LOCUS       sequence-402524-       12616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BCL9 overexpression vector with hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12616)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 12616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: pLVX-Hyg-CMV-BCL9
FEATURES             Location/Qualifiers
     source          1..12616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(785..1295)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(848..866)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    1344..1461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1536..1560
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5875..5899)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5957..5981)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5978..6181)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6007..6027)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6182..6561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(6716..6964)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6774..6793)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6944..6964)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7262..7306)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7491..7724
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8217..8342
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8389..8569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8570..8796)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8824..8844)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8824..8842)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8863..8879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8863..8879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8876..8898)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8887..8903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8911..8917,8918..8935,8936..8941))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8956..8977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9094..9111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9265..9853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9345..9364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10024..10815,10816..10884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10647..10666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10885..10989)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11015..11470)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11152..11173)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11364..11383
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11597..11619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11611..11628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11612..11628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11638..11657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11638..11656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      11677..11812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    11852..11973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11863..11882)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11917..11936
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             12045..12278
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(12358..12616,1..767))
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGELSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
       61 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
      121 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
      181 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
      241 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
      301 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
      361 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc
      421 gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
      481 tgtgcacggc gggagatgca ataggtcagg ctctcgctca attccccaat gtcaagcact
      541 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
      601 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
      661 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg acacgctgtc gaacttttcg
      721 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt ggcccgggat
      781 ccccctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
      841 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
      901 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
      961 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     1021 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     1081 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     1141 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     1201 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     1261 cagggctgcc ttggaaaagg cgcaacccca accccctcga gccgcggcca aagtggatct
     1321 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     1381 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     1441 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctcga ggtcgagaat
     1501 tgggagagag ctatgacgtc gcatgcacgc gatcaaccac tttgtacaag aaagctgggt
     1561 tgcggccgca ctcgagctaa aacatcatgt ttcctgggtt gccaggtcct tggctaaatc
     1621 cacccatgcc cacgtcagca gccatgcccc ggggcctcat ctgtgggggg atcatgatgt
     1681 tctgttgggg tcccatcatg ccctgcatgg acatcatcat gcctggagag cccacaggcc
     1741 cagggtgggt gtagagcccg gcaggccccc gatccttgcc aggaatcatg cccacggcag
     1801 cagctggatt gctcatcagg gtgggctggc cgggcattgt agattgtgct ggtgacatca
     1861 tccgatggtg aggtcccatc atgccttgtt ggagaaaggc tggtggtctc atggggttgt
