LOCUS       sequence-402668-       10184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             46..750
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             6067..7917
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300, inactivated by the 
                     D1399Y point mutation (Hilton et al., 2015)"
                     /label=p300 core (D1399Y)
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLYSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      7681..7690
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttaagctta tgggaccggc tgccaagcgc gtgaaactcg atggcgtgag caagggcgag
       61 gaggataaca tggcctctct cccagcgaca catgagttac acatctttgg ctccatcaac
      121 ggtgtggact ttgacatggt gggtcagggc accggcaatc caaatgatgg ttatgaggag
      181 ttaaacctga agtccaccaa gggtgacctc cagttctccc cctggattct ggtccctcat
      241 atcgggtatg gcttccatca gtacctgccc taccctgacg ggatgtcgcc tttccaggcc
      301 gcaatggtag atggctccgg ataccaagtc catcgcacaa tgcagtttga agatggtgcc
      361 tcccttactg ttaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag
      421 gtgaagggga ctggtttccc tgctgacggt cctgtgatga ccaactcgct gaccgctgcg
      481 gactggtgca ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag tacctttaag
      541 tggagttaca ccactggaaa tggcaagcgc taccggagca ctgcgcggac cacctacacc
      601 tttgccaagc caatggcggc taactatctg aagaaccagc cgatgtacgt gttccgtaag
      661 acggaactca agcactccaa gaccgaactc aacttcaagg agtggcaaaa ggcctttacc
      721 gatgtgatgg gcatggacga gctgtacaag ggcggctcgt acgtgtacaa gtctgcggag
      781 agcggccctg ggacgagatt gagaaatctg ccagtaatgg gggatggact agaaacttcc
      841 caaatgtcta caacacaggc ccaggcccaa ccccagccag ccaacgcagc cagcaccaac
      901 cccccgcccc cagagacctc caaccctaac aagcccaaga ggcagaccaa ccaactgcaa
      961 tacctgctca gagtggtgct caagacacta tggaaacacc agtttgcatg gcctttccag
     1021 cagcctgtgg atgccgtcaa gctgaacctc cctgattact ataagatcat taaaacgcct
     1081 atggatatgg gaacaataaa gaagcgcttg gaaaacaact attactggaa tgctcaggaa
     1141 tgtatccagg acttcaacac tatgtttaca aattgttaca tctacaacaa gcctggagat
     1201 gacatagtct taatggcaga agctctggaa aagctcttct tgcaaaaaat aaatgagcta
     1261 cccacagaag aaaccgagat catgatagtc caggcaaaag gaagaggacg tgggaggaaa
     1321 gaaacaggga cagcaaaacc tggcgtttcc acggtaccaa acacaactca agcatcgact
     1381 cctccgcaga cccagacccc tcagccgaat cctcctcctg tgcaggccac gcctcacccc
     1441 ttccctgccg tcaccccgga cctcatcgtc cagacccctg tcatgacagt ggtgcctccc
     1501 cagccactgc agacgccccc gccagtgccc ccccagccac aacccccacc cgctccagct
     1561 ccccagcccg tacagagcca cccacccatc atcgcggcca ccccacagcc tgtgaagaca
     1621 aagaagggag tgaagaggaa agcagacacc accaccccca ccaccattga ccccattcac
