LOCUS       sequence-402655-        6914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-Spike
FEATURES             Location/Qualifiers
     source          1..6914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4225..4324)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4341..4360)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4342..4360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4365..4381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4365..4381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4812..5603
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5696..6284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6185..6204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6438..6455
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6614..6641)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6733..6819)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6868..6890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6882..6899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6883..6899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct tattaattat ttttattttg cctacaacat tagctgttat aggtgatttt
      181 aattgtacta attttgctat taatgattta aacaccacag ttcctcgcat aagtgagtat
      241 gttgtggatg tttcttatgg tttgggtaca tattatatac ttgatcgtgt ttatttaaat
      301 actactatat tatttactgg ttatttccct aaatctggtg ccaattttag ggatctatct
      361 ttaaaaggta ctacatattt gagtactctt tggtatcaga aacccttttt atctgatttt
      421 aataatggta ttttttctag agttaagaat actaagttgt atgttaataa aactttgtat
      481 agtgagttta gtactatagt tataggtagt gtttttatta acaactctta tactattgtt
      541 gttcaacctc ataatggtgt tttggagatt acagcttgtc aatacactat gtgtgagtat
      601 cctcatacta tttgtaaatc taaaggtagt tctcgtaatg aatcttggca ttttgataaa
      661 tctgaacctt tgtgtctgtt caagaaaaat tttacttata atgtttctac agattggttg
      721 tattttcatt tttatcaaga acgtggcact ttttatgctt attatgctga ttctggcatg
      781 cctactactt ttttatttag tttgtatctt ggtactcttt tatctcatta ttatgttttg
      841 cctttgactt gtaatgctat atcttctaat actgataatg agactttaca atattgggtc
      901 acacctttgt ctaaacgcca atatcttctt aaatttgaca accgtggtgt tattactaat
      961 gctgttgatt gttctagtag tttctttagc gagattcaat gtaaaactaa atctttatta
     1021 cctaatactg gtgtttatga cttatctggt tttactgtta agcctgttgc aactgtacat
     1081 cgtcgtattc ctgatttacc tgattgtgac attgataaat ggcttaacaa ttttaatgta
     1141 ccctcacctc ttaattggga acgtaaaatt ttttctaatt gcaactttaa tttgagtact
     1201 ttgcttcgtt tagttcatac tgattctttt tcttgtaata attttgatga atctaagata
     1261 tatggtagtt gttttaagag tattgtttta gataaatttg ccatacccaa ctccagacga
     1321 tctgatttgc agttgggcag ttctggtttt ctgcaatctt ctaattataa aattgacact
     1381 acttctagtt cttgtcaatt gtattatagt ttgcctgcaa ttaatgttac tattaataat
     1441 tataatcctt cttcttggaa tagaaggtat ggttttaata attttaattt gagctctcat
     1501 agtgttgttt actcacgtta ttgtttttct gttaataata ctttttgtcc ttgtgctaaa
     1561 ccttcttttg cttcaagttg caagagtcat aaaccacctt ctgcttcctg tcctattggt
     1621 actaattatc gttcttgtga gagtactact gtactcgacc acactgactg gtgtaggtgt
     1681 tcttgtttac ctgatcctat aactgcttat gaccctaggt cttgttctca aaaaaagtct
     1741 ctggttggtg ttggtgaaca ttgtgcaggg ttcggtgttg atgaagaaaa gtgtggtgta
     1801 ttggatggat catataatgt ttcttgtctt tgtagtactg atgcctttct aggttggtct
     1861 tatgacactt gcgtcagtaa caaccgttgt aatatttttt ctaattttat tttaaatggt
     1921 atcaatagtg gtaccacttg ttctaatgat ttattgcagc ctaatactga agtttttact
     1981 gatgtttgtg ttgattacga cctttatggt attacaggac aaggtatttt taaagaagtt
     2041 tctgctgttt attataatag ttggcaaaat cttttgtatg attctaatgg caacattatt
     2101 ggttttaaag attttgttac taataaaaca tataatattt tcccttgtta tgcaggaaga
     2161 gtttctgctg cttttcatca aaatgcttcc tctttggctt tactttatcg taatttaaaa
     2221 tgtagctatg ttttgaataa tatttcttta actactcagc catattttga tagttatctt
     2281 ggttgcgttt ttaatgctga taatttaact gattattctg tttcttcttg tgctcttcgc
     2341 atgggtagtg gtttttgtgt tgattataac tcaccttctt cttcctcttc gcgtcgtaaa
     2401 cgtagaagta tttctgcttc ttatcgtttt gttacttttg aaccctttaa tgtcagtttt
     2461 gttaatgaca gtattgagtc tgtgggtggt ctttatgaga tcaaaattcc cactaacttt
     2521 actatagttg gtcaagagga atttattcaa actaattctc ctaaagttac tattgattgt
     2581 tctttatttg tctgttctaa ttatgcagct tgccatgact tattgtcaga gtatggcact
     2641 ttttgtgata atattaatag tattttagat gaagttaatg gtttacttga tactactcaa
     2701 ttgcatgtag ctgatactct tatgcaaggt gtcacactta gctccaatct taatactaat
     2761 ttgcattttg atgttgataa tattaatttt aaatccctag ttggatgttt aggtccacac
