LOCUS       sequence-402666-        5916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) 
            mutant fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)
FEATURES             Location/Qualifiers
     source          1..5916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..70
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             74..778
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
ORIGIN
        1 ggtggaattc tgcagatatc cagcacagtg gcggccgatg ggaccggctg ccaagcgcgt
       61 gaaactcgat ggcgtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca
      121 tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac
      181 cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca
      241 gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta
      301 ccctgacggg atgtcgcctt tccaggccgc aatggtagat ggctccggat accaagtcca
      361 tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga
      421 gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc
      481 tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa
      541 cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta
      601 ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa
      661 gaaccagccg atgtacgtgt tccgtaagac ggaactcaag cactccaaga ccgaactcaa
      721 cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaaggg
      781 cggctcgtac gtgtacaagt ctgcggagag cggccctggg acgagattga gaaatctgcc
      841 agtaatgggg gatggactag aaacttccca aatgtctaca acacaggccc aggcccaacc
      901 ccagccagcc aacgcagcca gcaccaaccc cccgccccca gagacctcca accctaacaa
      961 gcccaagagg cagaccaacc aactgcaata cctgctcaga gtggtgctca agacactatg
     1021 gaaacaccag tttgcatggc ctttccagca gcctgtggat gccgtcaagc tgaacctccc
     1081 tgattactat aagatcatta aaacgcctat ggatatggga acaataaaga agcgcttgga
     1141 aaacaactat tactggaatg ctcaggaatg tatccaggac ttcaacacta tgtttacaaa
     1201 ttgttacatc tacaacaagc ctggagatga catagtctta atggcagaag ctctggaaaa
     1261 gctcttcttg caaaaaataa atgagctacc cacagaagaa accgagatca tgatagtcca
     1321 ggcaaaagga agaggacgtg ggaggaaaga aacagggaca gcaaaacctg gcgtttccac
     1381 ggtaccaaac acaactcaag catcgactcc tccgcagacc cagacccctc agccgaatcc
     1441 tcctcctgtg caggccacgc ctcacccctt ccctgccgtc accccggacc tcatcgtcca
     1501 gacccctgtc atgacagtgg tgcctcccca gccactgcag acgcccccgc cagtgccccc
     1561 ccagccacaa cccccacccg ctccagctcc ccagcccgta cagagccacc cacccatcat
     1621 cgcggccacc ccacagcctg tgaagacaaa gaagggagtg aagaggaaag cagacaccac
     1681 cacccccacc accattgacc ccattcacga gccaccctcg ctgcccccgg agcccaagac
     1741 caccaagctg ggccagcggc gggagagcag ccggcctgtg aaacctccaa agaaggacgt
     1801 gcccgactct cagcagcacc cagcaccaga gaagagcagc aaggtctcgg agcagctcaa
     1861 gtgctgcagc ggcatcctca aggagatgtt tgccaagaag cacgccgcct acgcctggcc
     1921 cttctacaag cctgtggacg tggaggcact gggcctacac gactactgtg acatcatcaa
     1981 gcaccccatg gacatgagca caatcaagtc taaactggag gcccgtgagt accgtgatgc
     2041 tcaggagttt ggtgctgacg tccgattgat gttctccaac tgctataagt acaaccctcc
     2101 tgaccatgag gtggtggcca tggcccgcaa gctccaggat gtgttcgaaa tgcgctttgc
     2161 caagatgccg gacgagcctg aggagccagt ggtggccgtg tcctccccgg cagtgccccc
     2221 tcccaccaag gttgtggccc cgccctcatc cagcgacagc agcagcgata gctcctcgga
     2281 cagtgacagt tcgactgatg actctgagga ggagcgagcc cagcggctgg ctgagctcca
     2341 ggagcagctc aaagccgtgc acgagcagct tgcagccctc tctcagcccc agcagaacaa
     2401 accaaagaaa aaggagaaag acaagaagga aaagaaaaaa gaaaagcaca aaaggaaaga
     2461 ggaagtggaa gagaataaaa aaagcaaagc caaggaacct cctcctaaaa agacgaagaa
     2521 aaataatagc agcaacagca atgtgagcaa gaaggagcca gcgcccatga agagcaagcc
     2581 ccctcccacg tatgagtcgg aggaagagga caagtgcaag cctatgtcct atgaggagaa
     2641 gcggcagctc agcttggaca tcaacaagct ccccggcgag aagctgggcc gcgtggtgca
     2701 catcatccag tcacgggagc cctccctgaa gaattccaac cccgacgaga ttgaaatcga
     2761 ctttgagacc ctgaagccgt ccacactgcg tgagctggag cgctatgtca cctcctgttt