     1681 gagccaccct cgctgccccc ggagcccaag accaccaagc tgggccagcg gcgggagagc
     1741 agccggcctg tgaaacctcc aaagaaggac gtgcccgact ctcagcagca cccagcacca
     1801 gagaagagca gcaaggtctc ggagcagctc aagtgctgca gcggcatcct caaggagatg
     1861 tttgccaaga agcacgccgc ctacgcctgg cccttctaca agcctgtgga cgtggaggca
     1921 ctgggcctac acgactactg tgacatcatc aagcacccca tggacatgag cacaatcaag
     1981 tctaaactgg aggcccgtga gtaccgtgat gctcaggagt ttggtgctga cgtccgattg
     2041 atgttctcca actgctataa gtacaaccct cctgaccatg aggtggtggc catggcccgc
     2101 aagctccagg atgtgttcga aatgcgcttt gccaagatgc cggacgagcc tgaggagcca
     2161 gtggtggccg tgtcctcccc ggcagtgccc cctcccacca aggttgtggc cccgccctca
     2221 tccagcgaca gcagcagcga tagctcctcg gacagtgaca gttcgactga tgactctgag
     2281 gaggagcgag cccagcggct ggctgagctc caggagcagc tcaaagccgt gcacgagcag
     2341 cttgcagccc tctctcagcc ccagcagaac aaaccaaaga aaaaggagaa agacaagaag
     2401 gaaaagaaaa aagaaaagca caaaaggaaa gaggaagtgg aagagaataa aaaaagcaaa
     2461 gccaaggaac ctcctcctaa aaagacgaag aaaaataata gcagcaacag caatgtgagc
     2521 aagaaggagc cagcgcccat gaagagcaag ccccctccca cgtatgagtc ggaggaagag
     2581 gacaagtgca agcctatgtc ctatgaggag aagcggcagc tcagcttgga catcaacaag
     2641 ctccccggcg agaagctggg ccgcgtggtg cacatcatcc agtcacggga gccctccctg
     2701 aagaattcca accccgacga gattgaaatc gactttgaga ccctgaagcc gtccacactg
     2761 cgtgagctgg agcgctatgt cacctcctgt ttgcggaaga aaaggaaacc tcaagctgag
     2821 aaagttgatg tgattgccgg ctcctccaag atgaagggct tctcgtcctc agagtcggag
     2881 agctccagtg agtccagctc ctctgacagc gaagactccg aaacaatggc cgagaatgtg
     2941 gtggaaccgg ggccgccttc agccaagcgg cctaaactct catctccggc cctctcggcg
     3001 tccgccagcg atggcacaga ttttggctct ctatttgact tggagcacga cttaccagat
     3061 gaattaatca actctacaga attgggacta accaatggtg gtgatattaa tcagcttcag
     3121 acaagtcttg gcatggtaca agatgcagct tctaaacata aacagctgtc agaattgctg
     3181 cgatctggta gttcccctaa cctcaatatg ggagttggtg gcccaggtca agtcatggcc
     3241 agccaggccc aacagagcag tcctggatta ggtttgataa atagcatggt caaaagccca
     3301 atgacacagg caggcttgac ttctcccaac atggggatgg gcactagtgg accaaatcag
     3361 ggtcctacgc agtcaacagg tatgatgaac agtccagtaa atcagcctgc catgggaatg
     3421 aacacaggga tgaatgcggg catgaatcct ggaatgttgg ctgcaggcaa tggacaaggg
     3481 ataatgccta atcaagtcat gaacggttca attggagcag gccgagggcg acagaatatg
     3541 cagtacccaa acccaggcat gggaagtgct ggcaacttac tgactgagcc tcttcagcag
     3601 ggctctcccc agatgggagg acaaacagga ttgagaggcc cccagcctct taagatggga
     3661 atgatgaaca accccaatcc ttatggttca ccatatactc agaatcctgg acagcagatt
     3721 ggagccagtg gccttggtct ccagattcag acaaaaactg tactatcaaa taacttatct
