LOCUS       sequence-402650-        4291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Fujinaga K, Cujec TP, Peng J, Garriga J, Price DH, Grana X, Peterlin
            BM
  TITLE     The ability of positive transcription elongation factor B to 
            transactivate human immunodeficiency virus transcription depends on 
            a functional kinase domain, cyclin T1, and Tat.
  JOURNAL   J Virol. 1998 Sep . 72(9):7154-9.
  PUBMED    9696809
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Sep . 72(9):7154-9."
COMMENT     Sequence Label: pSV CDK9 HA (P#598)
FEATURES             Location/Qualifiers
     source          1..4291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        161..179
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     161..178
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1327..1353
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(1415..1434)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1416..1434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1821..1955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1858..1877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1912..1931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2021..2038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2192..2780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2272..2291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2951..3742,3743..3811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3574..3593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3812..3916)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3984..4002
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(4029..4034,4035..4052,4053..4059)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    4067..4083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4072..4094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(4113..4133)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(4118..4291,1..155)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
       61 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      121 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct atttaggtga cactatagaa
      181 ggtacgcctg caggtaccgg tccggaattc atggcaaagc agtacgactc ggtggagtgc
      241 cctttttgtg atgaagtttc caaatacgag aagctcgcca agatcggcca aggcaccttc
      301 ggggaggtgt tcaaggccag gcaccgcaag accggccaga aggtggctct gaagaaggtg
      361 ctgatggaaa acgagaagga ggggttcccc attacagcct tgcgggagat caagatcctt
      421 cagcttctaa aacacgagaa tgtggtcaac ttgattgaga tttgtcgaac caaagcttcc
      481 ccctataacc gctgcaaggg tagtatatac ctggtgttcg acttctgcga gcatgacctt
      541 gctgggctgt tgagcaatgt tttggtcaag ttcacgctgt ctgagatcaa gagggtgatg
      601 cagatgctgc ttaacggcct ctactacatc cacagaaaca agatcctgca tagggacatg
      661 aaggctgcta atgtgcttat cactcgtgat ggggtcctga agctggcaga ctttgggctg
      721 gcccgggcct tcagcctggc caagaacagc cagcccaacc gctacaccaa ccgtgtggtg
      781 acactctggt accggccccc ggagctgttg ctcggggagc gggactacgg cccccccatt
      841 gacctgtggg gtgctgggtg catcatggca gagatgtgga cccgcagccc catcatgcag
      901 ggcaacacgg agcagcacca actcgccctc atcagtcagc tctgcggctc catcacccct
      961 gaggtgtggc caaacgtgga caactatgag ctgtacgaaa agctggagct ggtcaagggc
     1021 cagaagcgga aggtgaagga caggctgaag gcctatgtgc gtgacccata cgcactggac
     1081 ctcatcgaca agctgctggt gctggaccct gcccagcgca tcgacagcga tgacgccctc
     1141 aaccacgact tcttctggtc cgaccccatg ccctccgacc tcaagggcat gctctccacc
     1201 cacctgacgt ccatgttcga gtacttggca ccaccgcgcc ggaagggcag ccagatcacc
     1261 cagcagtcca ccaaccagag tcgcaatccc gccaccacca accagacgga gtttgagcgc
     1321 gtcttctatg atgttcctga ttatgctagt ctctaggcgg ccgctctaga ggatccaagc
     1381 ttacgtacgc gtgcatgcga cgtcatagct ctctccctat agtgagtcgt attataagct
     1441 agcttgggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     1501 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     1561 tagctgcata tgcttgctgc ttgagagttt tgcttactga gtatgattta tgaaaatatt
     1621 atacacagga gctagtgatt ctaattgttt gtgtatttta gattcacagt cccaaggctc
     1681 atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg
     1741 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1801 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1861 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1921 ccaaactcat caatgtatct tatcatgtct ggatcctgca ttaatgaatc ggccaacgcg
     1981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3961 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcac tcattaggca
     4021 ccccaggctt tacactttat gcttccggct cgtataatgt gtggaattgt gagcggataa
     4081 caatttcaca caggaaacag catcgatgct gtggaatgtg tgtcagttag ggtgtggaaa
     4141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4201 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4261 tagtcagcaa ccatagtccc gcccctaact c
//
