LOCUS       sequence-402656-        6914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-NL63-Spike
FEATURES             Location/Qualifiers
     source          1..6914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4225..4324)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4341..4360)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4342..4360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4365..4381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4365..4381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4812..5603
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5696..6284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6185..6204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6438..6455
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6614..6641)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6733..6819)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6868..6890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6882..6899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6883..6899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcca aacttttctt gattttgctt gttttgcccc tggcctcttg ctttttcaca
      181 tgtaatagta atgctaatct ctctatgtta caattaggtg ttcctgacaa ttcttcaact
      241 attgttacgg gtttattgcc aactcattgg ttttgtgcta atcagagtac atctgtttac
      301 tcagccaatg gtttctttta tattgatgtt ggtaatcacc gtagtgcttt tgcgctccat
      361 actggttatt atgatgctaa tcagtattat atttatgtta ctaatgaaat aggcttaaat
      421 gcttctgtta ctcttaagat ttgtaagttt agtagaaaca ctacttttga ttttttaagt
      481 aatgcttcta gttcttttga ctgtatagtt aatttgttat ttacagaaca gttaggtgcg
      541 cctttgggca taactatatc tggtgaaact gtgcgtctgc atttatataa tgtaactcgt
      601 actttttatg tgccagcagc ttataaactt actaaactta gtgttaaatg ttactttaac
      661 tattcctgtg tttttagtgt tgtcaacgcc accgttactg tgaatgtcac cacacataat
      721 ggccgtgtag ttaactacac tgtttgtgat gattgtaatg gttatactga taacatattt
      781 tctgttcaac aggatggccg cattcctaat ggtttccctt ttaataattg gtttttgtta
      841 actaatggtt ccacactagt ggacggggtc tctagacttt atcaaccact ccgtttaact
      901 tgtttatggc ctgtacctgg tcttaaatct tcaactggtt ttgtttattt taatgccact
      961 ggttctgatg ttaattgtaa cggctatcaa cataattctg ttgttgatgt tatgcgttac
     1021 aatcttaact tcagtgctaa ttctttggac aatctcaaga gtggtgttat agtttttaaa
     1081 actttacagt acgatgtttt gttttattgt agtaattctt cctcaggtgt tcttgacacc
     1141 acaatacctt ttggcccgtc ctctcaacct tattactgtt ttataaacag cactatcaac
     1201 actactcatg ttagcacttt tgtgggtatt ttaccaccca ctgtgcgtga aattgttgtt
     1261 gctagaactg gccagtttta tattaatggt tttaagtatt tcgatttggg tttcatagaa
     1321 gctgtcaatt ttaatgtcac gactgctagc gccacagatt tttggacggt tgcatttgct
     1381 acttttgttg atgttttggt taatgttagt gcaactaaca ttcaaaactt actttattgc
     1441 gattctccat ttgaaaagtt gcagtgtgag cacttgcagt ttggattgca ggatggtttt
     1501 tattctgcaa attttcttga tgataatgtt ttgcctgaga cttatgttgc actccccatt
     1561 tattatcaac acacggacat aaattttact gcaactgcat cttttggtgg ttcttgttat
     1621 gtttgtaaac cacaccaggt taatatatct cttaatggta acacttcagt gtgtgttaga
     1681 acatctcatt tttcaattag gtatatttat aaccgcgtta agagtggttc accaggtgac
     1741 tcttcatggc acatttattt aaagagtggc acttgtccat tttctttttc taagttaaat
     1801 aattttcaaa agttcaagac tatttgtttc tcaaccgtcg aagtgcctgg tagttgtaat
     1861 tttccgcttg aagccacctg gcattacact tcttatacta ttgttggtgc tttgtatgtt
     1921 acttggtctg aaggtaattc tattactggt gtaccttatc ctgtctctgg tattcgtgag
     1981 tttagtaatt tagttttaaa taattgtacc aaatataata tttatgatta tgttggtact
     2041 ggaattatac gttcttcaaa ccagtcactt gctggtggta ttacatatgt ttctaactct
     2101 ggtaatttac ttggttttaa aaatgtttcc actggtaaca tttttattgt gacaccatgt
     2161 aaccaaccag accaagtagc tgtttatcaa caaagcatta ttggtgccat gaccgctgtt
     2221 aatgagtcta gatatggctt gcaaaactta ctacagttac ctaactttta ttatgttagt
     2281 aatggtggta acaattgcac tacggccgtt atgacttatt ctaattttgg tatttgtgct
     2341 gatggttctt tgattcctgt tcgtccgcgt aattctagtg ataatggtat ttcagccata
     2401 atcactgcta atttatccat tccttctaac tggactactt cagttcaagt tgagtacctc
     2461 caaattacta gtactccaat agttgttgat tgtgctactt atgtgtgtaa tggtaaccct
     2521 cgctgtaaga atctacttaa gcagtatact tctgcttgta aaactattga agatgcctta
     2581 cgacttagtg ctcatttgga aactaatgat gttagtagta tgctaacttt cgatagcaat
     2641 gcttttagtt tggctaatgt tactagtttt ggagattata acctttctag tgttttacct
     2701 cagagaaaca ttcgttcaag ccgtatagca ggacgtagtg ctttggaaga tttgttgttt
     2761 agcaaagttg ttacatctgg tttgggtact gttgatgttg actataagtc ttgtactaaa