     3781 ccatttgcta tggacaaaaa ggcagttcct ggtggaggaa tgcccaacat gggtcaacag
     3841 ccagccccgc aggtccagca gccaggtctg gtgactccag ttgcccaagg gatgggttct
     3901 ggagcacata cagctgatcc agagaagcgc aagctcatcc agcagcagct tgttctcctt
     3961 ttgcatgctc acaagtgcca gcgccgggaa caggccaatg gggaagtgag gcagtgcaac
     4021 cttccccact gtcgcacaat gaagaatgtc ctaaaccaca tgacacactg ccagtcaggc
     4081 aagtcttgcc aagtggcaca ctgtgcatct tctcgacaaa tcatttcaca ctggaagaat
     4141 tgtacaagac atgattgtcc tgtgtgtctc cccctcaaaa atgctggtga taagagaaat
     4201 caacagccaa ttttgactgg agcacccgtt ggacttggaa atcctagctc tctaggggtg
     4261 ggtcaacagt ctgcccccaa cctaagcact gttagtcaga ttgatcccag ctccatagaa
     4321 agagcctatg cagctcttgg actaccctat caagtaaatc agatgccgac acaaccccag
     4381 gtgcaagcaa agaaccagca gaatcagcag cctgggcagt ctccccaagg catgcggccc
     4441 atgagcaaca tgagtgctag tcctatggga gtaaatggag gtgtaggagt tcaaacgccg
     4501 agtcttcttt ctgactcaat gttgcattca gccataaatt ctcaaaaccc aatgatgagt
     4561 gaaaatgcca gtgtgccctc catgggtcct atgccaacag cagctcaacc atccactact
     4621 ggaattcgga aacagtggca cgaagatatt actcaggatc ttcgaaatca tcttgttcac
     4681 aaactcgtcc aagccatatt tcctacgccg gatcctgctg ctttaaaaga cagacggatg
     4741 gaaaacctag ttgcatatgc tcggaaagtt gaaggggaca tgtatgaatc tgcaaacaat
     4801 cgagcggaat actaccacct tctagctgag aaaatctata agatccagaa agaactagaa
     4861 gaaaaacgaa ggaccagact acagaagcag aacatgctac caaatgctgc aggcatggtt
     4921 ccagtttcca tgaatccagg gcctaacatg ggacagccgc aaccaggaat gacttctaat
     4981 ggccctctac ctgacccaag tatgatccgt ggcagtgtgc caaaccagat gatgcctcga
     5041 ataactccac aatctggttt gaatcaattt ggccagatga gcatggccca gccccctatt
     5101 gtaccccggc aaacccctcc tcttcagcac catggacagt tggctcaacc tggagctctc
     5161 aacccgccta tgggctatgg gcctcgtatg caacagcctt ccaaccaggg ccagttcctt
     5221 cctcagactc agttcccatc acagggaatg aatgtaacaa atatcccttt ggctccgtcc
     5281 agcggtcaag ctccagtgtc tcaagcacaa atgtctagtt cttcctgccc ggtgaactct
     5341 cctataatgc ctccagggtc tcaggggagc cacattcact gtccccagct tcctcaacca
     5401 gctcttcatc agaattcacc ctcgcctgta cctagtcgta cccccacccc tcaccatact
     5461 cccccaagca taggggctca gcagccacca gcaacaacaa ttccagcccc tgttcctaca
     5521 cctcctgcca tgccacctgg gccacagtcc caggctctac atccccctcc aaggcagaca
     5581 cctacaccac caacaacaca acttccccaa caagtgcagc cttcacttcc tgctgcacct
     5641 tctgctgacc agccccagca gcagcctcgc tcacagcaga gcacagcagc gtctgttcct
     5701 accccaacag caccgctgct tcctccgcag cctgcaactc cactttccca gccagctgta
     5761 agcattgaag gacaggtatc aaatcctcca tctactagta gcacagaagt gaattctcag
     5821 gccattgctg agaagcagcc ttcccaggaa gtgaagatgg aggccaaaat ggaagtggat
     5881 caaccagaac cagcagatac tcagccggag gatatttcag agtctaaagt ggaagactgt
