LOCUS       sequence-402609-        5652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Hananya N, Ye X, Koren S, Muir TW
  TITLE     A genetically encoded photoproximity labeling approach for mapping 
            protein territories.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2219339120. doi: 
            10.1073/pnas.2219339120. Epub 2023 Apr 10.
  PUBMED    37036999
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-18"; volume: "120(16):e2219339120. 
            doi"; pages: " 10.1073/pnas.2219339120. Epub 2023 Apr 10"
COMMENT     Sequence Label: pCMV-PDK1-FLAG-LOV*
FEATURES             Location/Qualifiers
     source          1..5652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(199..993)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAATDSQRIAFYRLLDEFF"
     primer_bind     complement(310..329)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     920..939
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1028..1385)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1042..1177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1194..1385
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     1342..1362
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(1387..1491)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1518..1973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1605..1624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1815..1836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1980..2101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1991..2010)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2045..2064
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2556..2579)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3973..4176)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4002..4022)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4177..4480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4655..5243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4735..4754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5572..5619
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     join(5644..5652,1..11)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc
       61 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
      121 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
      181 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
      241 cgaatcggta gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
      301 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
      361 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
      421 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
      481 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
      541 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
      601 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
      661 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
      721 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
      781 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
      841 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
      901 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
      961 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     1021 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1081 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1141 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1201 tggttgctga ctaattgaga tgcatacttt gcatacttct gcctgctggg gagcctgggg
     1261 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     1321 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     1381 ctcaggactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1501 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttacgcctt
     1981 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2041 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2101 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2161 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc
     2221 gcttatccat ccaggagcac tccgatgaaa tactggagct cacccttctg gtccctcatg
     2281 ggctggaggt gcaggaggtt ccagaatttc ttcccgcttt ttgtgtagtt tatcagctgc
     2341 acggtaatct cgcgctgatc tctaatggca tcgcgaatct tctgcactgt ggcctggtct
     2401 gtctctggtc cctgcagaaa gcggccattg cggcccagga tctcctctct ggaatactcg
     2461 gtcagctcca ggaagccatc ggatgcgaag atgatgggat tgtctggcag ccgtggatca
     2521 gttatcacga aacttttctc catgccagat ccgcccttgt cgtcgtcgtc cttatagtca
     2581 ccggcactgc ggaacgtcgt catgtctttg ggttctctgc tggggacgca ccagtcatca
     2641 gcctcgtggt tggtgttgta atgcttccag gcagctttgt tatacactgg gagtctttct
     2701 attgagtctg ttgacagagc cttaatgtag ataactgcat ctgtcccgta accctctagg
     2761 gaatacagct tcaggtctcc ttggaagtat tgtgcgtaaa gacgtgatat gggcaatcca
     2821 taaccaaaac cagccagagg cactgcgcgg gaggtctcaa cacgaggtct tggtgcagtt
     2881 gaatacatgt agttgaaaag tctgtcaatt ttcctcaaag gaacgccacc tcctcggtca
     2941 ctcatcttca cagtcaaatc ctcattaccc agcgtgacat gaacttgaat aggggggtaa
     3001 acacctctgt tggcatggtg ttccatagtg gctctcattg cattcttgaa aagttcaaac
     3061 accatgtgat agagatggga tggtacataa accacttgta ttggctgtcc tggtgatttt
     3121 gcatttagtt cttcaagttc tagttcggga gagttaatat aatacaaatc acacagacgc
     3181 ctagcatttt catagccatc tttaataact tcaagtacat tgcagtttgg atttatgctt
     3241 ccaatgtgtt ttcgatgaga tggacttcct ttgccttttc caccaaacaa taaagagtgc
     3301 tgattgagta acattctaat tgaaatgcga ctcatgtaga atcgatccaa aaagtactga
     3361 acattctggc tggtgacagg atccacccca aagctctcct tgtattcaat cacaccctgg
     3421 gccattgtgg gaatgacatc attgtgtcgg tttctgatcc gtatcacagt atctgtaaag
     3481 tcataaatag ctttagcatc ctcagcactt ttgtccttaa aatcaagaag ctcctgaaga
     3541 ctctggatat accagctttg taccaattga acggatggtg tcctgagaag attatctgga
     3601 aggagactta tttctttcat tatatttgcc agtctgacag gcaactcttg ccgcagaaac
     3661 ataaatgagg tcttttcaca agcattcact gatccgaagt ccaggaactg cttcatggag
     3721 agcggggacg gcgagaagcg cgcgtagaag tccacctggc ccggaacgcc gcgctcggac
     3781 gccgggctgg agcccgagtc cgagctgaag ctgcggctga agccggcggc gcgcagcccc
     3841 gggcccgggc cggccaaggc ggctccgcga agcagccgcg ccagcctcat ggtaccgtcg
     3901 actgcagaat tcgaagcttg agctcgagat ctgagtccgg tagcgctagc ggatctgacg
     3961 gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     4021 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     4081 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     4141 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     4201 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     4261 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     4321 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     4381 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     4441 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     4501 atgaactaat gaccccgtaa ttgattacta ttaataacta atgcatggcg gtaatacggt
     4561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac
     5401 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     5461 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     5521 tcggtggggt atcgacagag tgccagccct ggggccgaac cccgcgttta tgaacaaacg
     5581 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     5641 gtattgtctc ct
//