     1921 ggcctggcat ggatggagct gtaccaagag ggatgtccgg agttcccact ggccctggac
     1981 ctcccatgcc aggtaatgct agtcccattc tgggggcttg ttcgcccatc atcccctgca
     2041 tgtgtgaaaa cccaggtcca ggaccctggg gctgtttacg gcctggaact tcccctcgag
     2101 ggaaatattg cagcgtctgg ctgggcttct cagatggaat aatgcgggat agatcaaact
     2161 caggtattcc ggtggctcca ggtcggatga cctcctgcag atctgggtct gtaaacaccg
     2221 aaggcatgct gttccccagg acagtgaagg agtcaggacc cccggggcct ccaggcttgc
     2281 aaagtgctgc atctgctgaa ctttggggca ggttgctggg gcggccaagg gggccttctc
     2341 ctgggaaacc catccctcct gggaagctgc cctgcccccc actggggcca ttgtgaggga
     2401 atggaacctg ctgtggggga gactgtactg gagggaagcc ctgggggaag cccatccgtc
     2461 cttgaggtac catcggtggc tcctgggacc catgccccat gataggattg tgtgacatca
     2521 agccaggccc cattgggacc ccatgaggag gtatgttggg tcccacggca tttggagagg
     2581 gcatctgatt ggagtgagaa agtggctggg tcattcccat tgggctgagg gttggcatcg
     2641 gaaccacggg gtttggacct gaaattcgag gattctgtgt attaatgccc attcctggca
     2701 tattattcat tgatggcaag ttgggagaac gagctggagg ggagtcgtca tctgagctgg
     2761 ccacagtctt gatagcatca tggtataacg gggtggaact gggcattgca aacttggaca
     2821 ttcgagacat cataatagag agtgggttct gggaaagggt tggctctgga ggcatggtat
     2881 aaggtgtact agagggaaga cttccaggga tattcacaga ggcaggctgg ctggctgtag
     2941 gaggtggggg gccacctgac tctacatttc ccagcatggc tggggaggcc atggtgaggg
     3001 gtgctttatg gtttggaggg atcccaggac tctgaagggg aggttttgga gaagaggtcc
     3061 atccaggtga cggggcagga agtgatggag acttgaggtg gacaggtgaa gcagcagcag
     3121 accccaaaac agggggggac ttaatggaag cagcagcagc tgggcccgcc agcatgcctg
     3181 ccagctgcga tggagtctgg ggggacttga ggttccccga gggcgagccc agcattggtg
     3241 actggacttg gtgcatcgtg ggagacttca gagggttaat gcctggggaa tgcacctggc
     3301 tgcctgccac agatatatcc aagggcttcc gccccaggcc gcgctgaact ggaggagctg
     3361 tgttgaggct gatggggtta ctggaagggt tgagaggtag tggtggcata tgactgagcc
     3421 ggctgttagt cctctggtcg ggcccaattg gttctctgag attccgcaag ccactgttgc
     3481 tgcctggacc ctgagacatg ggaaggaatg gtctggggcc catgccatac tcctgctggg
     3541 ggtgctcacc aaatggcagt ggcaccatct tctgctgagc aggcagcatg tctgagccac
     3601 ctgggcgtaa tttcagcatc tcctcagggc ccgccccggc ctctctcatc ttctgaggta
     3661 tcatctgaga gttggatccc atgttgacat ttagattgac atctcccttc atcccactag
     3721 gaaccatccc aaactcaagt tcccgaccag ggcccatctg tgggggaatt ccttttggaa
     3781 agtcacccct agaaggactc agagggccct caactggtat tcgagggaaa atggggttat
     3841 tcccaggctc catgtggcgc tgggagcctg gaatcatcct gttcatctcc atgctgggcc
     3901 tgatgccttc catggccatc cctgggggga gacccagctg tttctctgcc agctgctgct
     3961 gaaacatctc ttcagacaat ccttgggggt ttgggaagcg ttcccctcgg ccaggaccgc
     4021 tgaaaatgcc ctggccagga ggaaaatttc gaccatctgg gatttttggc acatcatctg
     4081 gccaactgac tccagaaaga cctggtctag atgcagggtt ggggacattc ggcccttcca
     4141 tttcagagtt tatcatgcct gcaaatccag ggaggcgcat ctggctccct ggcatgttgg
     4201 ggtggggagc catgcccctc gggggcagag aatgtggcat gttgatacca tcagaaaatg
     4261 gctctgtacc cccaggtgcc cagccttcac taggggtcat ctggtatgga ggggggggtc
     4321 ctcggaccac tccccgaggc ccgtgctgat ggaccatcat gtcctggagg gaacactgct
     4381 ggacaaccac ttgttcctgc ttcctcctct tctcttcata aaactcctgc tgcagtttca
     4441 gccacgctat ctgctcggga gtcatatggt caaggtggtc gggtcctatg gtcccagact
     4501 gggagttcat agaaggtgga accatttcat ctggagaaaa gggtacatct ctatgtcctt
     4561 gagggccaaa tggagctccc acgtctgtcc ggggcccagg tcccttacct aggctttggg
     4621 attgggccat catggcctgt attggccctt ctggtttttt ctgaggccca tctaataccc
     4681 caggattctg ctgcggtccc ccactttgtg ctcctgtgaa ttctttctca tcaggaaaaa
     4741 gcatgcgctg gatatctctg agagtttgta aggagcgctc ccggtgctcc agctgctcct
     4801 gagataggcc atcgggattc tctcccagtg tggggggctc gccactggac actggtggag
     4861 gcggaggggc tttgggatct gctgaagagc tattgctccc ctgggagaca ggggtcactg
     4921 ccctattgtt gggagttgag ttgggcccag taccatctgg gggcagtgga gtggagctgg
     4981 caggacttcc tacagaagga atcagtttgt tttctacccc aggactctcc cggtccaggg
     5041 gacgtggggg tgcggcaggc ttgggtgctg gtgccggaat gggtggagtt ggctgcagtc
     5101 tggtattctg ggaagaattc tggtcctggt tggccggagc tgggggctgt tgtgggagag
     5161 gtttcggatc attccgaagg gcagatatct gtgtgttcag aggcgctgtg cttctctctg
     5221 tcttgttgtt agaaatgttc tggatgtgga aagagacgat agtttcaacc tggcccttca
     5281 aaacagcttc tgcagcttta ttggccatct cagtagaaaa cacgtacacc actttggctg
     5341 gagtcttctg agcaggtgtg ggctctgtgg cagtagtttg gccatgggag ggggtagaag
     5401 acctgggggc tgtagcattt gatggggtca tcgagtgtgg tgtgtgctgg gaatcctggg
     5461 actttatgtg gtcagcagaa ttacattctt taatgtcaga gtcatcgttt ggagtcccag
     5521 gatctctctg atcaaaggag tcggcggaaa tacttcgctc ccttttcccc ttgcccttgg
     5581 caccatttcc agccccattc ttcagcccca tgctcccacc tgggccaggg agtgctttag
     5641 gggtatggcc cccactcttg gagtcacagg gggatggctg ggattggctg gctgagcccc
     5701 cctgtttacc ctgattggag aatttggaat ccagctgggg gtttccagat ggggacatca
     5761 ctgtaggggg acggaccatc acctcctgct ttgacttagg gctactctgt gtgtttcctg
     5821 atggagagct cctcacttta gggttactgg aatgcatggt tcgaactcct tccaagcctg
     5881 cttttttgta caaacttgtt cttcgaatct agaatcttct atggaggtca aaacagcgtg
     5941 gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata tagacctccc
     6001 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     6061 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     6121 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     6181 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccgtaagg
     6241 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     6301 gacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     6361 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     6421 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     6481 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     6541 aactagtcaa taatcaatgt caacatggcg gtcatattgg acatgagcca atataaatgt