     2821 ggtctttcta ttgctgacct tgcttgtgct cagtactaca atggcataat ggttttgcca
     2881 ggtgttgctg atgctgaacg tatggccatg tacacaggtt ctcttatagg tggcatggtg
     2941 ctcggaggtc ttacatcagc agccgccata cctttttctt tggcactgca agcacgactt
     3001 aactatgttg ctttacaaac tgatgtgctt caagaaaatc agaaaatttt ggctgcatca
     3061 tttaataagg ctattaataa tattgttgct tcttttagta gcgttaatga tgctattaca
     3121 caaactgcag aggctataca tactgttact attgcactta ataagattca ggatgttgtt
     3181 aatcaacagg gtagtgctct taaccatctc acttcacaat tgagacataa ttttcaggcc
     3241 atttctaatt caattcaggc tatttatgac cggcttgatt caattcaagc cgatcaacaa
     3301 gttgacagat taattactgg acggcttgca gctttgaatg catttgtttc ccaagttttg
     3361 aataaatata ctgaagttcg tggttcaaga cgcttagcac agcagaagat taatgaatgt
     3421 gtcaagtcac aatctaatag atatggtttt tgtggcaatg gcactcacat cttttcaatc
     3481 gtcaactcag ctccagatgg tttgcttttt cttcatactg ttttgctgcc aactgattac
     3541 aagaatgtaa aggcgtggtc tggtatctgt gttgatggca tttatggcta tgttctgcgt
     3601 caacctaact tggttcttta ttctgataat ggtgtctttc gtgtaacttc cagggtcatg
     3661 tttcaacctc gcttacctgt tttgtctgat tttgtgcaaa tatataattg taatgttact
     3721 tttgttaaca tatctcgtgt tgagttacat actgtcatac ctgactacgt tgatgttaat
     3781 aaaacattac aagagtttgc acaaaactta ccaaagtatg ttaagcctaa ttttgacttg
     3841 actcctttta atttaacata tcttaatttg agttctgagt tgaagcaact cgaagctaaa
     3901 actgctagtc tttttcaaac tactgttgaa ttacaaggtc ttattgatca gattaacagt
     3961 acatatgttg atttgaagtt gcttaatagg tttgaaaatt atatcaaatg gccttggtgg
     4021 gtttggctca ttatttctgt tgtttttgtt gtattgttga gtcttcttgt gttttgttgt
     4081 ctttctacag gttgttgtgg ttgttgcaat tgtttaactt catcaatgcg aggctgttgt
     4141 gattgtggtt caactaaact tccttattac gaatttgaaa aggtccacgt tcaacttaat
     4201 taagacgcga tcgcggcctc atgggcccag ctttcttgta caaagttggc attataaaaa
     4261 ataattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     4321 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     4381 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     4441 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     4501 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     4561 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     4621 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     4681 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     4741 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     4801 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     4861 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4921 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     4981 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     5041 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     5101 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     5161 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     5221 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     5281 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     5341 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     5401 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     5461 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     5521 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     5581 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     5641 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5701 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5761 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5821 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5881 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5941 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6001 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6061 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6121 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6181 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6241 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6301 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6361 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6421 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6481 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6541 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     6601 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     6661 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     6721 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     6781 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     6841 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     6901 ttaagctcgg gccc
//
