LOCUS       sequence-402664-        6409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of full-length BRD4-NUT
            fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT
FEATURES             Location/Qualifiers
     source          1..6409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..110
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             114..818
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
ORIGIN
        1 cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
       61 cagcacagtg gcggccgatg ggaccggctg ccaagcgcgt gaaactcgat ggcgtgagca
      121 agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac atctttggct
      181 ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca aatgatggtt
      241 atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc tggattctgg
      301 tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg atgtcgcctt
      361 tccaggccgc aatggtagat ggctccggat accaagtcca tcgcacaatg cagtttgaag
      421 atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac atcaaaggag
      481 aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc aactcgctga
      541 ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa cgacaaaacc atcatcagta
      601 cctttaagtg gagttacacc actggaaatg gcaagcgcta ccggagcact gcgcggacca
      661 cctacacctt tgccaagcca atggcggcta actatctgaa gaaccagccg atgtacgtgt
      721 tccgtaagac ggaactcaag cactccaaga ccgaactcaa cttcaaggag tggcaaaagg
      781 cctttaccga tgtgatgggc atggacgagc tgtacaaggg cggctcgtac gtgtacaagt
      841 ctgcggagag cggccctggg acgagattga gaaatctgcc agtaatgggg gatggactag
      901 aaacttccca aatgtctaca acacaggccc aggcccaacc ccagccagcc aacgcagcca
      961 gcaccaaccc cccgccccca gagacctcca accctaacaa gcccaagagg cagaccaacc
     1021 aactgcaata cctgctcaga gtggtgctca agacactatg gaaacaccag tttgcatggc
     1081 ctttccagca gcctgtggat gccgtcaagc tgaacctccc tgattactat aagatcatta
     1141 aaacgcctat ggatatggga acaataaaga agcgcttgga aaacaactat tactggaatg
     1201 ctcaggaatg tatccaggac ttcaacacta tgtttacaaa ttgttacatc tacaacaagc
     1261 ctggagatga catagtctta atggcagaag ctctggaaaa gctcttcttg caaaaaataa
     1321 atgagctacc cacagaagaa accgagatca tgatagtcca ggcaaaagga agaggacgtg
     1381 ggaggaaaga aacagggaca gcaaaacctg gcgtttccac ggtaccaaac acaactcaag
     1441 catcgactcc tccgcagacc cagacccctc agccgaatcc tcctcctgtg caggccacgc
     1501 ctcacccctt ccctgccgtc accccggacc tcatcgtcca gacccctgtc atgacagtgg
     1561 tgcctcccca gccactgcag acgcccccgc cagtgccccc ccagccacaa cccccacccg
     1621 ctccagctcc ccagcccgta cagagccacc cacccatcat cgcggccacc ccacagcctg
     1681 tgaagacaaa gaagggagtg aagaggaaag cagacaccac cacccccacc accattgacc
     1741 ccattcacga gccaccctcg ctgcccccgg agcccaagac caccaagctg ggccagcggc
     1801 gggagagcag ccggcctgtg aaacctccaa agaaggacgt gcccgactct cagcagcacc
     1861 cagcaccaga gaagagcagc aaggtctcgg agcagctcaa gtgctgcagc ggcatcctca
