LOCUS       sequence-402566-        7050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the photoproximity labeling catalyst LOV* fused to MVP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Hananya N, Ye X, Koren S, Muir TW
  TITLE     A genetically encoded photoproximity labeling approach for mapping 
            protein territories.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2219339120. doi: 
            10.1073/pnas.2219339120. Epub 2023 Apr 10.
  PUBMED    37036999
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-18"; volume: "120(16):e2219339120. 
            doi"; pages: " 10.1073/pnas.2219339120. Epub 2023 Apr 10"
COMMENT     Sequence Label: pCMV-FLAG-LOV*-MVP
FEATURES             Location/Qualifiers
     source          1..7050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    33..80
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     105..124
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(312..1106)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAATDSQRIAFYRLLDEFF"
     primer_bind     complement(423..442)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1033..1052
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1141..1498)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1155..1290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1307..1498
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     1455..1475
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(1500..1604)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1631..2086
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1718..1737)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1928..1949
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2093..2214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2104..2123)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2158..2177
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5372..5395)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5484..5687)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5513..5533)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5688..5991
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6166..6754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6246..6265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgccagccc tggggccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
       61 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
      121 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
      181 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
      241 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
      301 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
      361 gtagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
      421 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
      481 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
      541 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
      601 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
      661 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
      721 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
      781 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
      841 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
      901 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
      961 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     1021 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     1081 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     1141 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     1201 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     1261 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     1321 tgactaattg agatgcatac tttgcatact tctgcctgct ggggagcctg gggactttcc
     1381 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     1441 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     1501 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1561 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1621 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1681 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1741 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1801 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1861 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1921 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1981 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2041 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttacgc cttaagatac
     2101 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2161 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2221 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     2281 aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgcttacc
