LOCUS       sequence-402628-       11152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of guide RNAs, the SAM transcriptional activator for 
            CRISPRa and Cre in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Gaglia G, Burger ML, Ritch CC, Rammos D, Dai Y, Crossland GE, Tavana
            SZ, Warchol S, Jaeger AM, Naranjo S, Coy S, Nirmal AJ, Krueger R, 
            Lin JR, Pfister H, Sorger PK, Jacks T, Santagata S
  TITLE     Lymphocyte networks are dynamic cellular communities in the 
            immunoregulatory landscape of lung adenocarcinoma.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):871-886.e10. doi: 
            10.1016/j.ccell.2023.03.015. Epub 2023 Apr 13.
  PUBMED    37059105
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.03.015"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "871-886.e10"
COMMENT     Sequence Label: Lenti-SAM-Cre
FEATURES             Location/Qualifiers
     source          1..11152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(2675..2693)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(2745..2763)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(2801..3049)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2859..2878)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3029..3049)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3088..3587
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3123..3146)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3520..3539
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3612..3621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3621..4007
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4062..4082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4098..4640
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4665..5036
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             5052..5105
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5109..5129
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5130..6158
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    6244..6832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6297..6317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6715..6726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6904..7137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7163..7180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7169..7393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7439..7867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7526..7545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7736..7757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7876..7896)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7881..8210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8061..8196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8123..8142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8258..8305
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8324..8698
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8828..8949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8865..8884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8919..8938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8998..9014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8998..9014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9011..9033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9022..9038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9046..9052,9053..9070,9071..9076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9091..9112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9229..9246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9400..9988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9480..9499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10159..10950,10951..11019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10782..10801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11020..11124)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttaaa
     2641 aaaagcaccg actcggtgcc acttggccct gcagacatgg gtgatcctca tgttggccaa
     2701 gttgataacg gactagcctt attttaactt gctaggccct gcagacatgg gtgatcctca
     2761 tgttggccta gctctaaaac agagacggta tcccgtctcc ggtgtttcgt cctttccaca
     2821 agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca
     2881 ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg
     2941 tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc tctctaacag
     3001 ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctca aaccgctgtt
     3061 cctaggaatc ccgaggccta ttctaccggg taggggaggc gcttttccca aggcagtctg
     3121 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     3181 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     3241 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     3301 gcaggacgtg acaaatggaa gtagcacgtg tcactagtct cgtgcagatg gacagcaccg
     3361 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3421 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg nnngctcagg ggcgggctca
     3481 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3541 gcacgtctgc cgcgctgttc tcctcttcct catctccggg agacccgaca ttagcgctac
     3601 agcttaagcg ggccaccatg gcttcaaact ttactcagtt cgtgctcgtg gacaatggtg
     3661 ggacagggga tgtgacagtg gctccttcta atttcgctaa tggggtggca gagtggatca
     3721 gctccaactc acggagccag gcctacaagg tgacatgcag cgtcaggcag tctagtgccc
     3781 agaagagaaa gtataccatc aaggtggagg tccccaaagt ggctacccag acagtgggcg
     3841 gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa catggagctc actatcccaa
     3901 ttttcgctac caattctgac tgtgaactca tcgtgaaggc aatgcagggg ctcctcaaag
     3961 acggtaatcc tatcccttcc gccatcgccg ctaactcagg tatctacagc gctggaggag
     4021 gtggaagcgg aggaggagga agcggaggag gaggtagcgg acctaagaaa aagaggaagg
     4081 tggcggccgc tggatcccct tcagggcaga tcagcaacca ggccctggct ctggccccta
     4141 gctccgctcc agtgctggcc cagactatgg tgccctctag tgctatggtg cctctggccc
     4201 agccacctgc tccagcccct gtgctgaccc caggaccacc ccagtcactg agcgctccag
     4261 tgcccaagtc tacacaggcc ggcgagggga ctctgagtga agctctgctg cacctgcagt
     4321 tcgacgctga tgaggacctg ggagctctgc tggggaacag caccgatccc ggagtgttca
     4381 cagatctggc ctccgtggac aactctgagt ttcagcagct gctgaatcag ggcgtgtcca
     4441 tgtctcatag tacagccgaa ccaatgctga tggagtaccc cgaagccatt acccggctgg
     4501 tgaccggcag ccagcggccc cccgaccccg ctccaactcc cctgggaacc agcggcctgc
     4561 ctaatgggct gtccggagat gaagacttct caagcatcgc tgatatggac tttagtgccc
     4621 tgctgtcaca gatttcctct agtgggcagg gaggaggtgg aagcggcttc agcgtggaca
     4681 ccagtgccct gctggacctg ttcagcccct cggtgaccgt gcccgacatg agcctgcctg
     4741 accttgacag cagcctggcc agtatccaag agctcctgtc tccccaggag ccccccaggc
     4801 ctcccgaggc agagaacagc agcccggatt cagggaagca gctggtgcac tacacagcgc
     4861 agccgctgtt cctgctggac cccggctccg tggacaccgg gagcaacgac ctgccggtgc
     4921 tgtttgagct gggagagggc tcctacttct ccgaagggga cggcttcgcc gaggacccca
     4981 ccatctccct gctgacaggc tcggagcctc ccaaagccaa ggaccccact gtctccgcta
     5041 gcggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     5101 gcccaatgcc caagaagaag aggaaggtgt ccaatttact gaccgtacac caaaatttgc
     5161 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca
     5221 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
     5281 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc
     5341 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt
     5401 tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg
     5461 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa
     5521 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata
     5581 gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt
     5641 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga
     5701 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg
     5761 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtatct ggtgtagctg
     5821 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg
     5881 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga
     5941 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
     6001 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
     6061 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
     6121 caggggcaat ggtgcgcctg ctggaagatg gcgattagaa acgttgttgt ttggggttga
     6181 attactctac atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc
     6241 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6301 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6361 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6421 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6481 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6541 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6601 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6661 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6721 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6781 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     6841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6901 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     7141 cccggcgaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7201 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7321 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7381 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     7441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7861 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7981 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8041 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8101 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     8161 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     8221 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     8281 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     8341 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     8401 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     8461 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     8521 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     8581 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     8641 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     8701 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8761 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8821 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8881 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8941 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     9001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9061 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     9121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    10021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    10081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    10141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    11041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    11101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
