LOCUS       sequence-402613-        4419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the photoproximity labeling catalyst LOV* fused to the 
            mitochondria targeting sequence (MTS) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Hananya N, Ye X, Koren S, Muir TW
  TITLE     A genetically encoded photoproximity labeling approach for mapping 
            protein territories.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2219339120. doi: 
            10.1073/pnas.2219339120. Epub 2023 Apr 10.
  PUBMED    37036999
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-18"; volume: "120(16):e2219339120. 
            doi"; pages: " 10.1073/pnas.2219339120. Epub 2023 Apr 10"
COMMENT     Sequence Label: pCMV-MTS-FLAG-LOV*
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(199..993)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAATDSQRIAFYRLLDEFF"
     primer_bind     complement(310..329)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     920..939
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1028..1385)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1042..1177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1194..1385
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     1342..1362
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(1387..1491)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1518..1973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1605..1624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1815..1836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1980..2101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1991..2010)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2045..2064
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2556..2579)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2740..2943)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2769..2789)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2944..3247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3422..4010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3502..3521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4339..4386
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     join(4411..4419,1..11)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc
       61 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
      121 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
      181 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
      241 cgaatcggta gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
      301 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
      361 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
      421 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
      481 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
      541 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
      601 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
      661 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
      721 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
      781 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
      841 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
      901 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
      961 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     1021 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1081 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1141 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1201 tggttgctga ctaattgaga tgcatacttt gcatacttct gcctgctggg gagcctgggg
     1261 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     1321 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     1381 ctcaggactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1501 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttacgcctt
     1981 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2041 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2101 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2161 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc
     2221 gcttatccat ccaggagcac tccgatgaaa tactggagct cacccttctg gtccctcatg
     2281 ggctggaggt gcaggaggtt ccagaatttc ttcccgcttt ttgtgtagtt tatcagctgc
     2341 acggtaatct cgcgctgatc tctaatggca tcgcgaatct tctgcactgt ggcctggtct
     2401 gtctctggtc cctgcagaaa gcggccattg cggcccagga tctcctctct ggaatactcg
     2461 gtcagctcca ggaagccatc ggatgcgaag atgatgggat tgtctggcag ccgtggatca
     2521 gttatcacga aacttttctc catgccagat ccgcccttgt cgtcgtcgtc cttatagtca
     2581 cccttgtggg cgcgcacgca cacgctggtg ctgatggcgc gcttgcccac caggctgaac
     2641 acgcgggtgg ccagcatggt accgtcgact gcagaattcg aagcttgagc tcgagatctg
     2701 agtccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
     2761 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     2821 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     2881 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     2941 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     3001 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     3061 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     3121 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     3181 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     3241 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     3301 ataactaatg catggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3361 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3421 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3541 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3601 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3661 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3721 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3781 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3841 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3901 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3961 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4021 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4081 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4141 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt
     4201 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa
     4261 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
     4321 gccgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta
     4381 ttgccgtcat agcgcgggtt ccttccggta ttgtctcct
//
