LOCUS       sequence-402657-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-Spike
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4216..4315)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4332..4351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4333..4351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4356..4372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4356..4372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4803..5594
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5687..6275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6176..6195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6429..6446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6605..6632)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6724..6810)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6859..6881
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6873..6890
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6874..6890
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ttttgatact tttaatttcc ttaccaacgg cttttgctgt tataggagat
      181 ttaaagtgta cttcagataa tattaatgat aaagacaccg gtcctcctcc tataagtact
      241 gatactgttg atgttactaa tggtttgggt acttattatg ttttagatcg tgtgtattta
      301 aatactacgt tgtttcttaa tggttattac cctacttcag gttccacata tcgtaatatg
      361 gcactgaagg gaagtgtact attgagcaga ctatggttta aaccaccatt tctttctgat
      421 tttattaatg gtatttttgc taaggtcaaa aataccaagg ttattaaaga tcgtgtaatg
      481 tatagtgagt tccctgctat aactataggt agtacttttg taaatacatc ctatagtgtg
      541 gtagtacaac cacgtacaat caattcaaca caggatggtg ataataaatt acaaggtctt
      601 ttagaggtct ctgtttgcca gtataatatg tgcgagtacc cacaaacgat ttgtcatcct
      661 aacctgggta atcatcgcaa agaactatgg catttggata caggtgttgt ttcctgttta
      721 tataagcgta atttcacata tgatgtgaat gctgattatt tgtattttca tttttatcaa
      781 gaaggtggta ctttttatgc atattttaca gacactggtg ttgttactaa gtttttgttt
      841 aatgtttatt taggcatggc gctttcacac tattatgtca tgcctctgac ttgtaatagt
      901 aagcttactt tagaatattg ggttacacct ctcacttcta gacaatattt actcgctttc
      961 aatcaagatg gtattatttt taatgctgtt gattgtatga gtgattttat gagtgagatt
     1021 aagtgtaaaa cacaatctat agcaccacct actggtgttt atgaattaaa cggttacact
     1081 gttcagccaa tcgcagatgt ttaccgacgt aaacctaatc ttcccaattg caatatagaa
     1141 gcttggctta atgataagtc ggtgccctct ccattaaatt gggaacgtaa gacattttca
     1201 aattgtaatt ttaatatgag cagcctgatg tcttttattc aggcagactc atttacttgt
     1261 aataatattg atgctgctaa gatatatggt atgtgttttt ccagcataac tatagataag
     1321 tttgctatac ccaatggcag gaaggttgac ctacaattgg gtaatttggg ctatttgcag
     1381 tcatttaact atagaattga tactactgca acaagttgtc agttgtatta taatttacct
     1441 gctgctaatg tttctgttag caggtttaat ccttctactt ggaataagag atttggtttt
     1501 atagaagatt ctgtttttaa gcctcgacct gcaggtgttc ttactaatca tgatgtagtt
     1561 tatgcacaac actgtttcaa agctcctaaa aatttctgtc cgtgtaaatt gaatggttcg
     1621 tgtgtaggta gtggtcctgg taaaaataat ggtataggca cttgtcctgc aggtactaat
     1681 tatttaactt gtgataattt gtgcactcct gatcctatta catttacagg tacttataag
     1741 tgcccccaaa ctaaatcttt agttggcata ggtgagcact gttcgggtct tgctgttaaa
     1801 agtgattatt gtggaggcaa ttcttgtact tgccgaccac aagcattttt gggttggtct
     1861 gcagactctt gtttacaagg agacaagtgt aatatttttg ctaattttat tttgcatgat
     1921 gttaatagtg gtcttacttg ttctactgat ttacaaaaag ctaacacaga cataattctt
     1981 ggtgtttgtg ttaattatga cctctatggt attttaggcc aaggcatttt tgttgaggtt
     2041 aatgcgactt attataatag ttggcagaac cttttatatg attctaatgg taatctctac
     2101 ggttttagag actacataac aaacagaact tttatgattc gtagttgcta tagcggtcgt
     2161 gtttctgcgg cctttcacgc taactcttcc gaaccagcat tgctatttcg gaatattaaa
     2221 tgcaactacg tttttaataa tagtcttaca cgacagctgc aacccattaa ctattttgat
     2281 agttatcttg gttgtgttgt caatgcttat aatagtactg ctatttctgt tcaaacatgt
     2341 gatctcacag taggtagtgg ttactgtgtg gattactcta aaaacagacg aagtcgtgga
     2401 gcgattacca ctggttatcg gtttactaat tttgagccat ttactgttaa ttcagtaaac
     2461 gatagtttag aacctgtagg tggtttgtat gaaattcaaa taccttcaga gtttactata
     2521 ggtaatatgg tggagtttat tcaaacaagc tctcctaaag ttactattga ttgtgctgca
     2581 tttgtctgtg gtgattatgc agcatgtaaa tcacagttgg ttgaatatgg tagtttctgt