     1921 aggagatgtt tgccaagaag cacgccgcct acgcctggcc cttctacaag cctgtggacg
     1981 tggaggcact gggcctacac gactactgtg acatcatcaa gcaccccatg gacatgagca
     2041 caatcaagtc taaactggag gcccgtgagt accgtgatgc tcaggagttt ggtgctgacg
     2101 tccgattgat gttctccaac tgctataagt acaaccctcc tgaccatgag gtggtggcca
     2161 tggcccgcaa gctccaggat gtgttcgaaa tgcgctttgc caagatgccg gacgagcctg
     2221 aggagccagt ggtggccgtg tcctccccgg cagtgccccc tcccaccaag gttgtggccc
     2281 cgccctcatc cagcgacagc agcagcgata gctcctcgga cagtgacagt tcgactgatg
     2341 actctgagga ggagcgagcc cagcggctgg ctgagctcca ggagcagctc aaagccgtgc
     2401 acgagcagct tgcagccctc tctcagcccc agcagaacaa accaaagaaa aaggagaaag
     2461 acaagaagga aaagaaaaaa gaaaagcaca aaaggaaaga ggaagtggaa gagaataaaa
     2521 aaagcaaagc caaggaacct cctcctaaaa agacgaagaa aaataatagc agcaacagca
     2581 atgtgagcaa gaaggagcca gcgcccatga agagcaagcc ccctcccacg tatgagtcgg
     2641 aggaagagga caagtgcaag cctatgtcct atgaggagaa gcggcagctc agcttggaca
     2701 tcaacaagct ccccggcgag aagctgggcc gcgtggtgca catcatccag tcacgggagc
     2761 cctccctgaa gaattccaac cccgacgaga ttgaaatcga ctttgagacc ctgaagccgt
     2821 ccacactgcg tgagctggag cgctatgtca cctcctgttt gcggaagaaa aggaaacctc
     2881 aagctgagaa agttgatgtg attgccggct cctccaagat gaagggcttc tcgtcctcag
     2941 agtcggagag ctccagtgag tccagctcct ctgacagcga agactccgaa acagcatctg
     3001 cattgccggg accggatatg agcatgaaac ctagtgccgc cctgtctcca tcccctgcac
     3061 ttccctttct cccaccaact tctgacccac cagaccaccc acccagggag ccacctccac
     3121 agcccatcat gccttcagta ttctctccag acaaccctct gatgctctct gctttcccca
     3181 gctcactgtt ggtgacaggg gacgggggcc cttgcctcag tggggctggg gctggcaagg
     3241 tcattgtcaa agtcaagaca gaaggggggt cagctgagcc ctctcaaact cagaacttta
     3301 tccttactca gactgccctc aattcgactg ccccgggcac tccctgtgga ggccttgagg
     3361 gtcctgcacc tccatttgtg acagcatcta atgtgaagac cattctgccc tctaaggctg
     3421 ttggtgtcag ccaggagggt cctccaggcc ttccgcctca gcctccacca ccagttgctc
     3481 aactggtccc cattgtgccc ctggaaaaag cttggccagg gccacatggg acaaccgggg
     3541 aaggaggtcc tgtggccact ctatccaagc cttccctagg tgaccgctcc aaaatttcca
     3601 aggacgttta tgagaacttc cgtcagtggc agcgttacaa agccttggcc cggaggcacc
     3661 tatcccagag tcctgacaca gaagctcttt cctgttttct tatcccagtg cttcgttccc
     3721 tggcccggct gaagcccact atgaccctgg aggagggact gccattggct gtgcaggagt
     3781 gggagcacac cagcaacttt gaccggatga tcttttatga gatggcagaa aggttcatgg
     3841 agtttgaggc tgaggagatg cagattcaga acacacagct gatgaatggg tctcagggcc
     3901 tgtctcctgc aacccctttg aaacttgatc ctctagggcc cctggcctct gaggtttgcc
     3961 agcagccagt gtacattccg aagaaggcag cctccaagac acgggccccc cgccggcgtc
     4021 agcgtaaagc ccagagacct cctgctcctg aggcacccaa ggagatccca ccagaagctg
     4081 tgaaggagta tgttgacatc atggaatggc tggtggggac tcacttggcc actggggagt
