LOCUS       sequence-402486-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector for the expression of the 
            Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC 
            promoter, with a puromycin selection marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Shu T et al.
  TITLE     nucGEMs probe the biophysical properties of the nucleoplasm
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pUBC-SV40NLS-Pfv-Sapphire-PURO
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2189..3400
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3237..3256
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3422..3442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        5271..5770
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5306..5329)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5703..5722
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5791..6390
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5791..5810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6287..6307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6403..6991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6456..6476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6874..6885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7198..7831
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7959..7975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7959..7975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7972..7994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7983..7999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8007..8013,8014..8031,8032..8037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8052..8073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8190..8207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8361..8949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8441..8460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9120..9911,9912..9980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9743..9762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9981..10085)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10133..10267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10170..10189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10224..10243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat ttaattaagg cctccgcgcc gggttttggc gcctcccgcg
     2221 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     2281 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     2341 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     2401 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     2461 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     2521 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     2581 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     2641 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     2701 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     2761 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     2821 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     2881 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     2941 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     3001 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     3061 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     3121 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     3181 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     3241 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     3301 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     3361 gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaattcgcca ccatgtctag
     3421 accaaaaaag aagagaaagg tctctagact ctctatcaac ccgacattga taaaccggga
     3481 caaaccgtat actaaagaag aacttatgga aatcctgcgg ctggcaatca ttgctgagtt
     3541 ggacgcgatc aacctctatg aacagatggc acggtattct gaagatgaga acgtaaggaa
     3601 gattctcctt gacgtcgctc gggaggagaa ggcgcacgtg ggtgagttta tggcactcct
     3661 tcttaacctt gaccctgagc aagtgactga gttgaaaggc ggcttcgaag aggtaaaaga
     3721 actgacaggc attgaagccc atattaacga taataaaaaa gaagagtcca acgtcgaata
     3781 ttttgaaaaa ctccgctccg ctctgttgga tggagtaaat aaggggagga gtctccttaa
     3841 gcatctgcca gttacacgca tagaagggca gtcctttcga gtagacatta ttaaatttga
     3901 ggatggggtg cgcgtcgtta aacaggaata caagcccatt ccacttctca aaaagaaatt
     3961 ttacgtagga attagagaac tgaatgatgg tacgtacgac gtttctattg ccacgaaggc
     4021 aggtgaattg ttggttaagg acgaggaaag ccttgtgata agagaaatcc tgtcaactga
     4081 agggataaag aaaatgaagt tgagtagctg ggacaatccc gaggaggcgc tgaatgatct
     4141 tatgaatgcc cttcaggaag cctctaatgc ttccgctgga cctttcggtc tgataattaa
     4201 tccaaagaga tacgcgaaac ttcttaaaat ctatgagaaa tcaggaaaaa tgttggtaga
     4261 agtgctgaaa gaaatttttc ggggtggcat catagtcaca ctgaacatag atgaaaacaa
     4321 ggttattatt tttgcaaata ctccagcggt gcttgacgta gttgttgggc aggacgtaac
     4381 gctccaggaa ttgggacctg agggggacga cgtggccttt ttggtgtcag aagccatcgg
     4441 catccgaata aagaaccccg aagcgattgt tgtcctcgaa tggtcagggt caggttctgg
     4501 ctcaggtagc gggagtgggc ggccgctgat gtctaagggg gaggagcttt tcacaggtgt
     4561 agtacctatt cttgttgaat tggatggcga tgtcaatgga cacaaattct ccgtctcagg
     4621 ggagggagag ggagacgcta cctatggaaa gttgactctc aaattcatct gcacaacggg
     4681 taagttgccg gtcccttggc cgacactcgt tactacgttc agctatggag tacagtgttt
     4741 cgcgcgctac cccgaccata tgaaacaaca cgactttttc aaatccgcaa tgcccgaggg
     4801 atacgttcag gaacggacta ttttctttaa agacgacggt aattacaaaa cacgcgccga
     4861 agttaagttc gaaggagata ctttggttaa ccgaatagag ctgaaaggca ttgatttcaa
     4921 agaggacggg aacatcctgg gtcataaact tgagtataat tttaattccc acaacgtgta
     4981 tataatggca gataaacaga agaatggcat caaggttaac ttcaagattc ggcacaatat
     5041 tgaagacggt agtgtacagc tggctgacca ctatcagcag aatacgccaa ttggtgatgg
     5101 gcccgtcttg ttgccagata atcactacct ctcaatccag agtgccctta gtaaagatcc
     5161 aaacgaaaag cgggaccaca tggttttgtt ggagtttgtc acagctgctg ggattaccca
     5221 cgggatagac gagttgtata aatgactcga tagcctgcag gaattctacc gggtagggga
     5281 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     5341 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     5401 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     5461 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5521 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     5581 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     5641 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     5701 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5761 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     5821 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5881 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5941 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6001 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6061 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6121 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6181 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6241 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6301 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6361 tggtgcatga cccgcaagcc cggtgcctga cgcgtctgga acaatcaacc tctggattac
     6421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6781 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6961 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     7021 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     7081 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     7141 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     7201 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     7261 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7321 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7381 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7441 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7501 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7561 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7621 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7681 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7741 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7801 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7861 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     7921 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7981 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8041 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8101 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8161 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8221 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8281 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8581 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8761 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9001 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9421 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9721 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10081 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
    10141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10261 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10321 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10381 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
