LOCUS       sequence-402669-        8072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)
FEATURES             Location/Qualifiers
     source          1..8072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..39
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             43..747
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      6010..6019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagcttatgg gaccggctgc caagcgcgtg aaactcgatg gcgtgagcaa gggcgaggag
       61 gataacatgg cctctctccc agcgacacat gagttacaca tctttggctc catcaacggt
      121 gtggactttg acatggtggg tcagggcacc ggcaatccaa atgatggtta tgaggagtta
      181 aacctgaagt ccaccaaggg tgacctccag ttctccccct ggattctggt ccctcatatc
      241 gggtatggct tccatcagta cctgccctac cctgacggga tgtcgccttt ccaggccgca
      301 atggtagatg gctccggata ccaagtccat cgcacaatgc agtttgaaga tggtgcctcc
      361 cttactgtta actaccgcta cacctacgag ggaagccaca tcaaaggaga ggcccaggtg
      421 aaggggactg gtttccctgc tgacggtcct gtgatgacca actcgctgac cgctgcggac
      481 tggtgcaggt cgaagaagac ttaccccaac gacaaaacca tcatcagtac ctttaagtgg
      541 agttacacca ctggaaatgg caagcgctac cggagcactg cgcggaccac ctacaccttt
      601 gccaagccaa tggcggctaa ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg
      661 gaactcaagc actccaagac cgaactcaac ttcaaggagt ggcaaaaggc ctttaccgat
      721 gtgatgggca tggacgagct gtacaagggc ggctcgtacg tgtacaagtc tgcggagagc
      781 ggccctggga cgagattgag aaatctgcca gtaatggggg atggactaga aacttcccaa
      841 atgtctacaa cacaggccca ggcccaaccc cagccagcca acgcagccag caccaacccc
      901 ccgcccccag agacctccaa ccctaacaag cccaagaggc agaccaacca actgcaatac
      961 ctgctcagag tggtgctcaa gacactatgg aaacaccagt ttgcatggcc tttccagcag
     1021 cctgtggatg ccgtcaagct gaacctccct gattactata agatcattaa aacgcctatg
     1081 gatatgggaa caataaagaa gcgcttggaa aacaactatt actggaatgc tcaggaatgt
     1141 atccaggact tcaacactat gtttacaaat tgttacatct acaacaagcc tggagatgac
     1201 atagtcttaa tggcagaagc tctggaaaag ctcttcttgc aaaaaataaa tgagctaccc
     1261 acagaagaaa ccgagatcat gatagtccag gcaaaaggaa gaggacgtgg gaggaaagaa
     1321 acagggacag caaaacctgg cgtttccacg gtaccaaaca caactcaagc atcgactcct
     1381 ccgcagaccc agacccctca gccgaatcct cctcctgtgc aggccacgcc tcaccccttc
     1441 cctgccgtca ccccggacct catcgtccag acccctgtca tgacagtggt gcctccccag
     1501 ccactgcaga cgcccccgcc agtgcccccc cagccacaac ccccacccgc tccagctccc
     1561 cagcccgtac agagccaccc acccatcatc gcggccaccc cacagcctgt gaagacaaag
     1621 aagggagtga agaggaaagc agacaccacc acccccacca ccattgaccc cattcacgag
     1681 ccaccctcgc tgcccccgga gcccaagacc accaagctgg gccagcggcg ggagagcagc
     1741 cggcctgtga aacctccaaa gaaggacgtg cccgactctc agcagcaccc agcaccagag
     1801 aagagcagca aggtctcgga gcagctcaag tgctgcagcg gcatcctcaa ggagatgttt
     1861 gccaagaagc acgccgccta cgcctggccc ttctacaagc ctgtggacgt ggaggcactg
     1921 ggcctacacg actactgtga catcatcaag caccccatgg acatgagcac aatcaagtct
     1981 aaactggagg cccgtgagta ccgtgatgct caggagtttg gtgctgacgt ccgattgatg
     2041 ttctccaact gctataagta caaccctcct gaccatgagg tggtggccat ggcccgcaag
     2101 ctccaggatg tgttcgaaat gcgctttgcc aagatgccgg acgagcctga ggagccagtg
     2161 gtggccgtgt cctccccggc agtgccccct cccaccaagg ttgtggcccc gccctcatcc
     2221 agcgacagca gcagcgatag ctcctcggac agtgacagtt cgactgatga ctctgaggag
     2281 gagcgagccc agcggctggc tgagctccag gagcagctca aagccgtgca cgagcagctt
     2341 gcagccctct ctcagcccca gcagaacaaa ccaaagaaaa aggagaaaga caagaaggaa
     2401 aagaaaaaag aaaagcacaa aaggaaagag gaagtggaag agaataaaaa aagcaaagcc