     4141 cagatggaaa acaagaggaa gaagggcagc agcaggagga ggaagggatg tatccagatc
     4201 caggtctcct gagctacatc aatgagctgt gttctcagaa ggtctttgtc tccaaggtgg
     4261 aggctgtcat tcaccctcaa tttctggcag atctgctgtc cccagaaaaa cagagagatc
     4321 ccttggcctt aattgaggag ctagagcaag aagaaggact cactcttgcc cagctggtcc
     4381 agaagcgact catggccttg gaagaggagg aagatgcaga ggcgcctcca agtttcagtg
     4441 gcgctcagtt ggactcaagt ccttctggtt ctgttgagga tgaagatggg gatgggcggc
     4501 ttcggccctc acctgggctt cagggggctg ggggcgccgc ttgccttgga aaggtttctt
     4561 cttcaggaaa acgggcaaga gaagtgcatg gtgggcagga gcaagcccta gatagcccca
     4621 gagggatgca cagggatggg aacactctgc catcccccag cagctgggac ctgcagccag
     4681 aacttgcagc tccacaggga actccgggac ccttgggtgt ggagaggaga gggtctggga
     4741 aggttataaa ccaggtatct ctacatcagg atggccatct aggaggcgct gggcctcctg
     4801 ggcactgcct ggtggctgat aggacttcag aggctctgcc cctttgttgg cagggaggct
     4861 tccagcctga gagcactccc agtttggatg ctggacttgc agagctggct cctctgcaag
     4921 gacaagggtt agaaaagcaa gtcctgggat tgcagaaagg acaacaaaca gggggtcgtg
     4981 gagtgcttcc tcaagggaag gagcctttag cagtgccctg ggaaggctct tcaggagcca
     5041 tgtggggaga tgacagaggt acccccatgg ctcagagtta tgatcagaat ccttccccta
     5101 gagcagctgg ggagagggac gatgtctgtc tcagcccagg agtttggctg agcagtgaga
     5161 tggatgctgt aggcttggag ctgcctgtac aaatagagga ggtcatagag agcttccaag
     5221 ttgagaagtg tgtaactgag tatcaggaag gctgccaggg actgggctcc aggggcaaca
     5281 tttccctggg tcctggagaa accctagtac ctggggatac ggagagcagt gtgattccct
     5341 gtggaggcac agttgcggca gctgccctag aaaagagaaa ctattgcagc ttgccaggac
     5401 ctttgagggc caacagccca cccttgaggt ccaaagaaaa tcaagaacag agctgtgaaa
     5461 ccgtagggca tcccagtgat ctgtgggcag aaggttgctt cccattgcta gaaagtggtg
     5521 attccacact ggggtcttcc aaagaaaccc ttccacccac atgccaaggc aatctcctta
     5581 tcatggggac tgaggatgcc tcctccttgc ctgaagccag ccaagaggca gggagcagag
     5641 gcaattcctt ttctcctctg ttggaaacca tagaacctgt caacatacta gatgttaaag
     5701 atgactgtgg cctccaacta agggtcagcg aggacacctg cccactgaat gttcattctt
     5761 atgaccccca aggagaaggc agggtggatc ctgatctgtc caagcctaaa aaccttgctc
     5821 ctttacaaga gagtcaggag tcttacacaa ctgggactcc caaagcaaca tcttctcacc
     5881 agggccttgg aagcactttg cctagaaggg gaaccaggaa tgccatagtt ccgagagaaa
     5941 cttctgttag taaaacacac aggtcagcag acagggccaa aggaaaggag aaaaagaaaa
     6001 aggaagcaga ggaagaggat gaggaactct ccaactttgc ttacctcttg gcctctaaac
     6061 ttagcctctc accaagggag catcccctca gtcctcacca tgcctcagga ggtcagggca
     6121 gccagagagc atcccacctg ctccctgctg gagcaaaagg ccccagcaaa cttccatatc
     6181 ctgttgccaa gtctgggaag cgagctctag ctggaggtcc agcccctact gaaaagacac
     6241 cccactcagg agctcaactt ggggtcccca gggagaaacc cctagctctg ggagtagttc
     6301 gaccctcaca gcctcgtaaa aggcggtgtg acagttttgt cacgggcaga aggaagaaac
     6361 gacgtcgtag ccagggctcg acctgatcat agtgaggctc gagtctaga
//
