LOCUS       sequence-402625-       11045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for 
            monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Tanida I, Ueno T, Uchiyama Y
  TITLE     A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged
            Human LC3 for the Monitoring of Mammalian Autophagy.
  JOURNAL   PLoS One. 2014 Oct 23;9(10):e110600. doi: 
            10.1371/journal.pone.0110600. eCollection 2014.
  PUBMED    25340751
REFERENCE   2  (bases 1 to 11045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-23"; volume: "9(10):e110600. doi"; pages: " 
            10.1371/journal.pone.0110600. eCollection 2014"
COMMENT     Sequence Label: FUGW-PK-hLC3
FEATURES             Location/Qualifiers
     source          1..11045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      179..314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     241..260
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        376..423
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             442..816
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    946..1067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(983..1002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1037..1056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1116..1132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1116..1132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1129..1151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1140..1156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1164..1170,1171..1188,1189..1194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1209..1230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1347..1364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1518..2106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1598..1617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2277..3068,3069..3137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2900..2919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3138..3242)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3317..3336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3508..3887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3889..4087
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4042..4062
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4105..4285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4332..4457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4950..5183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5368..5412
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5710..5827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5892..7103
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6940..6959
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7150..7863
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7180..7208)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7803..7823
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             7882..8577
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             8605..8979
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     misc_feature    9007..9595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9060..9080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9478..9489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9598..9614)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9599..9615)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10120..10300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10326..10343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10332..10556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10602..11030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10689..10708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10899..10920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(11039..11045,1..14))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11044..11045,1..328)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
       61 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      241 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
      301 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
      361 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
      421 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
      481 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
      541 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
      601 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
      661 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
      721 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
      781 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
      841 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
      901 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
      961 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1021 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     1081 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1141 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1201 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1261 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1321 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1381 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1441 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1501 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1561 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1621 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1681 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1741 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1801 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1861 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1921 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1981 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2101 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2161 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2221 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2281 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2341 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2401 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2461 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2521 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2581 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2641 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2701 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2761 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2821 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2881 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2941 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3001 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3061 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3121 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3181 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3241 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     3301 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     3361 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     3421 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     3481 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     3541 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3601 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3661 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3721 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3781 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3841 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3901 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3961 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4021 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4081 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     4141 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4201 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4261 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4321 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4381 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4441 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4501 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4561 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4621 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4681 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4741 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4801 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4861 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4921 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4981 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5041 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5101 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5161 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5221 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5281 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5341 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5401 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5461 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5521 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5581 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5641 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     5701 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     5761 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     5821 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta acccgtgtcg
     5881 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     5941 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     6001 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     6061 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     6121 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     6181 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     6241 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     6301 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     6361 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     6421 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     6481 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     6541 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     6601 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     6661 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     6721 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     6781 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     6841 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     6901 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     6961 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     7021 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     7081 cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag aggatccgct
     7141 agcgccacca tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     7201 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     7261 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     7321 ccaacacttg tcactacgtt aacttatggt gttcaatgct tttcaagata cccagatcat
     7381 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttacgtaca ggaaagaact
     7441 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     7501 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     7561 ggacacaaat tggaatacaa ctataacgat caccaggtgt acatcatggc agacaaacaa
     7621 aagaatggaa tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg aggcgttcaa
     7681 ctagcagacc attatcaaca aaatactcca attggcgatg ggcccgtcct tttaccagac
     7741 aaccattacc tgtttacaac ttctactctt tcgaaagatc ccaacgaaaa gagagaccac
     7801 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     7861 aaaggtggag gaggtagatc catggtgagc gagctgatta aggagaacat gcacatgaag
     7921 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
     7981 aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc
     8041 ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac
     8101 acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga
     8161 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     8221 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
     8281 atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc
     8341 ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc
     8401 aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
     8461 tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     8521 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagaggt
     8581 ggaggaggtt ccggactcag atctatgccg tcggagaaga ccttcaagca gcgccgcacc
     8641 ttcgaacaaa gagtagaaga tgtccgactt attcgagagc agcatccaac caaaatcccg
     8701 gtgataatag aacgatacaa gggtgagaag cagcttcctg ttctggataa aacaaagttc
     8761 cttgtacctg accatgtcaa catgagtgag ctcatcaaga taattagaag gcgcttacag
     8821 ctcaatgcta atcaggcctt cttcctgttg gtgaacggac acagcatggt cagcgtctcc
     8881 acaccaatct cagaggtgta tgagagtgag aaagatgaag atggattcct gtacatggtc
     8941 tacgcctccc aggagacgtt cgggatgaaa ttgtcagtgt aagaattcga tatcaagctt
     9001 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     9601 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     9661 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     9721 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9781 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     9841 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     9901 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     9961 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
    10021 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
    10081 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
    10141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10261 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
    10321 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
    10381 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
    10441 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
    10501 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
    10561 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
    10621 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10681 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10741 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10801 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10861 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10921 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    10981 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
    11041 aatgt
//