     2341 gcagtacagg caccacgtgg ttgtctccag gagcttgagg ggcctgagca gactgggggg
     2401 atatcccctc cccagggctg ggcccactgg ccacccttct gcccaggggc tgaccctcgg
     2461 gccccatccc cagcagccca aaggctgtgt tgaagaggtt gatgggagtg gagccatcgg
     2521 tgatgagggt tgatttcagg cccagggact ggagcagttt tacctgcatc tcaggcccag
     2581 ccacagcaag gtccctgatg gtgctggggc ctatggcctc tgtcatctgc ttgaacttct
     2641 tcacctccac ctcagccagc tgctgagcct tgctcacctc cagctccagc tgggcccggg
     2701 catagaccag ttccagctct cggaccttct ggaccctctg gagctcagcc tccgtttcaa
     2761 tggccaaggc ctgtgctttt agcttggcct gcagcacgga cccttctccc tcaatccggg
     2821 ctgcctccgc acgggactcg gcctccgcct tggcagtccc ggtgctctcc acggccatgc
     2881 tcagagcctc cagctccaaa agttccttgc gagctttctc ggcttctgac tggtccagga
     2941 tcttctgccg ctcaagccgg ccgcgggctt cctgctccag tctctgagcc tcatgcttgg
     3001 ccgccgcttc ctgggagttg gtggtgatct cgatggccag ctggacgctg cgttgcaggg
     3061 cgtcccgggt cctctgatcc acaggctcca ctgactgcac gtccacactg ctgaccacca
     3121 gcccgttttg ggggaagaca gcctggtccc ggggcctggg cagggccatg ccatcggggc
     3181 ccttcgcttc cgaggtctca aagccaaaga cagcagtgcg aatgatgcgg gctgagttct
     3241 tatggaagtc atcgaaagtg acagaggcca cggccccccg cacccgggat gcgatggctt
     3301 tgcaggcatc acctacaaag tctggcactg aaaagagctt ggccgtctct tgggggtcct
     3361 tccggtcatt cacctcaaag tgccagttgt aggccagctg cagttgcagc ctggcatgat
     3421 ccgccgtttc gatggtgatg acgtctgtga agaagtcagg ccccagcagc aggcagagcg
     3481 cacggcgggc atggggacgc ttgggccgcc cagctgagag ggacaacact gtgaactgct
     3541 cctcaggacc cagcgacacc agctcaggcc cgaagaccac gcgggctcgc ttctctcggt
     3601 agtcgtacac ctgcaccgca gcgttgtggg gcacgcggta gctgaccaca cgggtcttgt
     3661 tccggggcgc caagggctgg aggctcttag ctgtgtcctt ctcacccctg tctgccagag
     3721 ggtcctgccc cttgttcagc agctcctcca ccccgggagg cagctctttc tcccacagga
     3781 cttcgtcctg ggtcagcatg taggtgcttc caatcacagc gcgcaccttt ccggtcttga
     3841 catcctgcac atagatgccc tcgttctcgt ctagagggat ggcctggcgc tcctccacca
     3901 cctccacttt ggcagatggc acatactcca ggggtccgcg gatgagccag tggtccccag
     3961 cctggtgtga gaccttctcc tcatcctccc cctcctccag gggctgcagg gccctcagca
     4021 gcagcccctg ctgctccgac agcacataca catcctggat gccttgttcc agctgctctc
     4081 ctggctggag gaaaaaagac ttctctccct tgaccacgcg cttctgcccc agctgattct
     4141 tgccatccgg tccgacaggg tcgagaatca cgcagtagtt gtgggggccc agggtggtga
     4201 tgggcacaac ccccagcacc tcctcgtgga catctggcac gtgggcctct gtgtcctgca
     4261 ctgttaccag ccactcctcc ccagtgcggc gggacactcc cctgaagtcc cggaagttcc
     4321 gccgagcccg gaggtgcagg gctgtctttt ccgtaaggat gacggcgtcc accaaatcca
     4381 gaacctcctc aaacaccgct gggaggtacg cccctactgt ggtgaccagc cattcttccc
     4441 ctgtcaccct ctccttgccg tcccggtccc agcactcctt gcgggccctg agccgcagag
     4501 cctggttctg cctgatgatg gtggcctgaa tgatctccac gacctccact tccttccggg
     4561 ggatgtacgt gccaggtccc tcgaaaagcc actcatctcc tgccaccacc ttgtctccat
     4621 ctttatcctc aaaatcaagc agcgccttta gatggagggc agtgttgggc agaaccacct
     4681 gcaggggtgt gatgtccttt tccagcacct cccctgggta cagggggaag gggtcctggg
     4741 ccagccggat ctcgaggtca gcgtggcgaa gccgaacttg ccctgtgaca tcaaacagca
     4801 ccaagccctg ggcatcccga gacacagggt tggccactgt gcagtagtga cgtgggggga
     4861 cggtcaccat gcgcatgggg gcaaacagta ccctctcatt gtcctgccgg atgtaggtct
     4921 ttggcccgac ctccacacgg gacacgttgc tgttctggtc cagcacatgg atatagtggt
     4981 atggggggat gcggatgatg aactcttcag ttgccattcc gctccctcca cctgagccac
     5041 ctccatccag gagcactccg atgaaatact ggagctcacc cttctggtcc ctcatgggct
     5101 ggaggtgcag gaggttccag aatttcttcc cgctttttgt gtagtttatc agctgcacgg
     5161 taatctcgcg ctgatctcta atggcatcgc gaatcttctg cactgtggcc tggtctgtct
     5221 ctggtccctg cagaaagcgg ccattgcggc ccaggatctc ctctctggaa tactcggtca
     5281 gctccaggaa gccatcggat gcgaagatga tgggattgtc tggcagccgt ggatcagtta
     5341 tcacgaaact tttctccatg ccagatccgc ccttgtcgtc gtcgtcctta tagtcaccca
     5401 tggtaccgtc gactgcagaa ttcgaagctt gagctcgaga tctgagtccg gtagcgctag
     5461 cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta
     5521 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     5581 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     5641 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     5701 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     5761 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     5821 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     5881 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     5941 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     6001 atatatgggc tatgaactaa tgaccccgta attgattact attaataact aatgcatggc
     6061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6901 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     6961 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     7021 ccaatggggt ctcggtgggg tatcgacaga
//