     2461 aaggaacctc ctcctaaaaa gacgaagaaa aataatagca gcaacagcaa tgtgagcaag
     2521 aaggagccag cgcccatgaa gagcaagccc cctcccacgt atgagtcgga ggaagaggac
     2581 aagtgcaagc ctatgtccta tgaggagaag cggcagctca gcttggacat caacaagctc
     2641 cccggcgaga agctgggccg cgtggtgcac atcatccagt cacgggagcc ctccctgaag
     2701 aattccaacc ccgacgagat tgaaatcgac tttgagaccc tgaagccgtc cacactgcgt
     2761 gagctggagc gctatgtcac ctcctgtttg cggaagaaaa ggaaacctca agctgagaaa
     2821 gttgatgtga ttgccggctc ctccaagatg aagggcttct cgtcctcaga gtcggagagc
     2881 tccagtgagt ccagctcctc tgacagcgaa gactccgaaa caatggccga gaatgtggtg
     2941 gaaccggggc cgccttcagc caagcggcct aaactctcat ctccggccct ctcggcgtcc
     3001 gccagcgatg gcacagattt tggctctcta tttgacttgg agcacgactt accagatgaa
     3061 ttaatcaact ctacagaatt gggactaacc aatggtggtg atattaatca gcttcagaca
     3121 agtcttggca tggtacaaga tgcagcttct aaacataaac agctgtcaga attgctgcga
     3181 tctggtagtt cccctaacct caatatggga gttggtggcc caggtcaagt catggccagc
     3241 caggcccaac agagcagtcc tggattaggt ttgataaata gcatggtcaa aagcccaatg
     3301 acacaggcag gcttgacttc tcccaacatg gggatgggca ctagtggacc aaatcagggt
     3361 cctacgcagt caacaggtat gatgaacagt ccagtaaatc agcctgccat gggaatgaac
     3421 acagggatga atgcgggcat gaatcctgga atgttggctg caggcaatgg acaagggata
     3481 atgcctaatc aagtcatgaa cggttcaatt ggagcaggcc gagggcgaca gaatatgcag
     3541 tacccaaacc caggcatggg aagtgctggc aacttactga ctgagcctct tcagcagggc
     3601 tctccccaga tgggaggaca aacaggattg agaggccccc agcctcttaa gatgggaatg
     3661 atgaacaacc ccaatcctta tggttcacca tatactcaga atcctggaca gcagattgga
     3721 gccagtggcc ttggtctcca gattcagaca aaaactgtac tatcaaataa cttatctcca
     3781 tttgctatgg acaaaaaggc agttcctggt ggaggaatgc ccaacatggg tcaacagcca
     3841 gccccgcagg tccagcagcc aggtctggtg actccagttg cccaagggat gggttctgga
     3901 gcacatacag ctgaaaaacg aaggaccaga ctacagaagc agaacatgct accaaatgct
     3961 gcaggcatgg ttccagtttc catgaatcca gggcctaaca tgggacagcc gcaaccagga
     4021 atgacttcta atggccctct acctgaccca agtatgatcc gtggcagtgt gccaaaccag
     4081 atgatgcctc gaataactcc acaatctggt ttgaatcaat ttggccagat gagcatggcc
     4141 cagcccccta ttgtaccccg gcaaacccct cctcttcagc accatggaca gttggctcaa
     4201 cctggagctc tcaacccgcc tatgggctat gggcctcgta tgcaacagcc ttccaaccag
     4261 ggccagttcc ttcctcagac tcagttccca tcacagggaa tgaatgtaac aaatatccct
     4321 ttggctccgt ccagcggtca agctccagtg tctcaagcac aaatgtctag ttcttcctgc
     4381 ccggtgaact ctcctataat gcctccaggg tctcagggga gccacattca ctgtccccag
     4441 cttcctcaac cagctcttca tcagaattca ccctcgcctg tacctagtcg tacccccacc
     4501 cctcaccata ctcccccaag cataggggct cagcagccac cagcaacaac aattccagcc
     4561 cctgttccta cacctcctgc catgccacct gggccacagt cccaggctct acatccccct
     4621 ccaaggcaga cacctacacc accaacaaca caacttcccc aacaagtgca gccttcactt
     4681 cctgctgcac cttctgctga ccagccccag cagcagcctc gctcacagca gagcacagca
     4741 gcgtctgttc ctaccccaac agcaccgctg cttcctccgc agcctgcaac tccactttcc
     4801 cagccagctg taagcattga aggacaggta tcaaatcctc catctactag tagcacagaa
     4861 gtgaattctc aggccattgc tgagaagcag ccttcccagg aagtgaagat ggaggccaaa
     4921 atggaagtgg atcaaccaga accagcagat actcagccgg aggatatttc agagtctaaa
     4981 gtggaagact gtaaaatgga atctaccgaa acagaagaga gaagcactga gttaaaaact
     5041 gaaataaaag aggaggaaga ccagccaagt acttcagcta cccagtcatc tccggctcca
     5101 ggacagtcaa agaaaaagtc tgctaaaagg ttgccatcta ccagacttgg cacctttcta
     5161 gagaatcgtg tgaatgactt tctgaggcga cagaatcacc ctgagtcagg agaggtcact
     5221 gttagagtag ttcatgcttc tgacaaaacc gtggaagtaa aaccaggcat gaaagcaagg
