LOCUS       sequence-402483-       10993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector for the expression of the 
            Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC 
            promoter, with a secondary translation initiation site for 
            H2B-mCherry expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Shu T et al.
  TITLE     nucGEMs probe the biophysical properties of the nucleoplasm
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..10993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2189..3400
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3237..3256
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3422..3442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5254..5826
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5421..5438)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5842..6219
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      6232..6241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6238..6948
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6383..6401)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6651..6670
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6962..7550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7015..7035)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7433..7444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7757..8390
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8518..8534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8518..8534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8531..8553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8542..8558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8566..8572,8573..8590,8591..8596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8611..8632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8749..8766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8920..9508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9000..9019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9679..10470,10471..10539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10302..10321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10540..10644)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10692..10826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10729..10748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10783..10802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat ttaattaagg cctccgcgcc gggttttggc gcctcccgcg
     2221 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     2281 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     2341 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     2401 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     2461 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     2521 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     2581 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     2641 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     2701 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     2761 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     2821 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     2881 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     2941 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     3001 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     3061 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     3121 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     3181 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     3241 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     3301 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     3361 gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaattcgcca ccatgtctag
     3421 accaaaaaag aagagaaagg tctctagact ctctatcaac ccgacattga taaaccggga
     3481 caaaccgtat actaaagaag aacttatgga aatcctgcgg ctggcaatca ttgctgagtt
     3541 ggacgcgatc aacctctatg aacagatggc acggtattct gaagatgaga acgtaaggaa
     3601 gattctcctt gacgtcgctc gggaggagaa ggcgcacgtg ggtgagttta tggcactcct
     3661 tcttaacctt gaccctgagc aagtgactga gttgaaaggc ggcttcgaag aggtaaaaga
     3721 actgacaggc attgaagccc atattaacga taataaaaaa gaagagtcca acgtcgaata
     3781 ttttgaaaaa ctccgctccg ctctgttgga tggagtaaat aaggggagga gtctccttaa
     3841 gcatctgcca gttacacgca tagaagggca gtcctttcga gtagacatta ttaaatttga
     3901 ggatggggtg cgcgtcgtta aacaggaata caagcccatt ccacttctca aaaagaaatt
     3961 ttacgtagga attagagaac tgaatgatgg tacgtacgac gtttctattg ccacgaaggc
     4021 aggtgaattg ttggttaagg acgaggaaag ccttgtgata agagaaatcc tgtcaactga
     4081 agggataaag aaaatgaagt tgagtagctg ggacaatccc gaggaggcgc tgaatgatct
     4141 tatgaatgcc cttcaggaag cctctaatgc ttccgctgga cctttcggtc tgataattaa
     4201 tccaaagaga tacgcgaaac ttcttaaaat ctatgagaaa tcaggaaaaa tgttggtaga
     4261 agtgctgaaa gaaatttttc ggggtggcat catagtcaca ctgaacatag atgaaaacaa
     4321 ggttattatt tttgcaaata ctccagcggt gcttgacgta gttgttgggc aggacgtaac
     4381 gctccaggaa ttgggacctg agggggacga cgtggccttt ttggtgtcag aagccatcgg
     4441 catccgaata aagaaccccg aagcgattgt tgtcctcgaa tggtcagggt caggttctgg
     4501 ctcaggtagc gggagtgggc ggccgctgat gtctaagggg gaggagcttt tcacaggtgt
     4561 agtacctatt cttgttgaat tggatggcga tgtcaatgga cacaaattct ccgtctcagg
     4621 ggagggagag ggagacgcta cctatggaaa gttgactctc aaattcatct gcacaacggg
     4681 taagttgccg gtcccttggc cgacactcgt tactacgttc agctatggag tacagtgttt
     4741 cgcgcgctac cccgaccata tgaaacaaca cgactttttc aaatccgcaa tgcccgaggg
     4801 atacgttcag gaacggacta ttttctttaa agacgacggt aattacaaaa cacgcgccga
     4861 agttaagttc gaaggagata ctttggttaa ccgaatagag ctgaaaggca ttgatttcaa
     4921 agaggacggg aacatcctgg gtcataaact tgagtataat tttaattccc acaacgtgta
     4981 tataatggca gataaacaga agaatggcat caaggttaac ttcaagattc ggcacaatat
     5041 tgaagacggt agtgtacagc tggctgacca ctatcagcag aatacgccaa ttggtgatgg
     5101 gcccgtcttg ttgccagata atcactacct ctcaatccag agtgccctta gtaaagatcc
     5161 aaacgaaaag cgggaccaca tggttttgtt ggagtttgtc acagctgctg ggattaccca
     5221 cgggatagac gagttgtata aatgactcga gcgcccctct ccctcccccc cccctaacgt
     5281 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5341 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5401 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5461 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5521 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5581 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5641 agtcaaatgg ctcacctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5701 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5761 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     5821 tgataagcct gcagggccac catgcctgaa ccctctaagt ctgctccagc ccctaaaaag
     5881 ggttctaaga aggctatcac taaggcgcag aagaaggatg gtaagaagcg taagcgcagc
     5941 cgcaaggaga gctattctat ctatgtgtac aaggttctga agcaggtcca ccccgacacc
     6001 ggcatctcat ccaaggccat ggggatcatg aactccttcg tcaacgacat cttcgagcgc
     6061 atcgcgggcg aggcttctcg cctggctcac tacaataagc gctcgaccat cacctccagg
     6121 gagattcaga cggctgtgcg cctgctgctg cctggggagc tggctaagca tgctgtgtcc
     6181 gagggcacta aggcagttac caagtacact agctctaagg atccaccagt cgccaccatg
     6241 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     6301 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     6361 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     6421 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     6481 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     6541 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaagacggc
     6601 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     6661 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     6721 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     6781 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     6841 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     6901 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaac gcgtctggaa
     6961 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7021 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7081 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7141 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7201 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7261 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7321 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7381 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7441 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7501 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     7561 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7621 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7681 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7741 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     7801 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7861 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7921 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7981 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     8041 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     8101 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     8161 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     8221 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8281 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8341 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8401 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8461 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10681 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10801 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10861 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    10921 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10981 caaacttagt agt
//
