LOCUS       sequence-402565-        4734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the photoproximity labeling catalyst LOV* fused to H2B in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Hananya N, Ye X, Koren S, Muir TW
  TITLE     A genetically encoded photoproximity labeling approach for mapping 
            protein territories.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2219339120. doi: 
            10.1073/pnas.2219339120. Epub 2023 Apr 10.
  PUBMED    37036999
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-18"; volume: "120(16):e2219339120. 
            doi"; pages: " 10.1073/pnas.2219339120. Epub 2023 Apr 10"
COMMENT     Sequence Label: pCMV-FLAG-LOV*-H2B
FEATURES             Location/Qualifiers
     source          1..4734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..249
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     206..226
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(251..355)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      382..837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(469..488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     679..700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    844..965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(855..874)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     909..928
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1807..1830)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1919..2122)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1948..1968)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2123..2426
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2601..3189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2681..2700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3518..3565
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3590..3609
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3797..4591)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAATDSQRIAFYRLLDEFF"
     primer_bind     complement(3908..3927)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4518..4537
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(4626..4734,1..249))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(4640..4734,1..41)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
       61 actatggttg ctgactaatt gagatgcata ctttgcatac ttctgcctgc tggggagcct
      121 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
      181 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt
      241 ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      361 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg
      841 ccttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      901 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      961 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1021 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
     1081 ggccgcttac ttagcgctgg tgtacttggt gacggccttg gtgccctcgg acacggcgtg
     1141 cttggccaac tccccgggca gcagcaggcg cacggccgtc tggatctccc tggaggtgat
     1201 ggtcgagcgc ttgttgtaat gcgccaggcg ggaagcctca cccgcgatgc gttcgaagat
     1261 gtcgttgacg aaggagttca tgattcccat ggccttagag gagatgccgg tgtcggggtg
     1321 gacctgcttc agcaccttgt acacgtatac ggagtagctc tccttgcggc tgcgcttgcg
     1381 cttcttgccg tccttcttct gcgccttagt cacggctttc ttcgagccct tcttgggcgc
     1441 gggagcggac ttcgctggtt ccggacctga gccacctcca tccaggagca ctccgatgaa
     1501 atactggagc tcacccttct ggtccctcat gggctggagg tgcaggaggt tccagaattt
     1561 cttcccgctt tttgtgtagt ttatcagctg cacggtaatc tcgcgctgat ctctaatggc
     1621 atcgcgaatc ttctgcactg tggcctggtc tgtctctggt ccctgcagaa agcggccatt
     1681 gcggcccagg atctcctctc tggaatactc ggtcagctcc aggaagccat cggatgcgaa
     1741 gatgatggga ttgtctggca gccgtggatc agttatcacg aaacttttct ccatgccaga
     1801 tccgcccttg tcgtcgtcgt ccttatagtc acccatggta ccgtcgactg cagaattcga
     1861 agcttgagct cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag
     1921 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     1981 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     2041 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     2101 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     2161 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     2221 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     2281 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     2341 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     2401 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     2461 ccgtaattga ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca
     2521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3001 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3241 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3301 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
     3361 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
     3421 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
     3481 acagagtgcc agccctgggg ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
     3541 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
     3601 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
     3661 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     3721 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     3781 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3841 aatcggtagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     3901 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     3961 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4021 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     4081 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     4141 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     4201 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     4261 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     4321 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     4381 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     4441 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4501 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4561 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     4621 cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4681 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atgg
//