     2821 tgcggttctt cttctcgttc tttttttgaa gatttattgt ttgacaaagt taaactttca
     2881 gatgttggtt ttgttgaagc ttataacaat tgtactggtg gtagtgaaat tagagatctt
     2941 ctttgtgtac aatcctttaa tggtattaaa gttttgcctc ctattttgtc tgaatctcaa
     3001 atttctggtt acaccacagc cgctactgtt gctgctatgt ttccaccatg gtcagcagca
     3061 gctggcatac cattttctct taatgtacaa tatagaatta atggtttggg tgttactatg
     3121 gatgttctta ataaaaatca aaagttgata gctactgctt ttaataatgc tcttctttct
     3181 attcagaatg gttttagtgc taccaactct gcacttgcta aaatacaaag tgttgttaat
     3241 tctaatgctc aagcacttaa tagtttgtta cagcaattat ttaataaatt tggtgcaatt
     3301 agttcttctt tacaagaaat tttatctcgt ctcgatgctt tagaggctca ggttcagatt
     3361 gataggctta ttaatggtcg tttaactgct ttaaatgctt atgtctctca acagcttagt
     3421 gatatttctc ttgtaaaatt tggtgctgct ttagctatgg agaaggttaa tgagtgtgtt
     3481 aaaagtcaat ctcctcgtat taatttttgt ggtaatggta atcatatttt gtcattagtt
     3541 caaaatgctc cttatggttt gttgtttatg cattttagtt ataaacctat ttcttttaaa
     3601 actgttttag taagtcctgg tttgtgtata tcaggtgatg taggtattgc acctaaacaa
     3661 gggtatttta ttaaacataa tgatcattgg atgttcactg gtagttctta ctattatcct
     3721 gaaccaattt cagataaaaa tgttgttttt atgaatactt gttctgttaa ttttactaaa
     3781 gcgcctcttg tttatttgaa tcattctgta ccaaaattgt ctgattttga atctgagtta
     3841 tctcattggt ttaaaaatca aacatccatt gcgcctaatt tgactttaaa tcttcatact
     3901 attaatgcta cttttttaga tttgtattat gagatgaatc ttattcaaga gtctattaag
     3961 tctttgaata atagttatat caatcttaaa gatataggta catatgaaat gtatgtaaaa
     4021 tggccttggt atgtttggct actaatttct ttttcattta taatattcct tgtattgctc
     4081 ttttttatat gttgttgtac tggttgtggt tctgcatgtt ttagtaaatg tcataattgt
     4141 tgtgatgagt atggtggtca tcatgatttt gttatcaaaa catctcatga tgatcttaat
     4201 taagacgcga tcgcggcctc atgggcccag ctttcttgta caaagttggc attataaaaa
     4261 ataattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     4321 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     4381 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     4441 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     4501 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     4561 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     4621 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     4681 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     4741 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     4801 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     4861 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4921 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     4981 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     5041 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     5101 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     5161 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     5221 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     5281 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     5341 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     5401 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     5461 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     5521 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     5581 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     5641 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5701 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5761 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5821 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5881 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5941 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6001 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6061 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6121 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6181 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6241 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6301 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6361 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6421 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6481 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6541 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     6601 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     6661 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     6721 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     6781 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     6841 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     6901 ttaagctcgg gccc
//
