LOCUS       sequence-402493-        3889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jAspSnFR3 in Gateway cloning entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
  TITLE     An engineered biosensor enables dynamic aspartate measurements in 
            living cells.
  JOURNAL   eLife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024.
  PUBMED    38393319
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-02-23"; volume: "12:RP90024. doi"; pages: " 
            10.7554/eLife.90024"
COMMENT     Sequence Label: pENTR1a-jAspSnFR3
FEATURES             Location/Qualifiers
     source          1..3889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     1490..1511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1614..1635)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(2118..2217)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2329..2348)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2340..3149
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2407..2426)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3239..3827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3728..3747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgaattcg cttactaaaa gccagaccac catgggatcc gccgcaggca
      541 gcacgctgga caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt aaatctgccg
      601 tgcctttctc ttattacgac agtcagcaca aagtggtggg ttactcgcag gaatactcca
      661 acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag gtaaaactga
      721 ttccgattac cccacaaaac cgtattccac tgctgcaaaa cggcacttac gattttgaat
      781 gtggttctac caccaacaac gtcgaacgcc aaaaacaggc ggctttctct gacactattt
      841 tcgtggtcgg tacgcgcctg ttgaccaaaa agggtggcga tatcaaagat tttgccaacc
      901 tgaaagacaa agccgtagtc gtcacttccg gcactacctc tgaagttttg ctcaacaaac
      961 tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac ggtgactctt
     1021 tccgcaccct ggaaagcggt cgtgccgttg cctttatgat ggatgacgtg ctgctggccg
     1081 gtgaacgtgc gaaagcgaag aaaccagacg actgggaaat cgtcggcaag ccgcagtctc
     1141 aggaggccta cggttgtatg ttgcgtaaag atgatccgca gttcaaaaag ctgatggatg
     1201 acaccatcgc tcaggtgcag acctccggtg aagcggaaaa atggtttgat aagtggttca
     1261 aaaatccaat tctggtgagc cacatcgtct atatcaccgc cgacaagcag aagaacggca
     1321 tcaaggcgaa cttcaagatc caccacaacg tggaggacgg cagcgtgcag ctcgccgacc
     1381 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1441 tgagctatca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1501 tggagttcgt gaccgccacc acgaactctc tcggcatgga cgagctgtac aagggcggta
     1561 ccggagggag catgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1621 agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg
     1681 ccaccaacgg caagctaacc ctgaagctca tctgcaccac cggcaagctg cccgtgccct
     1741 ggcccaccct cgtgaccacc ctgggctacg gcgtgcagtg cttcgcccgc taccccgacc
     1801 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1861 ccatcagctt caaggacgac ggaacctaca agacccgcgc cgaggtgaag ttagagggcg
     1921 acaccctggt gaaccgcatc gagctaaagg gcatcgactt caaggaggac ggcaacatcc
     1981 tggggcacaa gctggagtac aactttaaca accccctgaa catgaatttc gaactgtcag
     2041 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaaataa gaattcgaag
     2101 cttgatccgg ctgctagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2161 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2221 tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2281 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     2341 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     2401 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     2461 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     2521 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2581 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2641 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     2701 cgctggcagt gtccctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     2761 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2821 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2881 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2941 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     3001 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     3061 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     3121 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacattatt
     3181 cagattgggc cccgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
