LOCUS       sequence-402665-        3434 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant
            fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)
FEATURES             Location/Qualifiers
     source          1..3434
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..64
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             68..772
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
ORIGIN
        1 attctgcaga tatccagcac agtggcggcc gatgggaccg gctgccaagc gcgtgaaact
       61 cgatggcgtg agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt
      121 acacatcttt ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa
      181 tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc
      241 cccctggatt ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga
      301 cgggatgtcg cctttccagg ccgcaatggt agatggctcc ggataccaag tccatcgcac
      361 aatgcagttt gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag
      421 ccacatcaaa ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat
      481 gaccaactcg ctgaccgctg cggactggtg caggtcgaag aagacttacc ccaacgacaa
      541 aaccatcatc agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag
      601 cactgcgcgg accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca
      661 gccgatgtac gtgttccgta agacggaact caagcactcc aagaccgaac tcaacttcaa
      721 ggagtggcaa aaggccttta ccgatgtgat gggcatggac gagctgtaca agggcggctc
      781 gtacgtgtac aagtctgcgg agagcggccc tgggacgaga ttgagaaatc tgccagtaat
      841 gggggatgga ctagaaactt cccaaatgtc tacaacacag gcccaggccc aaccccagcc
      901 agccaacgca gccagcacca accccccgcc cccagagacc tccaacccta acaagcccaa
      961 gaggcagacc aaccaactgc aatacctgct cagagtggtg ctcaagacac tatggaaaca
     1021 ccagtttgca tggcctttcc agcagcctgt ggatgccgtc aagctgaacc tccctgatta
     1081 ctataagatc attaaaacgc ctatggatat gggaacaata aagaagcgct tggaaaacaa
     1141 ctattactgg aatgctcagg aatgtatcca ggacttcaac actatgttta caaattgtta
     1201 catctacaac aagcctggag atgacatagt cttaatggca gaagctctgg aaaagctctt
     1261 cttgcaaaaa ataaatgagc tacccacaga agaaaccgag atcatgatag tccaggcaaa
     1321 aggaagagga cgtgggagga aagaaacagg gacagcaaaa cctggcgttt ccacggtacc
     1381 aaacacaact caagcatcga ctcctccgca gacccagacc cctcagccga atcctcctcc
     1441 tgtgcaggcc acgcctcacc ccttccctgc cgtcaccccg gacctcatcg tccagacccc
     1501 tgtcatgaca gtggtgcctc cccagccact gcagacgccc ccgccagtgc ccccccagcc
     1561 acaaccccca cccgctccag ctccccagcc cgtacagagc cacccaccca tcatcgcggc
     1621 caccccacag cctgtgaaga caaagaaggg agtgaagagg aaagcagaca ccaccacccc
     1681 caccaccatt gaccccattc acgagccacc ctcgctgccc ccggagccca agaccaccaa
     1741 gctgggccag cggcgggaga gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga
     1801 ctctcagcag cacccagcac cagagaagag cagcaaggtc tcggagcagc tcaagtgctg
     1861 cagcggcatc ctcaaggaga tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta
     1921 caagcctgtg gacgtggagg cactgggcct acacgactac tgtgacatca tcaagcaccc
     1981 catggacatg agcacaatca agtctaaact ggaggcccgt gagtaccgtg atgctcagga
     2041 gtttggtgct gacgtccgat tgatgttctc caactgctat aagtacaacc ctcctgacca
     2101 tgaggtggtg gccatggccc gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat
     2161 gccggacgag cctgaggagc cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac
     2221 caaggttgtg gccccgccct catccagcga cagcagcagc gatagctcct cggacagtga
     2281 cagttcgact gatgactctg aggaggagcg agcccagcgg ctggctgagc tccaggagca
     2341 gctcaaagcc gtgcacgagc agcttgcagc cctctctcag ccccagcaga acaaaccaaa
     2401 gaaaaaggag aaagacaaga aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt
     2461 ggaagagaat aaaaaaagca aagccaagga acctcctcct aaaaagacga agaaaaataa
     2521 tagcagcaac agcaatgtga gcaagaagga gccagcgccc atgaagagca agccccctcc
     2581 cacgtatgag tcggaggaag aggacaagtg caagcctatg tcctatgagg agaagcggca
     2641 gctcagcttg gacatcaaca agctccccgg cgagaagctg ggccgcgtgg tgcacatcat
     2701 ccagtcacgg gagccctccc tgaagaattc caaccccgac gagattgaaa tcgactttga
     2761 gaccctgaag ccgtccacac tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa
     2821 gaaaaggaaa cctcaagctg agaaagttga tgtgattgcc ggctcctcca agatgaaggg
     2881 cttctcgtcc tcagagtcgg agagctccag tgagtccagc tcctctgaca gcgaagactc
     2941 cgaaacacct gctcctgagg cacccaagga aatcccacca gaagctgtga aggagtatgt
     3001 tgacatcatg gaatggctgg tggggactca cttggccact ggggagtcag atggaaaaca
     3061 agaggaagaa gggcagcagc aggaggagga agggatgtat ccagatccag gtctcctgag
     3121 ctacatcaat gagctgtgtt ctcagaaggt ctttgtctcc aaggtggagg ctgtcattca
     3181 ccctcaattt ctggcagatc tgctgtcccc agaaaaacag agagatccct tggccttaat
     3241 tgaggagcta gagcaagaag aaggactcac tcttgcccag ctggtccaga agcgactcat
     3301 ggccttggaa gaggaggaag atgcagaggc gcctccaagt ttcagtggcg ctcagttgga
     3361 ctcaagtcct tctggttctg ttgaggatga agatggggat ggctcgacct gatcatagtg
     3421 aggctcgagt ctag
//
