LOCUS       sequence-402659-        9251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with 
            the tissue-specific promoter semo-1 in C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W
  TITLE     Enhanced single RNA imaging reveals dynamic gene expression in live 
            animals.
  JOURNAL   Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178.
  PUBMED    36867026
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-03-3"; volume: "12:e82178. doi"; pages: " 
            10.7554/eLife.82178"
COMMENT     Sequence Label: pSX2719
FEATURES             Location/Qualifiers
     source          1..9251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3876..3900
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3904..4290
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4312..5016
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4454..4472)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4722..4741
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5035..5091
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5107..5163
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5179..5235
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5251..5307
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5323..5379
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5395..5451
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5467..5523
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5539..5595
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5611..5667
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5683..5739
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5755..5811
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5827..5883
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5899..5955
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5971..6027
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6043..6099
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6115..6171
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6187..6243
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6259..6315
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6331..6387
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6403..6459
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6475..6531
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6547..6603
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6691..6747
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     terminator      complement(6769..7092)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(7102..7118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7102..7118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7115..7137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7126..7142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7150..7156,7157..7174,7175..7180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7195..7216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7333..7350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7504..8092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7584..7603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8263..9054,9055..9123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8886..8905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9124..9228)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 atgctgacaa aagctgggat tggaaataca gaattaaaac taagttgaaa tttgattaaa
      721 tttgccaatt gtctgaaaca gtgtcagtat taattcaaca gttcacagga tgttgctaat
      781 tagcagttag gctaataata actaaagttt tctcaaaagt tgcagaaaaa tatttcactg
      841 tttcactaaa actttattag acgtcgcaat ttaatttata caatgacact tgtttaagcc
      901 aatttccaat attttaaatg cttataacta caggagcatg taacatatcg aatgcaatta
      961 tagtttataa taattttcat attgttctta acattttgtt agttgattat tttttgtatc
