LOCUS       sequence-402604-       10209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for human AAVS1 locus knock-in, constitutive 
            expression of GEM2-Halo-FKBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, 
            Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J
  TITLE     Genetically encoded multimeric tags for subcellular protein 
            localization in cryo-EM.
  JOURNAL   Nat Methods. 2023 Dec;20(12):1900-1908. doi: 
            10.1038/s41592-023-02053-0. Epub 2023 Nov 6.
  PUBMED    37932397
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02053-0"; journalName: "Nat Methods"; date: 
            "2023-12"; volume: "20"; issue: "12"; pages: "1900-1908"
COMMENT     Sequence Label: pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..10209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(259..363)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      390..845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(477..496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     687..708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    1362..2165
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    2191..2239
                     /note="synthetic polyadenylation signal"
     misc_feature    2253..2344
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2293..2312
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2356..3518
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2587..3509
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3466..3486
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     intron          3557..3689
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3672..3691
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3734..3752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3770..3779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4745..5632
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             5675..5998
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    6012..6578
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6028..6580
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6172..6189)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6399..6418
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6580..6981
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MGAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
                     IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7139..7260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7176..7195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7230..7249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    7296..8132
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8677..8694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8848..9436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8928..8947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9607..10209,1..189,190..258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
       61 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      361 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
      421 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      481 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      541 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      601 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      661 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      721 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      781 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
      841 aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      901 ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt
      961 acttggagcg gccgtcgacc tactctcttc cgcattggag tcgctttaac tggccctggc
     1021 tttggcagcc tgtgctgacc catgcagtcc tccttaccat ccctccctcg acttcccctc
     1081 ttccgatgtt gagcccctcc agccggtcct ggactttgtc tccttccctg ccctgccctc
     1141 tcctgaacct gagccagctc ccatagctca gtctggtcta tctgcctggc cctggccatt
     1201 gtcactttgc gctgccctcc tctcgccccc gagtgccctt gctgtgccgc cggaactctg
     1261 ccctctaacg ctgccgtctc tctcctgagt ccggaccact ttgagctcta ctggcttctg
     1321 cgccgcctct ggcccactgt ttccccttcc caggcaggtc ctgctttctc tgacctgcat
     1381 tctctcccct gggcctgtgc cgctttctgt ctgcagcttg tggcctgggt cacctctacg
     1441 gctggcccag atccttccct gccgcctcct tcaggttccg tcttcctcca ctccctcttc
     1501 cccttgctct ctgctgtgtt gctgcccaag gatgctcttt ccggagcact tccttctcgg
     1561 cgctgcacca cgtgatgtcc tctgagcgga tcctccccgt gtctgggtcc tctccgggca
     1621 tctctcctcc ctcacccaac cccatgccgt cttcactcgc tgggttccct tttccttctc
     1681 cttctggggc ctgtgccatc tctcgtttct taggatggcc ttctccgacg gatgtctccc
     1741 ttgcgtcccg cctccccttc ttgtaggcct gcatcatcac cgtttttctg gacaacccca
     1801 aagtaccccg tctccctggc tttagccacc tctccatcct cttgctttct ttgcctggac
     1861 accccgttct cctgtggatt cgggtcacct ctcactcctt tcatttgggc agctccccta
     1921 ccccccttac ctctctagtc tgtgctagct cttccagccc cctgtcatgg catcttccag
     1981 gggtccgaga gctcagctag tcttcttcct ccaacccggg cccctatgtc cacttcagga
     2041 cagcatgttt gctgcctcca gggatcctgt gtccccgagc tgggaccacc ttatattccc
     2101 agggccggtt aatgtggctc tggttctggg tacttttatc tgtcccctcc accccacagt
     2161 ggggcgcatg cgtggaatta tcacctcgag aataaaatat ctttattttc attacatctg
     2221 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     2281 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     2341 ctctactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2401 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     2461 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     2521 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     2581 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     2641 tgcgtgcctt gaattacttc cacctggctc cagtacgtga ttcttgatcc cgagctggag
     2701 ccaggggcgg gccttgcgct ttaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     2761 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     2821 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     2881 aagatagtct tgtaaatgcg ggccaggatc tgcacactgg tatttcggtt tttggggccg
     2941 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3001 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3061 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3121 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctcc agggggctca aaatggagga
     3181 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaggg gcctttccgt