     5941 aaaatggaat ctaccgaaac agaagagaga agcactgagt taaaaactga aataaaagag
     6001 gaggaagacc agccaagtac ttcagctacc cagtcatctc cggctccagg acagtcaaag
     6061 aaaaagattt tcaaaccaga agaactacga caggcactga tgccaacttt ggaggcactt
     6121 taccgtcagg atccagaatc ccttcccttt cgtcaacctg tggaccctca gcttttagga
     6181 atccctgatt actttgatat tgtgaagagc cccatggatc tttctaccat taagaggaag
     6241 ttagacactg gacagtatca ggagccctgg cagtatgtcg atgatatttg gcttatgttc
     6301 aataatgcct ggttatataa ccggaaaaca tcacgggtat acaaatactg ctccaagctc
     6361 tctgaggtct ttgaacaaga aattgaccca gtgatgcaaa gccttggata ctgttgtggc
     6421 agaaagttgg agttctctcc acagacactg tgttgctacg gcaaacagtt gtgcacaata
     6481 cctcgtgatg ccacttacta cagttaccag aacaggtatc atttctgtga gaagtgtttc
     6541 aatgagatcc aaggggagag cgtttctttg ggggatgacc cttcccagcc tcaaactaca
     6601 ataaataaag aacaattttc caagagaaaa aatgacacac tggatcctga actgtttgtt
     6661 gaatgtacag agtgcggaag aaagatgcat cagatctgtg tccttcacca tgagatcatc
     6721 tggcctgctg gattcgtctg tgatggctgt ttaaagaaaa gtgcacgaac taggaaagaa
     6781 aataagtttt ctgctaaaag gttgccatct accagacttg gcacctttct agagaatcgt
     6841 gtgaatgact ttctgaggcg acagaatcac cctgagtcag gagaggtcac tgttagagta
     6901 gttcatgctt ctgacaaaac cgtggaagta aaaccaggca tgaaagcaag gtttgtggac
     6961 agtggagaga tggcagaatc ctttccatac cgaaccaaag ccctctttgc ctttgaagaa
     7021 attgatggtg ttgacctgtg cttctttggc atgcatgttc aagagtatgg ctctgactgc
     7081 cctccaccca accagaggag agtatacata tcttacctct atagtgttca tttcttccgt
     7141 cctaaatgct tgaggactgc agtctatcat gaaatcctaa ttggatattt agaatatgtc
     7201 aagaaattag gttacacaac agggcatatt tgggcatgtc caccaagtga gggagatgat
     7261 tatatcttcc attgccatcc tcctgaccag aagataccca agcccaagcg actgcaggaa
     7321 tggtacaaaa aaatgcttga caaggctgta tcagagcgta ttgtccatga ctacaaggat
     7381 atttttaaac aagctactga agatagatta acaagtgcaa aggaattgcc ttatttcgag
     7441 ggtgatttct ggcccaatgt tctggaagaa agcattaagg aactggaaca ggaggaagaa
     7501 gagagaaaac gagaggaaaa caccagcaat gaaagcacag atgtgaccaa gggagacagc
     7561 aaaaatgcta aaaagaagaa taataagaaa accagcaaaa ataagagcag cctgagtagg
     7621 ggcaacaaga agaaacccgg gatgcccaat gtatctaacg acctctcaca gaaactatat
     7681 gccaccatgg agaagcataa agaggtcttc tttgtgatcc gcctcattgc tggccctgct
     7741 gccaactccc tgcctcccat tgttgatcct gatcctctca tcccctgcga tctgatggat
     7801 ggtcgggatg cgtttctcac gctggcaagg gacaagcacc tggagttctc ttcactccga
     7861 agagcccagt ggtccaccat gtgcatgctg gtggagctgc acacgcagag ccaggaccgc
     7921 tttgtctaca cctgcaatga atgcaagcac catgtggaga cacgctggca ctgtactgtc
     7981 tgtgaggatt atgacttgtg tatcacctgc tataacacta aaaaccatga ccacaaaatg
