LOCUS       sequence-402631-        6887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9)
            fused to V5-tag under the human synapsin (SYN) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Nieuwenhuis, Eva Labs
  TITLE     Plasmid DNA for mammalian expression and viral vectors
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AAV-SYN-integrin alpha9-V5
FEATURES             Location/Qualifiers
     source          1..6887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     protein_bind    148..168
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        190..637
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    637..661
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      662..671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3836..3877
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(3881..3905)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3929..3946)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3935..4142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4150..4290
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4150..4279
                     /label=AAV2 ITR
     rep_origin      4365..4820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4452..4471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4662..4683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4837..4856)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4956..4978
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5016..5034)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5102..5206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5207..5275,5276..6067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5425..5444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6238..6826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6727..6746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagcaa gtttgtacaa aaaagcaggc
      661 tgccaccatg gggagccgga cgccagagtc ccctctccat gccgtgcagc tgcgctgggg
      721 cccccggcgc cgacccccgc tactgccgct gctattgctg ctcgtgccgc cgccaccgcg
      781 ggtcgggggc tacaacctgg atccgcagcg ccccgtgcac ttccagggcc ccgctgactc
      841 gttcttcggc tacgcagttc tggagcattt ccacgacaac acgcgctggg tccttgtggg
      901 cgcaccaaag gcagattcca aatacagccc ttcagtgaag tctcctgggg ctgtgtttaa
      961 gtgccgtgtt cacaccaacc ctgaccggag atgcaccgaa ctggacatgg ctcgagggaa
     1021 gaatcggggc acgtcctgcg gaaagacctg ccgggaagac cgcgatgatg agtggatggg
     1081 ggtgagcctg gcccgacagc ccaaggctga tggccgtgtg ttggcctgtg ctcatcgctg
     1141 gaagaacatc tactatgaag ccgaccacat cctaccccat ggcttctgct acatcatccc
     1201 ctccaacctc caggccaaag gcaggacgct gatcccttgc tatgaagagt ataagaagaa
     1261 gtacggagag gaacacggct cctgccaggc tgggatagcg ggcttcttca ccgaggagct
     1321 ggtggtgatg ggtgctccag ggtcatttta ttgggctgga accatcaaag tgctgaacct
     1381 tacggacaac acctatttaa aactgaacga cgaagtgatt atgaacaggc ggtacaccta
     1441 cctgggctac gcagtgaccg ctggccactt ctctcacccg tccaccattg atgtggtagg
     1501 aggtgcccca caggacaaag gcatcggcaa ggtttatatt ttcagagctg accgaagatc
     1561 aggcacctta attaagatct ttcaagcatc aggtaaaaag atgggctctt acttcggctc
     1621 ctccttgtgc gcagttgacc tgaatgggga cggcctctct gacctgctgg tgggggcccc
     1681 catgttttct gagatcaggg atgagggaca ggtcactgtc tacatcaaca gaggaaatgg
     1741 agccctcgag gagcagctgg ctctgactgg ggatggtgcc tacaatgcgc actttggaga
     1801 gagcattgcc agcctggacg atctggacaa tgatgggttc ccagatgtgg ccattggtgc
     1861 acccaaggag gatgacttcg caggggcggt ctatatctat catggtgatg ccggtgggat
     1921 agtccctcag tactcaatga aactgtctgg gcagaagata aatccagtgc tccggatgtt
     1981 tggtcagtcc atatcgggag gcattgatat ggatggaaat ggctatcctg atgtcactgt
     2041 tggagccttc atgtccgaca gcgtggttct tctcagagca aggcctgtca ttacggtgga
     2101 tgtctccatc ttcctcccgg gctccatcaa catcacagcg cctcagtgtc acgacggaca
     2161 gcagcctgtg aactgcctga acgtcaccac ctgcttcagc ttccatggca aacacgttcc
     2221 agaagagatt ggcctgaatt atgttctgat ggctgacgtg gccaaaaagg agaagggcca
     2281 gatgcccagg gtctactttg tgctgctggg agagaccatg ggtcaggtca cagagaagct
     2341 gcagctgact tacatggagg agacgtgtcg tcactatgtg gcccatgtga agcggagggt
     2401 gcaggacgtc atcagcccga tcgtgtttga agcagcctac agcctcagtg agcatgtgac
     2461 tggagaggag gagagggaac tgccgcctct gacaccagtt ctccgctgga aaaagggaca
     2521 aaagattgcc caaaagaatc agactgtttt tgaaaggaat tgccgttcag aggactgtgc
     2581 cgcagacctg cagcttcagg gtaaactgct gctctccagt atggatgaga aaaccctgta
     2641 tctagctttg ggggctgtga agaacatctc cctaaacatc tctatctcca acctcggaga
     2701 tgatgcctat gatgccaacg tgtccttcaa tgtttcccgg gagctcttct tcatcaacat
     2761 gtggcagaag gaggagatgg gcatctcctg tgagctgctg gaatcggact tcctcaaatg
     2821 cagcgtggga tttcctttca tgaggtcaaa gtcaaagtat gaattcagcg tgatctttga
     2881 tacaagccac ctgtctgggg aagaggaagt tctcagcttc attgttactg ctcagagtgg
     2941 caacacggag cgctctgaat ccctgcatga caacaccctc gtgctgatgg tgccactgat
     3001 gcacgaggtg gacacgtcca tcaccggaat catgtctcca acctcctttg tatatggcga
     3061 gtccgtggac gcagccaact tcattcagct ggatgacctg gagtgtcact ttcagcccat
     3121 caatatcacc cttcaggtct acaacactgg cccaagcacc cttccagggt catctgtcag
     3181 catctctttc cctaatcgac tctcatctgg tggtgcagag atgtttcatg tccaggaaat
     3241 ggtggtgggc caagagaagg gaaactgctc tttccagaaa aacccaactc cctgcatcat
     3301 ccctcaagaa caagaaaata tcttccacac aatatttgct tttttcacaa agtctggaag
     3361 aaaagtcttg gactgtgaaa aaccaggaat ttcttgccta acagcacact gtaactttag
     3421 tgctcttgct aaagaagaaa gtcgtactat agacatttac atgctgctga acacagaaat
     3481 actgaaaaag gacagttcgt ctgtcatcca gttcatgtcc cgcgccaagg tgaaggtgga
     3541 tcctgcccta agggtggtgg aaatagctca tgggaaccca gaagaggtga cggtggtctt
     3601 cgaggccctg cacaatctgg agccccgtgg ctacgtcgtg gggtggatca tcgccatcag
     3661 tttgttggtg ggaatcctca tcttcctgct gctggccgtg ctgctctgga agatgggctt
     3721 ctttcgccga aggtacaaag aaattatcga ggctgagaag aaccggaaag agaatgaaga
     3781 cagttgggac tgggtccaga aaaaccaggt accgcgggcc cgggatccac cggtcggtaa
     3841 gcctatccct aaccctctcc tcggtctcga ttctacgtaa acccagcttt cttgtacaaa
     3901 gtgggaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3961 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4021 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4081 ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg
     4141 gagggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4201 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     4261 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4321 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4441 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4561 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4621 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4681 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4741 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4801 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     4861 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4921 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4981 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5041 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5101 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5161 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5221 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5281 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5341 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5401 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5461 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5521 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5581 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5641 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5701 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5761 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5821 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5881 tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc
     5941 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6001 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6061 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6121 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6181 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6241 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6301 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6361 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6421 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6481 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6601 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6721 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6781 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6841 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
