LOCUS       sequence-402662-        2977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of short BRD4 isoform 
            tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4
FEATURES             Location/Qualifiers
     source          1..2977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..60
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             64..768
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
ORIGIN
        1 tgcagatatc cagcacagtg gcggccgatg ggaccggctg ccaagcgcgt gaaactcgat
       61 ggcgtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac
      121 atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca
      181 aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc
      241 tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg
      301 atgtcgcctt tccaggccgc aatggtagat ggctccggat accaagtcca tcgcacaatg
      361 cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac
      421 atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc
      481 aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa cgacaaaacc
      541 atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta ccggagcact
      601 gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa gaaccagccg
      661 atgtacgtgt tccgtaagac ggaactcaag cactccaaga ccgaactcaa cttcaaggag
      721 tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaaggg cggctcgtac
      781 gtgtacaagt ctgcggagag cggccctggg acgagattga gaaatctgcc agtaatgggg
      841 gatggactag aaacttccca aatgtctaca acacaggccc aggcccaacc ccagccagcc
      901 aacgcagcca gcaccaaccc cccgccccca gagacctcca accctaacaa gcccaagagg
      961 cagaccaacc aactgcaata cctgctcaga gtggtgctca agacactatg gaaacaccag
     1021 tttgcatggc ctttccagca gcctgtggat gccgtcaagc tgaacctccc tgattactat
     1081 aagatcatta aaacgcctat ggatatggga acaataaaga agcgcttgga aaacaactat
     1141 tactggaatg ctcaggaatg tatccaggac ttcaacacta tgtttacaaa ttgttacatc
     1201 tacaacaagc ctggagatga catagtctta atggcagaag ctctggaaaa gctcttcttg
     1261 caaaaaataa atgagctacc cacagaagaa accgagatca tgatagtcca ggcaaaagga
     1321 agaggacgtg ggaggaaaga aacagggaca gcaaaacctg gcgtttccac ggtaccaaac
     1381 acaactcaag catcgactcc tccgcagacc cagacccctc agccgaatcc tcctcctgtg
     1441 caggccacgc ctcacccctt ccctgccgtc accccggacc tcatcgtcca gacccctgtc
     1501 atgacagtgg tgcctcccca gccactgcag acgcccccgc cagtgccccc ccagccacaa
     1561 cccccacccg ctccagctcc ccagcccgta cagagccacc cacccatcat cgcggccacc
     1621 ccacagcctg tgaagacaaa gaagggagtg aagaggaaag cagacaccac cacccccacc
     1681 accattgacc ccattcacga gccaccctcg ctgcccccgg agcccaagac caccaagctg
     1741 ggccagcggc gggagagcag ccggcctgtg aaacctccaa agaaggacgt gcccgactct
     1801 cagcagcacc cagcaccaga gaagagcagc aaggtctcgg agcagctcaa gtgctgcagc
     1861 ggcatcctca aggagatgtt tgccaagaag cacgccgcct acgcctggcc cttctacaag
     1921 cctgtggacg tggaggcact gggcctacac gactactgtg acatcatcaa gcaccccatg
     1981 gacatgagca caatcaagtc taaactggag gcccgtgagt accgtgatgc tcaggagttt
     2041 ggtgctgacg tccgattgat gttctccaac tgctataagt acaaccctcc tgaccatgag
     2101 gtggtggcca tggcccgcaa gctccaggat gtgttcgaaa tgcgctttgc caagatgccg
     2161 gacgagcctg aggagccagt ggtggccgtg tcctccccgg cagtgccccc tcccaccaag
     2221 gttgtggccc cgccctcatc cagcgacagc agcagcgata gctcctcgga cagtgacagt
     2281 tcgactgatg actctgagga ggagcgagcc cagcggctgg ctgagctcca ggagcagctc
     2341 aaagccgtgc acgagcagct tgcagccctc tctcagcccc agcagaacaa accaaagaaa
     2401 aaggagaaag acaagaagga aaagaaaaaa gaaaagcaca aaaggaaaga ggaagtggaa
     2461 gagaataaaa aaagcaaagc caaggaacct cctcctaaaa agacgaagaa aaataatagc
     2521 agcaacagca atgtgagcaa gaaggagcca gcgcccatga agagcaagcc ccctcccacg
     2581 tatgagtcgg aggaagagga caagtgcaag cctatgtcct atgaggagaa gcggcagctc
     2641 agcttggaca tcaacaagct ccccggcgag aagctgggcc gcgtggtgca catcatccag
     2701 tcacgggagc cctccctgaa gaattccaac cccgacgaga ttgaaatcga ctttgagacc
     2761 ctgaagccgt ccacactgcg tgagctggag cgctatgtca cctcctgttt gcggaagaaa
     2821 aggaaacctc aagctgagaa agttgatgtg attgccggct cctccaagat gaagggcttc
     2881 tcgtcctcag agtcggagag ctccagtgag tccagctcct ctgacagcga agactccgaa
     2941 acaggctcga cctgatcata gtgaggctcg agtctag
//
