LOCUS       sequence-402574-        9555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9555)
  TITLE     Unpublished huntingtin plasmids
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huntingtin_V2095-V3138_CbioCflag
FEATURES             Location/Qualifiers
     source          1..9555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      7554..7563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8237..8281
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8303..8326
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8348..8377
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             8378..8401
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8491..8511)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8909..8920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9145..9279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9182..9201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9236..9255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9308..9473
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggtga gtccggacaa
     5101 agactggtac gttcatcttg tcaaatccca gtgttggacc aggtcagatt ctgcactgct
     5161 ggaaggtgca gagctggtga atcggattcc tgctgaagat atgaatgcct tcatgatgaa
     5221 ctcggagttc aacctaagcc tgctagctcc atgcttaagc ctagggatga gtgaaatttc
     5281 tggtggccag aagagtgccc tttttgaagc agcccgtgag gtgactctgg cccgtgtgag
     5341 cggcaccgtg cagcagctcc ctgctgtcca tcatgtcttc cagcccgagc tgcctgcaga
     5401 accggcggcc tactggagca agttgaatga tctgtttggg gatgctgcac tgtatcagtc
     5461 cctgcccact ctggcccggg ccctggcaca gtacctggtg gtggtctcca aactgcccag
     5521 tcatttgcac cttcctcctg agaaagagaa ggacattgtg aaattcgtgg tggcaaccct
     5581 tgaggccctg tcctggcatt tgatccatga gcagatcccg ctgagtctgg atctccaggc
     5641 agggctggac tgctgctgcc tggccctgca gctgcctggc ctctggagcg tggtctcctc
     5701 cacagagttt gtgacccacg cctgctccct catccactgt gtgcacttca tcctggaggc
     5761 cgttgcagtg cagcctggag agcagcttct tagtccagaa agaaggacaa ataccccaaa
     5821 agccatcagc gaggaggagg aggaagtaga tccaaacaca cagaatccta agtatatcac
     5881 tgcagcctgt gagatggtgg cagaaatggt ggagtctctg cagtcggtgt tggccttggg
     5941 tcataaaagg aatagcggcg tgccggcgtt tctcacgcca ttgctcagga acatcatcat
     6001 cagcctggcc cgcctgcccc ttgtcaacag ctacacacgt gtgcccccac tggtgtggaa
     6061 gcttggatgg tcacccaaac cgggagggga ttttggcaca gcattccctg agatccccgt
     6121 ggagttcctc caggaaaagg aagtctttaa ggagttcatc taccgcatca acacactagg
     6181 ctggaccagt cgtactcagt ttgaagaaac ttgggccacc ctccttggtg tcctggtgac
     6241 gcagcccctc gtgatggagc aggaggagag cccaccagaa gaagacacag agaggaccca
     6301 gatcaacgtc ctggccgtgc aggccatcac ctcactggtg ctcagtgcaa tgactgtgcc
     6361 tgtggccggc aacccagctg taagctgctt ggagcagcag ccccggaaca agcctctgaa
     6421 agctctcgac accaggtttg ggaggaagct gagcattatc agagggattg tggagcaaga
     6481 gattcaagca atggtttcaa agagagagaa tattgccacc catcatttat atcaggcatg
     6541 ggatcctgtc ccttctctgt ctccggctac tacaggtgcc ctcatcagcc acgagaagct
     6601 gctgctgcag atcaaccccg agcgggagct ggggagcatg agctacaaac tcggccaggt
     6661 gtccatacac tccgtgtggc tagggaacag catcacaccc ctgagggagg aggaatggga
     6721 cgaggaagag gaggaggagg ccgacgcccc tgcaccttcg tcaccaccca cgtctccagt
     6781 caactccagg aaacaccggg ctggagttga catccactcc tgttcgcagt ttttgcttga
     6841 gttgtacagc cgctggatcc tgccgtccag ctcagccagg aggaccccgg ccatcctgat
     6901 cagtgaggtg gtcagatccc ttctagtggt ctcagacttg ttcaccgagc gcaaccagtt
     6961 tgagctgatg tatgtgacgc tgacagaact gcgaagggtg cacccttcag aagacgagat
     7021 cctcgctcag tacctggtgc ctgccacctg caaggcagct gccgtccttg ggatggacaa
     7081 ggccgtggcg gagcctgtca gccgcctgct ggagagcacg ctcaggagca gccacctgcc
     7141 cagcagggtt ggagccctgc acggcatcct ctatgtgctg gagtgcgacc tgctggacga
     7201 cactgccaag cagctcatcc cggtcatcag cgactatctc ctctccaacc tgaaagggat
     7261 cgcccactgc gtgaacattc acagccagca gcacgtactg gtcatgtgtg ccactgcgtt
     7321 ttacctcatt gagaactatc ctctggacgt agggccggaa ttttcagcat caataataca
     7381 gatgtgtggg gtgatgctgt ctggaagtga ggagtccacc ccctccatca tttaccactg
     7441 tgccctcaga ggcctggagc gcctcctgct ctctgagcag ctctcccgcc tggatgcaga
     7501 atcgctggtc aagctgagtg tggacagagt gaacgtgcac agcccgcacc gggccatggc
     7561 ggctctgggc ctgatgctca cctgcatgta cacaggaaag gagaaagtca gtccgggtag
     7621 aacttcagac cctaatcctg cagcccccga cagcgagtca gtgattgttg ctatggagcg
     7681 ggtatctgtt ctttttgata ggatcaggaa aggctttcct tgtgaagcca gagtggtggc
     7741 caggatcctg ccccagtttc tagacgactt cttcccaccc caggacatca tgaacaaagt
     7801 catcggagag tttctgtcca accagcagcc atacccccag ttcatggcca ccgtggtgta
     7861 taaggtgttt cagactctgc acagcaccgg gcagtcgtcc atggtccggg actgggtcat
     7921 gctgtccctc tccaacttca cgcagagggc cccggtcgcc atggccacgt ggagcctctc
     7981 ctgcttcttt gtcagcgcgt ccaccagccc gtgggtcgcg gcgatcctcc cacatgtcat
     8041 cagcaggatg ggcaagctgg agcaggtgga cgtgaacctt ttctgcctgg tcgccacaga
     8101 cttctacaga caccagatag aggaggagct cgaccgcagg gccttccagt ctgtgcttga
     8161 ggtggttgca gccccaggaa gcccatatca ccggctgctg acttgtttac gaaatgtcgg
     8221 tggaagcggt ggatcaggat taaacgatat attcgaagca caaaagattg agtggcacga
     8281 aggttctgct ggcgggtccg gggattacaa ggatgacgac gataagtgaa acctctactt
     8341 ccaatcgcac catcatcacc atcaccatca ccaccatgat tacaaggatg acgacgataa
     8401 gtgaggcggc cgccaacttg atatcaagct tatcgataat caacctctgg attacaaaat
     8461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8821 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8941 cgggcgcccc cctcctcacg gcgagcgaag cttgtcgaga agtactagag gatctctaga
     9001 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     9061 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9241 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
     9301 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     9361 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     9421 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     9481 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     9541 atcttcggct acttt
//