     1021 ttcgaagtcc atcaagatgt gaagcttcaa aaacaacaac cgtttgactt aaatacggat
     1081 tcggagtaaa aaaaacgtct taacatcaat ttgggcaagg ttttattttg aatttgacat
     1141 gttcatttca ttagctttca gaaaacatat aattcataac tctctggaca tcaaaaaaag
     1201 agtccctagt tagccttttt aatttgttaa aattataata aaaatcggaa attattgaaa
     1261 tttgaataaa tttaccaatt gtttggaaac gttcctttat tatatacaag ggttaacaaa
     1321 attaaataaa taataaagat aatgcaaaag tctgttgaaa atatttctac atttgggaat
     1381 atatgttgca gtgtttcaat acatttttat taggcggttt acaaatttcc tacggtttac
     1441 aaaaaaatta gtgttaaaga aaaccagtaa aaattgacta ttttaacgat aattgtcaaa
     1501 aattcgaggg ggtgctatag aagttggacg gccgtttttt caactttttg acctaatttc
     1561 cgtagttttt aagatattct cacaaaaacc gaggtgtggc ggttttttaa catttgtaat
     1621 cgaactacat tcacactttt tcaaaataat ttccagaaag cctcaaacac tcaaaataga
     1681 ggggggtgct atagaagttg gacgcactgt atgttgcagt gtttcaatac atttttatta
     1741 ggcggtttac aaatttccca ccgttgcgct tcagttttat gtatttttaa attttcattt
     1801 ctgtctaatt cacagacttt tgcttgcttc actgttttgt atactttttt ttgaaacacc
     1861 gtacttcacc gtgccaaaat ttttgcaaaa aatgttttaa aaaattgtaa ccaaatttgt
     1921 cttgattaga aattttgact tagaaatttt gaaaattgat tgtttttaaa aaatcggaaa
     1981 aattgaaaaa aaccccaata ttaaatttta tgtgaaaaaa acatattaaa agcgctcaac
     2041 ttgcatttca aatcgagact aacttgaaat gtgaataaat ttaccgattg tagaaaaact
     2101 tcaaaactgc aaatttaagc aaaattgcac cagttaacag ttaagctatt aacggcttac
     2161 gtttgcttaa agtttgcaaa aaaatgttta actatttcac taaaacttca ttaggcgttt
     2221 caataaattt tatataatac aatgagaagc aatgtgaaaa tccaataaaa ttcaaatgaa
     2281 agattgataa catgcctcat tttactattg gtatgagttt tctattagta ttgtaacagt
     2341 attaacatgt agcaatatta actgtaattt ccccatatat ttgtggagat ttttatgctt
     2401 aaggtggatt tgaatgactg ttctcatgag gaaatatgaa caacttaata ttcaaatttg
     2461 tttatatttc caataataaa aaaaaaataa aaataaaaaa aatttcgcat gctgaaagac
     2521 gccgaaagtt tttttttcat cgtcgaccaa ttttttttag cgagtgacat ttgccgtttg
     2581 ccgtttgcag cacctaaaat aaataaaaaa taaataaaat tgttgtttaa acctttttat
     2641 tagtggaaaa cgtaataaga aatttgcaaa tttaattaac ccaagctgag tttttctaaa
     2701 aatttgctat attcgcgata cattttttta ctgtttcaag gaatcctcat ggagattttc
     2761 aataatttac agttgatccc atcttcatca ataattttgt ccttcaaatt ttggaatttg
     2821 tcgcgcgcac aaccctgaat aattgtttat tatttttctg ttaagcctag tatcagaaga
     2881 taaattaaaa acgaaaaaac tcactatttt caatttcaaa taaattcacg ttttgtagtc
     2941 agatcggttt tccaaaatta aaaaaaaatg ttatcaaaat atctgtgcac tttgaatcag
     3001 tcacacaatg ttcacaaatt catctctaaa ttcaaaaatt aatctcaacc agaaacggta
     3061 cttgtagttt gtatcagaat aatttcctac gtgtttctag tagatttcta gttggttcat
     3121 tgaacaatca tcttgatgac tgctagagat aagacataga acagctagtg ataccttatc
     3181 aatgtcatcc ccttctgttg tcgattgtct ttttcatttt tgatttttgt gttcagttcg
     3241 ttatataaat agagagtagt ggaaaactga caaagttcct gcaaactaaa cccgtctgag
     3301 ccaaaattaa ttgttttcaa aacagttttg tcacctttcc aaataatcct tttcaaaaac
     3361 aaaattattt tcagtttcac tgtgtgttta taagtttcac atttttcagt tttttttggt
     3421 tttgctacat ccgcgttagt tttttaactt acaaattttc gctgtatttt ttttttaatt
     3481 tttacattcc cctttttaga gctgtgacaa aaaaagtaat tttttctgaa gctaaaaatg
     3541 tttgcccaaa agttttcgtg caacttgttt tcaaagctac aaaaatagtg tcaaacttga
     3601 aaaaaattta attttgctat agtttaattc agcaaaaaat tgtatgtatg taacttttta
     3661 atccatacta ctgatttgat tatattgttt gattttaaaa aaaagtcaat atttgcagat
     3721 caaaaatccc ctcgaggcat gcctgcaggt cgactctaga ggatccccgg gattggccaa
     3781 aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc
     3841 cttggagggt acaggtagaa aaaaagcttg atatcacaag tttgtacaaa aaagcaggct
     3901 atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg cgacgtgact
     3961 gtcgccccaa gcaacttcgc taacggggtc gctgaatgga tcagctctaa ctcgcgttca