     2641 gataacatta atgccatact cacagaagta aatgaactac ttgacactac acagttgcaa
     2701 gtagctaata gtttaatgaa tggtgttact cttagcacta agcttaaaga tggcgttaat
     2761 ttcaatgtag acgacatcaa tttttcccct gtattaggtt gtctaggcag cgaatgtagt
     2821 aaagcttcca gtagatctgc tatagaggat ttactttttg ataaagtaaa gttatctgat
     2881 gtcggttttg ttgaggctta taataattgt acaggaggtg ccgaaattag ggacctcatt
     2941 tgtgtgcaaa gttataaagg catcaaagtg ttgcctccac tgctctcaga aaatcagatc
     3001 agtggataca ctttggctgc cacctctgct agtctatttc ctccttggac agcagcagca
     3061 ggtgtaccat tttatttaaa tgttcagtat cgcattaatg ggcttggtgt caccatggat
     3121 gtgctaagtc aaaatcaaaa gcttattgct aatgcattta acaatgccct ttatgctatt
     3181 caggaagggt tcgatgcaac taattctgct ttagttaaaa ttcaagctgt tgttaatgca
     3241 aatgctgaag ctcttaataa cttattgcaa caactctcta atagatttgg tgctataagt
     3301 gcttctttac aagaaattct atctagactt gatgctcttg aagcggaagc tcagatagat
     3361 agacttatta atggtcgtct taccgctctt aatgcttatg tttctcaaca gcttagtgat
     3421 tctacactgg taaaatttag tgcagcacaa gctatggaga aggttaatga atgtgtcaaa
     3481 agccaatcat ctaggataaa tttctgtggt aatggtaatc atattatatc attagtgcag
     3541 aatgctccat atggtttgta ttttatccac tttagttatg tccctactaa gtatgtcaca
     3601 gcgagggtta gtcctggtct gtgcattgct ggtgatagag gtatagctcc taagagtggt
     3661 tattttgtta atgtaaataa tacttggatg tacactggta gtggttacta ctaccctgaa
     3721 cctataactg aaaataatgt tgttgttatg agtacctgcg ctgttaatta tactaaagcg
     3781 ccgtatgtaa tgctgaacac ttcaataccc aaccttcctg attttaagga agagttggat
     3841 caatggttta aaaatcaaac atcagtggca ccagatttgt cacttgatta tataaatgtt
     3901 acattcttgg acctacaagt tgaaatgaat aggttacagg aggcaataaa agtcttaaat
     3961 cagagctaca tcaatctcaa ggacattggt acatatgaat attatgtaaa atggccttgg
     4021 tatgtatggc ttttaatctg ccttgctggt gtagctatgc ttgttttact attcttcata
     4081 tgctgttgta caggatgtgg gactagttgt tttaagaaat gtggtggttg ttgtgatgat
     4141 tatactggat accaggagtt agtaatcaaa acttcacatg acgaccttaa ttaagacgcg
     4201 atcgcggcct catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct
     4261 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     4321 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     4381 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     4441 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     4501 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     4561 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     4621 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     4681 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     4741 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     4801 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     4861 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     4921 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     4981 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     5041 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     5101 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     5161 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     5221 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     5281 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     5341 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     5401 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     5461 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     5521 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     5581 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     5641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5821 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6061 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6481 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6541 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     6601 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     6661 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     6721 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     6781 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     6841 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     6901 ggccc
//