     5281 tttgtggaca gtggagagat ggcagaatcc tttccatacc gaaccaaagc cctctttgcc
     5341 tttgaagaaa ttgatggtgt tgacctgtgc ttctttggca tgcatgttca agagtatggc
     5401 tctgactgcc ctccacccaa ccagaggaga gtatacatat cttacctcga tagtgttcat
     5461 ttcttccgtc ctaaatgctt gaggactgca gtctatcatg aaatcctaat tggatattta
     5521 gaatatgtca agaaattagg ttacacaaca gggcatattt gggcatgtcc accaagtgag
     5581 ggagatgatt atatcttcca ttgccatcct cctgaccaga agatacccaa gcccaagcga
     5641 ctgcaggaat ggtacaaaaa aatgcttgac aaggctgtat cagagcgtat tgtccatgac
     5701 tacaaggata tttttaaaca agctactgaa gatagattaa caagtgcaaa ggaattgcct
     5761 tatttcgagg gtgatttctg gcccaatgtt ctggaagaaa gcattaagga actggaacag
     5821 gaggaagaag agagaaaacg agaggaaaac accagcaatg aaagcacaga tgtgaccaag
     5881 ggagacagca aaaatgctaa aaagaagaat aataagaaaa ccagcaaaaa taagagcagc
     5941 ctgagtaggg gcaacaagaa gaaacccggg atgcccaatg tatctaacga cctctcacag
     6001 aaactatatg ccaccatgga gaagcataaa gaggtcttct ttgtgatccg cctcattgct
     6061 ggccctgctg ccaactccct gcctcccatt gttgatcctg atcctctcat cccctgcgat
     6121 ctgatggatg gtcgggatgc gtttctcacg ctggcaaggg acaagcacct ggagttctct
     6181 tcactccgaa gagcccagtg gtccaccatg tgcatgctgg tggagctgca cacgcagagc
     6241 cagcagaagc tccggcagca acagctgcag caccgactac agcaggccca aatgcttcgc
     6301 aggaggatgg ccagcatgca gcggactggt gtggttgggc agcaacaggg cctcccttcc
     6361 cccactcctg ccactccaac gacaccaact ggccaacagc caaccacccc gcagacgccc
     6421 cagcccactt ctcagcctca gcctacccct cccaatagca tgccacccta cttgcccagg
     6481 actcaagctg ctggccctgt gtcccagggt aaggcagcag gccaggtgac ccctccaacc
     6541 cctcctcaga ctgctcagcc accccttcca gggcccccac ctgcagcagt ggaaatggca
     6601 atgcagattc agagagcagc ggagacgcag cgccagatgg cccacgtgca aatttttcaa
     6661 aggccaatcc aacaccagat gcccccgatg actcccatgg cccccatggg tatgaaccca
     6721 cctcccatga ccagaggtcc cagtgggcat ttggagccag ggatgggacc gacagggatg
     6781 cagcaacagc caccctggag ccaaggagga ttgcctcagc cccagcaact acagtctggg
     6841 atgccaaggc cagccatgat gtcagtggcc cagcatggtc aacctttgaa catggctcca
     6901 caaccaggat tgggccaggt aggtatcagc ccactcaaac caggcactgt gtctcaacaa
     6961 gccttacaaa accttttgcg gactctcagg tctcccagct ctcccctgca gcagcaacag
     7021 gtgcttagta tccttcacgc caacccccag ctgttggctg cattcatcaa gcagcgggct
     7081 gccaagtatg ccaactctaa tccacaaccc atccctgggc agcctggcat gccccagggg
     7141 cagccagggc tacagccacc taccatgcca ggtcagcagg gggtccactc caatccagcc
     7201 atgcagaaca tgaatccaat gcaggcgggc gttcagaggg ctggcctgcc ccagcagcaa
     7261 ccacagcagc aactccagcc acccatggga gggatgagcc cccaggctca gcagatgaac
     7321 atgaaccaca acaccatgcc ttcacaattc cgagacatct tgagacgaca gcaaatgatg
     7381 caacagcagc agcaacaggg agcagggcca ggaataggcc ctggaatggc caaccataac
     7441 cagttccagc aaccccaagg agttggctac ccaccacagc agcagcagcg gatgcagcat
     7501 cacatgcaac agatgcaaca aggaaatatg ggacagatag gccagcttcc ccaggccttg
     7561 ggagcagagg caggtgccag tctacaggcc tatcagcagc gactccttca gcaacagatg
     7621 gggtcccctg ttcagcccaa ccccatgagc ccccagcagc atatgctccc aaatcaggcc
     7681 cagtccccac acctacaagg ccagcagatc cctaattctc tctccaatca agtgcgctct
     7741 ccccagcctg tcccttctcc acggccacag tcccagcccc cccactccag tccttcccca
     7801 aggatgcagc ctcagccttc tccacaccac gtttccccac agacaagttc cccacatcct
     7861 ggactggtag ctgcccaggc caaccccatg gaacaagggc attttgccag cccggaccag
     7921 aattcaatgc tttctcagct tgctagcaat ccaggcatgg caaacctcca tggtgcaagc
     7981 gccacggacc tgggactcag caccgataac tcagacttga attcaaacct ctcacagagt
     8041 acactagaca tatgagcggc cgctcgagtc ta
//