     2821 gcggaagaaa aggaaacctc aagctgagaa agttgatgtg attgccggct cctccaagat
     2881 gaagggcttc tcgtcctcag agtcggagag ctccagtgag tccagctcct ctgacagcga
     2941 agactccgaa acagcatctg cattgccggg accggatatg agcatgaaac ctagtgccgc
     3001 cctgtctcca tcccctgcac ttccctttct cccaccaact tctgacccac cagaccaccc
     3061 acccagggag ccacctccac agcccatcat gccttcagta ttctctccag acaaccctct
     3121 gatgctctct gctttcccca gctcactgtt ggtgacaggg gacgggggcc cttgcctcag
     3181 tggggctggg gctggcaagg tcattgtcaa agtcaagaca gaaggggggt cagctgagcc
     3241 ctctcaaact cagaacttta tccttactca gactgccctc aattcgactg ccccgggcac
     3301 tccctgtgga ggccttgagg gtcctgcacc tccatttgtg acagcatcta atgtgaagac
     3361 cattctgccc tctaaggctg ttggtgtcag ccaggagggt cctccaggcc ttccgcctca
     3421 gcctccacca ccagttgctc aactggtccc cattgtgccc ctggaaaaag cttggccagg
     3481 gccacatggg acaaccgggg aaggaggtcc tgtggccact ctatccaagc cttccctagg
     3541 tgaccgctcc aaaatttcca aggacgttta tgagaacttc cgtcagtggc agcgttacaa
     3601 agccttggcc cggaggcacc tatcccagag tcctgacaca gaagctcttt cctgttttct
     3661 tatcccagtg cttcgttccc tggcccggct gaagcccact atgaccctgg aggagggact
     3721 gccattggct gtgcaggagt gggagcacac cagcaacttt gaccggatga tcttttatga
     3781 gatggcagaa aggttcatgg agtttgaggc tgaggagatg cagattcaga acacacagct
     3841 gatgaatggg tctcagggcc tgtctcctgc aacccctttg aaacttgatc ctctagggcc
     3901 cctggcctct gaggtttgcc agcagccagt gtacattccg aagaaggcag cctccaagac
     3961 acgggccccc cgccggcgtc agcgtaaagc ccagagacct gggcggcttc ggccctcacc
     4021 tgggcttcag ggggctgggg gcgccgcttg ccttggaaag gtttcttctt caggaaaacg
     4081 ggcaagagaa gtgcatggtg ggcaggagca agccctagat agccccagag ggatgcacag
     4141 ggatgggaac actctgccat cccccagcag ctgggacctg cagccagaac ttgcagctcc
     4201 acagggaact ccgggaccct tgggtgtgga gaggagaggg tctgggaagg ttataaacca
     4261 ggtatctcta catcaggatg gccatctagg aggcgctggg cctcctgggc actgcctggt
     4321 ggctgatagg acttcagagg ctctgcccct ttgttggcag ggaggcttcc agcctgagag
     4381 cactcccagt ttggatgctg gacttgcaga gctggctcct ctgcaaggac aagggttaga
     4441 aaagcaagtc ctgggattgc agaaaggaca acaaacaggg ggtcgtggag tgcttcctca
     4501 agggaaggag cctttagcag tgccctggga aggctcttca ggagccatgt ggggagatga
     4561 cagaggtacc cccatggctc agagttatga tcagaatcct tcccctagag cagctgggga
     4621 gagggacgat gtctgtctca gcccaggagt ttggctgagc agtgagatgg atgctgtagg
     4681 cttggagctg cctgtacaaa tagaggaggt catagagagc ttccaagttg agaagtgtgt
     4741 aactgagtat caggaaggct gccagggact gggctccagg ggcaacattt ccctgggtcc
     4801 tggagaaacc ctagtacctg gggatacgga gagcagtgtg attccctgtg gaggcacagt
     4861 tgcggcagct gccctagaaa agagaaacta ttgcagcttg ccaggacctt tgagggccaa
     4921 cagcccaccc ttgaggtcca aagaaaatca agaacagagc tgtgaaaccg tagggcatcc
     4981 cagtgatctg tgggcagaag gttgcttccc attgctagaa agtggtgatt ccacactggg
     5041 gtcttccaaa gaaacccttc cacccacatg ccaaggcaat ctccttatca tggggactga
     5101 ggatgcctcc tccttgcctg aagccagcca agaggcaggg agcagaggca attccttttc
     5161 tcctctgttg gaaaccatag aacctgtcaa catactagat gttaaagatg actgtggcct
     5221 ccaactaagg gtcagcgagg acacctgccc actgaatgtt cattcttatg acccccaagg
     5281 agaaggcagg gtggatcctg atctgtccaa gcctaaaaac cttgctcctt tacaagagag
     5341 tcaggagtct tacacaactg ggactcccaa agcaacatct tctcaccagg gccttggaag
     5401 cactttgcct agaaggggaa ccaggaatgc catagttccg agagaaactt ctgttagtaa
     5461 aacacacagg tcagcagaca gggccaaagg aaaggagaaa aagaaaaagg aagcagagga
     5521 agaggatgag gaactctcca actttgctta cctcttggcc tctaaactta gcctctcacc
     5581 aagggagcat cccctcagtc ctcaccatgc ctcaggaggt cagggcagcc agagagcatc
     5641 ccacctgctc cctgctggag caaaaggccc cagcaaactt ccatatcctg ttgccaagtc
     5701 tgggaagcga gctctagctg gaggtccagc ccctactgaa aagacacccc actcaggagc
     5761 tcaacttggg gtccccaggg agaaacccct agctctggga gtagttcgac cctcacagcc
     5821 tcgtaaaagg cggtgtgaca gttttgtcac gggcagaagg aagaaacgac gtcgtagcca
     5881 gggctcgacc tgatcatagt gaggctcgag tctaga
//
