LOCUS       sequence-402525-       10228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-ON 3rd generation expression vector with inducible expression of
            MCL1 and a puromycin selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: pLVX-TetOne-Puro-MCL1
FEATURES             Location/Qualifiers
     source          1..10228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    1631..1765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1809..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2187..2231)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2416..2649
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3146..3271
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3318..3951
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4119..4253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4301..4405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4406..4474,4475..5266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4624..4643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5437..6025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5926..5945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6179..6196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6313..6334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6349..6354,6355..6372,6373..6379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6387..6403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6392..6414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6411..6427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6411..6427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             6555..7188
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7395..7983
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7501..7512
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7910..7930
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7997..8596)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8080..8100)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8577..8596
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8605..8934)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8619..8754
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(8945..9691)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(9710..10220)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9773..9791)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaagaat tcggcatgtt tggcctcaaa agaaacgcgg taatcggact
      421 caacctctac tgtggggggg ccggcttggg ggccggcagc ggcggcgcca cccgcccggg
      481 agggcgactt ttggctacgg agaaggaggc ctcggcccgg cgagagatag ggggagggga
      541 ggccggcgcg gtgattggcg gaagcgccgg cgcaagcccc ccgtccaccc tcacgccaga
      601 ctcccggagg gtcgcgcggc cgccgcccat tggcgccgag gtccccgacg tcaccgcgac
      661 ccccgcgagg ctgcttttct tcgcgcccac ccgccgcgcg gcgccgcttg aggagatgga
      721 agccccggcc gctgacgcca tcatgtcgcc cgaagaggag ctggacgggt acgagccgga
      781 gcctctcggg aagcggccgg ctgtcctgcc gctgctggag ttggtcgggg aatctggtaa
      841 taacaccagt acggacgggt cactaccctc gacgccgccg ccagcagagg aggaggagga
      901 cgagttgtac cggcagtcgc tggagattat ctctcggtac cttcgggagc aggccaccgg
      961 cgccaaggac acaaagccaa tgggcaggtc tggggccacc agcaggaagg cgctggagac
     1021 cttacgacgg gttggggatg gcgtgcagcg caaccacgag acggccttcc aaggcatgct
     1081 tcggaaactg gacatcaaaa acgaagacga tgtgaaatcg ttgtctcgag tgatgatcca
     1141 tgttttcagc gacggcgtaa caaactgggg caggattgtg actctcattt cttttggtgc
     1201 ctttgtggct aaacacttga agaccataaa ccaagaaagc tgcatcgaac cattagcaga
     1261 aagtatcaca gacgttctcg taaggacaaa acgggactgg ctagttaaac aaagaggctg
     1321 ggatgggttt gtggagttct tccatgtaga ggacctagaa ggtggcatca ggaatgtgct
     1381 gctggctttt gcaggtgttg ctggagtagg agctggtttg gcatatctaa taagataaac
     1441 cggtgccggc ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
     1501 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
     1561 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
     1621 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
     1681 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
     1741 cattataagc tgcaataaac aagttaagtc gataaactgg atctctgctg tccctgtaat
     1801 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
     1861 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
     1921 taaaggcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
     1981 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     2041 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     2101 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     2161 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     2221 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     2281 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     2341 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     2401 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     2461 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     2521 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     2581 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     2641 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     2701 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     2761 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg
     2821 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     2881 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     2941 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
     3001 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
     3061 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
     3121 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
     3181 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
     3241 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
     3301 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     3361 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     3421 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     3481 gtctaaccag agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg
     3541 ctcgccactc cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc
     3601 ccttgtagca agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca
     3661 cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat
     3721 ccatcccatg caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt
     3781 ctaccttatc tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc
     3841 tgacccctgg ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga
     3901 tccacagatc aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag
     3961 tttgtagtac atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca
     4021 caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga
     4081 agtttgggat gattttggtt agcttgagtt atccagttga tccagacatg ataagataca
     4141 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttgatctcc
     4261 cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4381 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4441 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4501 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4561 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4621 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4681 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4741 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4801 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4861 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4921 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4981 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5041 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5101 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5161 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5221 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5281 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5341 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5581 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5821 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6061 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6121 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6181 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6241 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6301 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6361 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6421 ctatgaccat gattacgcca agctctagct agaggtcgac ggtaaacgct agcaatgtca
     6481 aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
     6541 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
     6601 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     6661 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     6721 taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc
     6781 gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct
     6841 tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt
     6901 gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca
     6961 tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta
     7021 ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga
     7081 cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc
     7141 acagatcaag gatatcttgt cttcgttggg agtgaattag cccttccagt cccctctttt
     7201 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtacct
     7261 gaggtgtgac tggaaaaccc acctcctcct cctcttgtgc ttctagccag gcacaatcag
     7321 cattggtagc tgctgtattg ctacttgtga ttgctccatg tttttctagg tctcgactgc
     7381 agaattaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     7441 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     7501 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     7561 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     7621 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     7681 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     7741 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     7801 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     7861 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     7921 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     7981 attgttccag acgcgttcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     8041 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     8101 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     8161 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     8221 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     8281 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     8341 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
     8401 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
     8461 cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
     8521 cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
     8581 gcttgtactc ggtcatggtc gcgttttgca aaagcctagg cctccaaaaa agcctcctca
     8641 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     8701 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     8761 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     8821 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     8881 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcgc
     8941 gcgtttaccc ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat
     9001 caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
     9061 cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     9121 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtt
     9181 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     9241 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     9301 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     9361 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     9421 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
     9481 ccagggggca ggagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     9541 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
     9601 cgagtttcct tgtcgtcagg ccttcgatac cgactccatt gagtaattcc agagcagagt
     9661 ttatgacttt gctcttgtcc agtctagaca tggtaattcg atgatccccc tggggagaga
     9721 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     9781 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     9841 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     9901 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     9961 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
    10021 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
    10081 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
    10141 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
    10201 aaaggcgcaa ccccaacccc gtggaatt
//
