactac ctctcaatcc agagtgccct tagtaaagat ccaaacgaaa agcgggacca
     5161 catggttttg ttggagtttg tcacagctgc tgggattacc cacgggatag acgagttgta
     5221 taaatgactc gatagcctgc aggaattcta ccgggtaggg gaggcgcttt tcccaaggca
     5281 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     5341 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     5401 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     5461 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5521 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     5581 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     5641 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5701 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     5761 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5821 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5881 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5941 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6001 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6061 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6121 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6181 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6241 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6301 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6361 cccggtgcct gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     6421 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6481 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6541 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6601 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6661 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6721 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     6781 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6841 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6901 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6961 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     7021 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7081 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7141 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7201 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7261 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7321 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7381 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     7441 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     7501 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     7561 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     7621 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7801 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7861 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     7921 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7981 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8101 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10081 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    10141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10261 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10321 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    10381 ttgtatttgt taaatatgta ctacaaa
//