     4021 caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg caaatacacc
     4081 atcaaagtcg aggtgcctaa agtggcaacc cagactgttg gtggtgtaga gcttcctgta
     4141 gccgcatggc gttcgtacct aaatatggaa ctaaccattc caattttcgc cacgaattcc
     4201 gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc
     4261 tcagcaatcg cagcaaactc cggcatctac ggatcatcag gtgctggatc cgtgagcaag
     4321 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4381 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4441 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4501 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4561 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4621 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4681 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     4741 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4801 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4861 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4921 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4981 cgccactcca ccggcggcat ggacgagctg tacaagggtg gaggttctgg aggagaagaa
     5041 cttttgagca agaattatca tcttgagaac gaagtggctc gtcttaagaa aggttctggc
     5101 agtggagaag aactgctttc aaagaattac cacctggaaa atgaggtagc tagactgaaa
     5161 aaggggagcg gaagtgggga ggagttgctg agcaaaaatt atcatttgga gaacgaagta
     5221 gcacgactaa agaaagggtc cggatcgggt gaggagttac tctcgaaaaa ttatcatctc
     5281 gaaaacgaag tggctcggct aaaaaagggc agtggttctg gagaagagct attatctaaa
     5341 aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgaagag
     5401 ctactatcca agaattatca tcttgagaac gaggtagcgc gtttgaagaa gggttccggc
     5461 tcaggagagg aactgctctc gaagaactat catcttgaaa atgaggtcgc tcgattaaaa
     5521 aagggatcgg gcagtggtga ggaactactt tcaaagaatt accacctcga aaacgaagta
     5581 gctcgattaa agaaaggttc agggtcgggt gaagaattac tgagtaaaaa ttatcatctg
     5641 gaaaatgagg tagcgagact aaaaaagggg agtggttctg gcgaagagtt gctatcgaaa
     5701 aattatcatc ttgagaacga agttgctagg ctcaaaaagg gctcaggctc aggcgaggag
     5761 ttgctctcga aaaactacca cttggaaaat gaggtcgcga ggttgaaaaa ggggagcggg
     5821 tcgggcgagg agttattgag caaaaactat catttagaga acgaagtcgc gcgcttaaag
     5881 aaaggctcgg gctcgggcga agaactctta tcgaagaact accacctcga aaatgaggtc
     5941 gccaggttga aaaagggcag tggcagcggg gaggaactct tgagcaagaa ctaccacttg
     6001 gagaatgagg tcgcgagatt gaagaaaggg tcggggagcg gcgaggaatt gctcagcaag
     6061 aattatcatt tggagaacga agtcgccagg ctcaagaaag gctcggggtc gggggaggaa
     6121 ttgttgagta aaaactacca cttggaaaat gaagtcgcca ggctcaaaaa agggagtggg
     6181 agcggcgaag agttattgag caaaaattac cacttggaga acgaagtggc aaggctcaag
     6241 aaagggagcg gcagcgggga ggagctctta tcgaagaact accacttaga gaatgaagtc
     6301 gcccgcttga agaaaggctc ggggagcggg gaagagctct tgagcaagaa ctaccacttg
     6361 gaaaatgagg tggcgcgctt gaagaaaggg agcgggagcg gggaagagtt actatctaag
     6421 aattatcatc tcgagaacga ggtggctcga ctaaagaagg gctccggcag tggggaggaa
     6481 ctcctgtcga agaactatca tcttgaaaat gaggttgcaa gacttaaaaa ggggtccgga
     6541 tcaggtgagg aactactcag taagaattac cacctggaaa acgaagttgc acgtttgaag
     6601 aaaggatcag gatcaggcga agaactgctc tcaaaagatt atcatttgga aaatgaggtt
     6661 gcacgtttaa aaaagggaag tggcagtggt gaggaacttc tgtcgaaaaa ttatcatctc
     6721 gagaatgaag tagcccgact taaaaagggt tctggctcgg gttaatgaga cttttttctt
     6781 ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt
     6841 tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaata gaattgaaga
     6901 gagattgtga aagtacatca gacagaatca cattttgata gcattcactt cactcagatg
     6961 caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt
     7021 agcgagagaa ttttttgaca aaaagaaaga agagtgatag agaagaaggg aatgcttgaa
     7081 aggattttgc atttatccat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7921 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9241 aaaagtgcca c
//
