LOCUS       sequence-402658-        6365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-Spike
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3676..3775)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3792..3811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3793..3811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3816..3832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3816..3832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4263..5054
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5147..5735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5636..5655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5889..5906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6065..6092)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6184..6270)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6319..6341
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6333..6350
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6334..6350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ttgttttgct tgttgcatat gccttgttgc atattgctgg ttgtcaaact
      181 acaaatgggc tgaacactag ttactctgtt tgcaacggct gtgttggtta ttcagaaaat
      241 gtatttgctg ttgagagtgg tggttatata ccctccgact ttgcattcaa taattggttc
      301 cttctaacta atacctcatc tgttgtagat ggtgttgtga ggagttttca gcctttgttg
      361 cttaattgct tatggtctgt ttctggcttg cggtttacta ctggttttgt ctattttaat
      421 ggtactggga gaggtgattg taaaggtttt tcctcagatg ttttgtctga tgtcatacgt
      481 tacaacctca attttgaaga aaaccttaga cgtggaacca ttttgtttaa aacatcttat
      541 ggtgttgttg tgttttattg taccaacaac actttagttt caggtgatgc tcacatacca
      601 tttggtacag ttttgggcaa tttttattgc tttgtaaata ctactattgg caatgaaact
      661 acgtctgctt ttgtgggtgc actacctaag acagttcgtg agtttgttat ttcacgcaca
      721 ggacattttt atattaatgg ctatcgctat ttcactttag gtaatgtaga agccgttaat
      781 ttcaatgtca ctactgcaga aaccactgat ttttgtactg ttgcgttagc ttcttatgct
      841 gacgttttgg ttaatgtgtc acaaacctct attgctaata taatttattg caactctgtt
      901 attaacagac tgagatgtga ccagttgtcc tttgatgtac cagatggttt ttattctaca
      961 agccctattc aatccgttga gctacctgtg tctattgtgt cgctacctgt ttatcataaa
     1021 catacgttta ttgtgttgta cgttgacttc aaacctcaga gtggcggtgg caagtgcttt
     1081 aactgttatc ctgctggtgt taatattaca ctggccaatt ttaatgaaac taaagggcct
     1141 ttgtgtgttg acacatcaca cttcactacc aaatacgttg ctgtttatgc caatgttggt
     1201 aggtggagtg ctagtattaa cacgggaaat tgcccttttt cttttggcaa agttaataac
     1261 tttgttaaat ttggcagtgt atgtttttcg ctaaaggata tacccggtgg ttgcgcaatg
     1321 cctatagtgg ctaattgggc ttatagtaag tactatacta taggctcatt gtatgtttct
     1381 tggagtgatg gtgatggaat tactggcgtc ccacaacctg ttgagggtgt tagttccttt
     1441 atgaatgtta cattggacaa atgtactaaa tataatattt atgatgtatc tggtgtgggt
     1501 gttattcgcg ttagcaatga cacctttctt aatggaatta cgtacacatc aacttcaggt
     1561 aaccttctgg gttttaaaga tgttactaag ggcaccatct actctatcac tccttgtaac
     1621 ccaccagatc agcttgttgt ttatcagcaa gctgttgttg gtgctatgtt gtctgaaaat
     1681 tttactagtt acggcttttc taatgttgta gaactgccga aatttttcta tgcgtccaat
     1741 ggcacttata attgcacaga cgctgtttta acttattcta gttttggcgt ttgtgcagat
     1801 ggttctataa ttgctgttca accacgtaat gtttcatatg atagtgtttc agctatcgtc
     1861 acagctaatt tgtctatacc ttccaattgg accacttcgg tccaggttga gtatttacaa
     1921 attacaagta cacctatcgt agttgattgc tccacttatg tttgcaatgg taatgtgcgc
     1981 tgtgttgaat tgcttaagca gtatacttct gcttgtaaaa ctattgaaga cgccttaaga
     2041 aatagcgcca ggctggagtc tgcagatgtt agtgagatgc tcacttttga caagaaagcg
     2101 tttacacttg ctaatgttag tagttttggt gactacaacc ttagcagcgt catacctagc
     2161 ttgcccacaa gtggtagtag agtggctggt cgcagtgcca tagaagacat actttttagc
     2221 aaacttgtta cttctggact tggcactgtg gacgcagact acaaaaagtg cactaagggt
     2281 ctttccattg ctgacttggc ttgtgctcaa tattataatg gcattatggt tttgcctggc
     2341 gtcgctgatg ctgaacgaat ggccatgtat acaggttctt taattggtgg aattgcttta
     2401 ggaggtctaa catcagccgt ttcaatacca ttttcattag caattcaggc acgtttaaat
     2461 tatgttgcat tgcagactga tgttttacaa gaaaatcaga aaattcttgc tgcatctttt
     2521 aacaaagcaa tgaccaacat agtagatgcc tttactggtg ttaatgatgc tattacacaa
     2581 acttcacaag ccctacaaac agttgctact gcacttaaca agatccagga tgttgttaat
     2641 caacaaggca actcattgaa ccatttaact tctcagttga ggcagaattt tcaagctatc
     2701 tctagctcta ttcaggctat ctatgacaga cttgacacta ttcaggctga tcaacaagta
     2761 gataggctga ttactggtag attggctgct ttgaatgtat tcgtttctca tacattgact
     2821 aagtacactg aagttcgtgc ttccagacag cttgcacaac aaaaagtgaa tgagtgtgtc
     2881 aaatcccagt ctaagcgtta tggcttctgt ggaaatggca ctcacatttt ctcaattgtt
     2941 aatgctgctc ctgaggggct tgtttttctc cacactgtct tgttgccgac acaatataag
     3001 gatgttgaag cgtggtctgg gttgtgcgtt gatggtacaa acggttatgt gttgcgacaa
     3061 cctaatcttg ctctttacaa agaaggcaat tattatagaa tcacatctcg cataatgttt
     3121 gaaccacgta ttcctaccat ggcagatttt gttcaaattg aaaattgcaa tgtcacattt
     3181 gttaacattt ctcgctctga gttgcaaacc attgtgccag agtatattga tgttaataag
     3241 acgctgcaag aattaagtta caaattgcca aattacactg ttccagacct agttgtcgaa
     3301 cagtacaacc agactatttt gaatttgacc agtgaaatta gcacccttga aaataaatct
     3361 gcggagctta attacactgt tcaaaaattg caaactctga ttgacaacat aaatagcaca
     3421 ttagtcgact taaagtggct caaccgggtt gagacttaca tcaagtggcc gtggtgggtg
     3481 tggttgtgca tttcagtcgt gctcatcttt gtggtgagta tgttgctatt atgttgttgt
     3541 tctactggtt gctgtggctt ctttagttgt tttgcatctt ctattagagg ttgttgtgaa
     3601 tcaactaaac ttccttatta cgacgttgaa aagatccaca tacagcttaa ttaagacgcg
     3661 atcgcggcct catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct
     3721 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     3781 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     3841 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     3901 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     3961 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     4021 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     4081 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     4141 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     4201 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     4261 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     4321 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     4381 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     4441 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     4501 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     4561 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     4621 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     4681 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     4741 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     4801 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     4861 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     4921 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     4981 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     5041 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     5101 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5281 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5521 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5761 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5821 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5881 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     5941 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6001 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     6061 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     6121 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     6181 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     6241 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     6301 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     6361 ggccc
//