     6601 acatattatg atatagatac aacgtatgca atggccaata gccaatattg atttatgcta
     6661 tataaccaat gactaatatg gctaattgcc aatattgatt caatgtatag ataccggtgt
     6721 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     6781 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     6841 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
     6901 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     6961 cctcggtgaa gggggcggcc gctcgaggct agtctcgtga tcgataccgt cgagatccgt
     7021 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     7081 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     7141 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     7201 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     7261 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     7321 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     7381 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     7441 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     7501 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     7561 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     7621 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     7681 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     7741 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     7801 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     7861 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     7921 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     7981 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     8041 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     8101 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     8161 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     8221 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     8281 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     8341 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     8401 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     8461 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     8521 gttagccaga gagctcccag gctcagatct ggtctaacca gagagacccg tttatgtatc
     8581 gagctaggca cttaaataca atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc
     8641 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca
     8701 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca
     8761 tgttgcaaga ctacaagagt attgcataag actacattaa gcttgcagct ccagcttttg
     8821 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     8881 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8941 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9001 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9061 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10021 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10081 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10141 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10201 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10261 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10321 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10381 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10441 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10501 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10561 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10621 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10681 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10741 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10801 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10861 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10921 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10981 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
    11041 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
    11101 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
    11161 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
    11221 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
    11281 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
    11341 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
    11401 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    11461 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    11521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    11581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
    11641 tacgactcac tatagggcga attgggtacg tccctaggcc tccaaaaaag cctcctcact
    11701 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
    11761 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
    11821 ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt tggacaaacc
    11881 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
    11941 tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt
    12001 tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag agattttcca
    12061 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
    12121 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
    12181 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag
    12241 cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
    12301 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg tttaaaccta
    12361 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
    12421 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
    12481 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
    12541 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
    12601 ccgcggcgat cctgca
//
