LOCUS       sequence-402663-        4200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of full-length NUT 
            tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-NUT
FEATURES             Location/Qualifiers
     source          1..4200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..56
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             60..764
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
ORIGIN
        1 gatatccagc acagtggcgg ccgatgggac cggctgccaa gcgcgtgaaa ctcgatggcg
       61 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
      121 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
      181 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
      241 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
      301 cgcctttcca ggccgcaatg gtagatggct ccggatacca agtccatcgc acaatgcagt
      361 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
      421 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
      481 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
      541 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
      601 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
      661 acgtgttccg taagacggaa ctcaagcact ccaagaccga actcaacttc aaggagtggc
      721 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggc tcgtacgagg
      781 ggtcgagcgc atctgcattg ccgggaccgg atatgagcat gaaacctagt gccgccctgt
      841 ctccatcccc tgcacttccc tttctcccac caacttctga cccaccagac cacccaccca
      901 gggagccacc tccacagccc atcatgcctt cagtattctc tccagacaac cctctgatgc
      961 tctctgcttt ccccagctca ctgttggtga caggggacgg gggcccttgc ctcagtgggg
     1021 ctggggctgg caaggtcatt gtcaaagtca agacagaagg ggggtcagct gagccctctc
     1081 aaactcagaa ctttatcctt actcagactg ccctcaattc gactgccccg ggcactccct
     1141 gtggaggcct tgagggtcct gcacctccat ttgtgacagc atctaatgtg aagaccattc
     1201 tgccctctaa ggctgttggt gtcagccagg agggtcctcc aggccttccg cctcagcctc
     1261 caccaccagt tgctcaactg gtccccattg tgcccctgga aaaagcttgg ccagggccac
     1321 atgggacaac cggggaagga ggtcctgtgg ccactctatc caagccttcc ctaggtgacc
     1381 gctccaaaat ttccaaggac gtttatgaga acttccgtca gtggcagcgt tacaaagcct
     1441 tggcccggag gcacctatcc cagagtcctg acacagaagc tctttcctgt tttcttatcc
     1501 cagtgcttcg ttccctggcc cggctgaagc ccactatgac cctggaggag ggactgccat
     1561 tggctgtgca ggagtgggag cacaccagca actttgaccg gatgatcttt tatgagatgg
     1621 cagaaaggtt catggagttt gaggctgagg agatgcagat tcagaacaca cagctgatga
     1681 atgggtctca gggcctgtct cctgcaaccc ctttgaaact tgatcctcta gggcccctgg
     1741 cctctgaggt ttgccagcag ccagtgtaca ttccgaagaa ggcagcctcc aagacacggg
     1801 ccccccgccg gcgtcagcgt aaagcccaga gacctcctgc tcctgaggca cccaaggaga
     1861 tcccaccaga agctgtgaag gagtatgttg acatcatgga atggctggtg gggactcact
     1921 tggccactgg ggagtcagat ggaaaacaag aggaagaagg gcagcagcag gaggaggaag
     1981 ggatgtatcc agatccaggt ctcctgagct acatcaatga gctgtgttct cagaaggtct
     2041 ttgtctccaa ggtggaggct gtcattcacc ctcaatttct ggcagatctg ctgtccccag
     2101 aaaaacagag agatcccttg gccttaattg aggagctaga gcaagaagaa ggactcactc
     2161 ttgcccagct ggtccagaag cgactcatgg ccttggaaga ggaggaagat gcagaggcgc
     2221 ctccaagttt cagtggcgct cagttggact caagtccttc tggttctgtt gaggatgaag
     2281 atggggatgg gcggcttcgg ccctcacctg ggcttcaggg ggctgggggc gccgcttgcc
     2341 ttggaaaggt ttcttcttca ggaaaacggg caagagaagt gcatggtggg caggagcaag
     2401 ccctagatag ccccagaggg atgcacaggg atgggaacac tctgccatcc cccagcagct
     2461 gggacctgca gccagaactt gcagctccac agggaactcc gggacccttg ggtgtggaga
     2521 ggagagggtc tgggaaggtt ataaaccagg tatctctaca tcaggatggc catctaggag
     2581 gcgctgggcc tcctgggcac tgcctggtgg ctgataggac ttcagaggct ctgccccttt
     2641 gttggcaggg aggcttccag cctgagagca ctcccagttt ggatgctgga cttgcagagc
     2701 tggctcctct gcaaggacaa gggttagaaa agcaagtcct gggattgcag aaaggacaac
     2761 aaacaggggg tcgtggagtg cttcctcaag ggaaggagcc tttagcagtg ccctgggaag
     2821 gctcttcagg agccatgtgg ggagatgaca gaggtacccc catggctcag agttatgatc
     2881 agaatccttc ccctagagca gctggggaga gggacgatgt ctgtctcagc ccaggagttt
     2941 ggctgagcag tgagatggat gctgtaggct tggagctgcc tgtacaaata gaggaggtca
     3001 tagagagctt ccaagttgag aagtgtgtaa ctgagtatca ggaaggctgc cagggactgg
     3061 gctccagggg caacatttcc ctgggtcctg gagaaaccct agtacctggg gatacggaga
     3121 gcagtgtgat tccctgtgga ggcacagttg cggcagctgc cctagaaaag agaaactatt
     3181 gcagcttgcc aggacctttg agggccaaca gcccaccctt gaggtccaaa gaaaatcaag
     3241 aacagagctg tgaaaccgta gggcatccca gtgatctgtg ggcagaaggt tgcttcccat
     3301 tgctagaaag tggtgattcc acactggggt cttccaaaga aacccttcca cccacatgcc
     3361 aaggcaatct ccttatcatg gggactgagg atgcctcctc cttgcctgaa gccagccaag
     3421 aggcagggag cagaggcaat tccttttctc ctctgttgga aaccatagaa cctgtcaaca
     3481 tactagatgt taaagatgac tgtggcctcc aactaagggt cagcgaggac acctgcccac
     3541 tgaatgttca ttcttatgac ccccaaggag aaggcagggt ggatcctgat ctgtccaagc
     3601 ctaaaaacct tgctccttta caagagagtc aggagtctta cacaactggg actcccaaag
     3661 caacatcttc tcaccagggc cttggaagca ctttgcctag aaggggaacc aggaatgcca
     3721 tagttccgag agaaacttct gttagtaaaa cacacaggtc agcagacagg gccaaaggaa
     3781 aggagaaaaa gaaaaaggaa gcagaggaag aggatgagga actctccaac tttgcttacc
     3841 tcttggcctc taaacttagc ctctcaccaa gggagcatcc cctcagtcct caccatgcct
     3901 caggaggtca gggcagccag agagcatccc acctgctccc tgctggagca aaaggcccca
     3961 gcaaacttcc atatcctgtt gccaagtctg ggaagcgagc tctagctgga ggtccagccc
     4021 ctactgaaaa gacaccccac tcaggagctc aacttggggt ccccagggag aaacccctag
     4081 ctctgggagt agttcgaccc tcacagcctc gtaaaaggcg gtgtgacagt tttgtcacgg
     4141 gcagaaggaa gaaacgacgt cgtagccagg gctcgacctg atcatagtga ggctcgagtc
//