     3241 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3301 agttctggag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3361 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3421 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     3481 tggttcaaag tttttttctt ccatttcagg tgtcgtgaac acgggctgca gaagttggtc
     3541 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     3601 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     3661 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     3721 aggctagagt acttaatacg actcactata ggctagcctc gagaattttg ccaccatggg
     3781 atccaccgat aacgcccctc agctggccct gcgggatgtg gcagctagac agctggctaa
     3841 tgccaccaaa accgtgcccc aactgcgcac catcacaccc agatggctgg tcagactgct
     3901 gcactggacc cctgtggaag ccggcatcta ccgggttaac caggtgaagg acgcctccca
     3961 gattaccgtc gcttgtagcg agagagatga gagcgagctg cccgaaacct tcgtggacta
     4021 catcgacaat cctagagagt acctgctgag cgccgtgaac acagtggtgg acgtgcatac
     4081 cagaatcagc gacctgtata gcaaccccca cgaccagatc cgggaacagc tcagactgac
     4141 aatcgagatc atgaaagaaa gacaagagtc cgagctgatc aacagcagag aatacggcct
     4201 gctgaacaac gtggcccccg gccagctggt gcacacaaga aacggcgccc caacacctga
     4261 cgacctggac gagctgctga tcagagtgtg gaaggaacct gctttttttc tggcccaccc
     4321 tcaggccatc gccgccttcg gcagagagtg cacacggcgg ggcgtgccac ctgccacagt
     4381 gtccctgttc ggcagcagct tcatcacctg gcggggcgtg cctctgatcc ccagcgacaa
     4441 ggtgcctctg gaaaacggta agaccaagat cctgctgctc agggtcggag agtctagaca
     4501 gggagttgtg ggactgtacc agcctaatct gcctggcgag cagggcatgg gcctgtctgt
     4561 gcggttcatg ggcatcaacc ggaaggccct ggccagctac ctggtgtctc tgtactgcag
     4621 cctggccgtg ctgaccgatg atgccctggc cgtgctggat aacgtggacg tgacccagta
     4681 ccacacctac agatacaacg gtaccggcgg cggaggcagc ggcggcggat ctggcggcgg
     4741 cagcgcagaa atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga
     4801 gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg
     4861 taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg
     4921 ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt
     4981 cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt
     5041 cgtcctggtc attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc
     5101 agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga
     5161 atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa
     5221 gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc
     5281 gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga
     5341 gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc
     5401 gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg
     5461 gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc
     5521 taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga
     5581 cctgatcggc agcgagatcg cgcgctggct gtctactctg gagatttccg gtaagcttgg
     5641 aagcgccggc tctgccgctg gcagcggcga gggcggagtg caggtggaaa ccatctcccc
     5701 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat
     5761 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat
     5821 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
     5881 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
     5941 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaataggc
     6001 tctagcccaa ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     6061 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     6121 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     6181 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     6241 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     6301 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6361 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6421 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6481 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     6541 acggggacgt ggttttcctt tgaaaaacac gatgataata tgggcgccaa gcctttgtct
     6601 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     6661 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     6721 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     6781 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     6841 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     6901 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     6961 ggttatgtgt gggagggcta agcacttcgt ggccgaggag caggactgac acgtgctacg
     7021 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7081 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7141 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7201 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7261 tcatgtctgt ataccgtcga cctctagtat gcatcactag ggacaggatt ggtgacagaa
     7321 aagccccatc cttaggcctc ctccttccta gtctcctgat attgggtcta acccccacct
     7381 cctgttaggc agattcctta tctggtgaca cacccccatt tcctggagcc atctctctcc
     7441 ttgccagaac ctctaaggtt tgcttacgat ggagccagag aggatcctgg gagggagagc
     7501 ttggcagggg gtgggaggga agggggggat gcgtgacctg cccggttctc agtggccacc
     7561 ctgcgctacc ctctcccaga acctgagctg ctctgacgcg gctgtctggt gcgtttcact
     7621 gatcctggtg ctgcagcttc cttacacttc ccaagaggag aagcagtttg gaaaaacaaa
     7681 atcagaataa gttggtcctg agttctaact ttggctcttc acctttctag tccccaattt
     7741 atattgttcc tccgtgcgtc agttttacct gtgagataag gccagtagcc agccccgtcc
     7801 tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa actccctttg tgagaatggt
     7861 gcgtcctagg tgttcaccag gtcgtggccg cctctactcc ctttctcttt ctccatcctt
     7921 ctttccttaa agagtcccca gtgctatctg ggacatattc ctccgcccag agcagggtcc
     7981 cgcttcccta aggccctgct ctgggcttct gggtttgagt ccttggcaag cccaggagag
     8041 gcgctcaggc ttccctgtcc cccttcctcg tccaccatct catgcccctg gctctcctgc
     8101 cccttcccta caggggttcc tggctctgct cttcagactg agccccgttc ccctgcatcc
     8161 ccgttcccct gcatccccct tcccctgcat cccccagagg ccccaggcca cctacttggc
     8221 ctggacccca cgagaggcca ccccagccct gtctaccagg ctgccttttg ggtggattct
     8281 cctccaactg tggggtgact gcttggcaaa ctcactcttc ggggtatccc aggaggcctg
     8341 gagcattggg gtgggctggg gttcagagag gagggattcc cttctcaggt tacgtggcca
     8401 agaagcaggg gagctgggtt tgggtcaggt ctgggtgtgg ggtgaccagc ttatgctgtt
     8461 tgcccaggac agcctagttt tagcgctgaa accctcagtc ctaggaaaac agggatggtt
     8521 ggtcactgtc tctgggctcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     8581 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     8641 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10201 gagttgctc
//