     8041 gagaaactag gccttggctt agatgatgag agcaacaacc agcaggctgc agccacccag
     8101 agcccaggcg attctcgccg cctgagtatc cagcgctgca tccagtctct ggtccatgct
     8161 tgccagtgtc ggaatgccaa ttgctcactg ccatcctgcc agaagatgaa gcgggttgtg
     8221 cagcatacca agggttgcaa acggaaaacc aatggcgggt gccccatctg caagcagctc
     8281 attgccctct gctgctacca tgccaagcac tgccaggaga acaaatgccc ggtgccgttc
     8341 tgcctaaaca tcaagcagaa gctccggcag caacagctgc agcaccgact acagcaggcc
     8401 caaatgcttc gcaggaggat ggccagcatg cagcggactg gtgtggttgg gcagcaacag
     8461 ggcctccctt cccccactcc tgccactcca acgacaccaa ctggccaaca gccaaccacc
     8521 ccgcagacgc cccagcccac ttctcagcct cagcctaccc ctcccaatag catgccaccc
     8581 tacttgccca ggactcaagc tgctggccct gtgtcccagg gtaaggcagc aggccaggtg
     8641 acccctccaa cccctcctca gactgctcag ccaccccttc caggaccccc acctgcagca
     8701 gtggaaatgg caatgcagat tcagagagca gcggagacgc agcgccagat ggcccacgtg
     8761 caaatttttc aaaggccaat ccaacaccag atgcccccga tgactcccat ggcccccatg
     8821 ggtatgaacc cacctcccat gaccagaggt cccagtgggc atttggagcc agggatggga
     8881 ccgacaggga tgcagcaaca gccaccctgg agccaaggag gattgcctca gccccagcaa
     8941 ctacagtctg ggatgccaag gccagccatg atgtcagtgg cccagcatgg tcaacctttg
     9001 aacatggctc cacaaccagg attgggccag gtaggtatca gcccactcaa accaggcact
     9061 gtgtctcaac aagccttaca aaaccttttg cggactctca ggtctcccag ctctcccctg
     9121 cagcagcaac aggtgcttag tatccttcac gccaaccccc agctgttggc tgcattcatc
     9181 aagcagcggg ctgccaagta tgccaactct aatccacaac ccatccctgg gcagcctggc
     9241 atgccccagg ggcagccagg gctacagcca cctaccatgc caggtcagca gggggtccac
     9301 tccaatccag ccatgcagaa catgaatcca atgcaggcgg gcgttcagag ggctggcctg
     9361 ccccagcagc aaccacagca gcaactccag ccacccatgg gagggatgag cccccaggct
     9421 cagcagatga acatgaacca caacaccatg ccttcacaat tccgagacat cttgagacga
     9481 cagcaaatga tgcaacagca gcagcaacag ggagcagggc caggaatagg ccctggaatg
     9541 gccaaccata accagttcca gcaaccccaa ggagttggct acccaccaca gcagcagcag
     9601 cggatgcagc atcacatgca acagatgcaa caaggaaata tgggacagat aggccagctt
     9661 ccccaggcct tgggagcaga ggcaggtgcc agtctacagg cctatcagca gcgactcctt
     9721 cagcaacaga tggggtcccc tgttcagccc aaccccatga gcccccagca gcatatgctc
     9781 ccaaatcagg cccagtcccc acacctacaa ggccagcaga tccctaattc tctctccaat
     9841 caagtgcgct ctccccagcc tgtcccttct ccacggccac agtcccagcc cccccactcc
     9901 agtccttccc caaggatgca gcctcagcct tctccacacc acgtttcccc acagacaagt
     9961 tccccacatc ctggactggt agctgcccag gccaacccca tggaacaagg gcattttgcc
    10021 agcccggacc agaattcaat gctttctcag cttgctagca atccaggcat ggcaaacctc
    10081 catggtgcaa gcgccacgga cctgggactc agcaccgata actcagactt gaattcaaac
    10141 ctctcacaga gtacactaga catatgagcg gccgctcgag tcta
//
