LOCUS       sequence-402670-        6952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)
FEATURES             Location/Qualifiers
     source          1..6952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..44
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             48..752
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
ORIGIN
        1 aacttaagct tatgggaccg gctgccaagc gcgtgaaact cgatggcgtg agcaagggcg
       61 aggaggataa catggcctct ctcccagcga cacatgagtt acacatcttt ggctccatca
      121 acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg
      181 agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc
      241 atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg
      301 ccgcaatggt agatggctcc ggataccaag tccatcgcac aatgcagttt gaagatggtg
      361 cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc
      421 aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg
      481 cggactggtg caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta
      541 agtggagtta caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca
      601 cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta
      661 agacggaact caagcactcc aagaccgaac tcaacttcaa ggagtggcaa aaggccttta
      721 ccgatgtgat gggcatggac gagctgtaca agggcggctc gtacgtgtac aagtctgcgg
      781 agagcggccc tgggacgaga ttgagaaatc tgccagtaat gggggatgga ctagaaactt
      841 cccaaatgtc tacaacacag gcccaggccc aaccccagcc agccaacgca gccagcacca
      901 accccccgcc cccagagacc tccaacccta acaagcccaa gaggcagacc aaccaactgc
      961 aatacctgct cagagtggtg ctcaagacac tatggaaaca ccagtttgca tggcctttcc
     1021 agcagcctgt ggatgccgtc aagctgaacc tccctgatta ctataagatc attaaaacgc
     1081 ctatggatat gggaacaata aagaagcgct tggaaaacaa ctattactgg aatgctcagg
     1141 aatgtatcca ggacttcaac actatgttta caaattgtta catctacaac aagcctggag
     1201 atgacatagt cttaatggca gaagctctgg aaaagctctt cttgcaaaaa ataaatgagc
     1261 tacccacaga agaaaccgag atcatgatag tccaggcaaa aggaagagga cgtgggagga
     1321 aagaaacagg gacagcaaaa cctggcgttt ccacggtacc aaacacaact caagcatcga
     1381 ctcctccgca gacccagacc cctcagccga atcctcctcc tgtgcaggcc acgcctcacc
     1441 ccttccctgc cgtcaccccg gacctcatcg tccagacccc tgtcatgaca gtggtgcctc
     1501 cccagccact gcagacgccc ccgccagtgc ccccccagcc acaaccccca cccgctccag
     1561 ctccccagcc cgtacagagc cacccaccca tcatcgcggc caccccacag cctgtgaaga
     1621 caaagaaggg agtgaagagg aaagcagaca ccaccacccc caccaccatt gaccccattc
     1681 acgagccacc ctcgctgccc ccggagccca agaccaccaa gctgggccag cggcgggaga
     1741 gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga ctctcagcag cacccagcac
     1801 cagagaagag cagcaaggtc tcggagcagc tcaagtgctg cagcggcatc ctcaaggaga
     1861 tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta caagcctgtg gacgtggagg
     1921 cactgggcct acacgactac tgtgacatca tcaagcaccc catggacatg agcacaatca
     1981 agtctaaact ggaggcccgt gagtaccgtg atgctcagga gtttggtgct gacgtccgat
     2041 tgatgttctc caactgctat aagtacaacc ctcctgacca tgaggtggtg gccatggccc
     2101 gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat gccggacgag cctgaggagc
     2161 cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg gccccgccct
     2221 catccagcga cagcagcagc gatagctcct cggacagtga cagttcgact gatgactctg
     2281 aggaggagcg agcccagcgg ctggctgagc tccaggagca gctcaaagcc gtgcacgagc
     2341 agcttgcagc cctctctcag ccccagcaga acaaaccaaa gaaaaaggag aaagacaaga
     2401 aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt ggaagagaat aaaaaaagca
     2461 aagccaagga acctcctcct aaaaagacga agaaaaataa tagcagcaac agcaatgtga
     2521 gcaagaagga gccagcgccc atgaagagca agccccctcc cacgtatgag tcggaggaag
     2581 aggacaagtg caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca
     2641 agctccccgg cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc
     2701 tgaagaattc caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac
     2761 tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg
     2821 agaaagttga tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg
     2881 agagctccag tgagtccagc tcctctgaca gcgaagactc cgaaacaatg gccgagaatg
     2941 tggtggaacc ggggccgcct tcagccaagc ggcctaaact ctcatctccg gccctctcgg
     3001 cgtccgccag cgatggcaca gattttggct ctctatttga cttggagcac gacttaccag
     3061 atgaattaat caactctaca gaattgggac taaccaatgg tggtgatatt aatcagcttc
     3121 agacaagtct tggcatggta caagatgcag cttctaaaca taaacagctg tcagaattgc
     3181 tgcgatctgg tagttcccct aacctcaata tgggagttgg tggcccaggt caagtcatgg
     3241 ccagccaggc ccaacagagc agtcctggat taggtttgat aaatagcatg gtcaaaagcc
     3301 caatgacaca ggcaggcttg acttctccca acatggggat gggcactagt ggaccaaatc
     3361 agggtcctac gcagtcaaca ggtatgatga acagtccagt aaatcagcct gccatgggaa
     3421 tgaacacagg gatgaatgcg ggcatgaatc ctggaatgtt ggctgcaggc aatggacaag
     3481 ggataatgcc taatcaagtc atgaacggtt caattggagc aggccgaggg cgacagaata
     3541 tgcagtaccc aaacccaggc atgggaagtg ctggcaactt actgactgag cctcttcagc
     3601 agggctctcc ccagatggga ggacaaacag gattgagagg cccccagcct cttaagatgg
     3661 gaatgatgaa caaccccaat ccttatggtt caccatatac tcagaatcct ggacagcaga
     3721 ttggagccag tggccttggt ctccagattc agacaaaaac tgtactatca aataacttat
     3781 ctccatttgc tatggacaaa aaggcagttc ctggtggagg aatgcccaac atgggtcaac
     3841 agccagcccc gcaggtccag cagccaggtc tggtgactcc agttgcccaa gggatgggtt
     3901 ctggagcaca tacagctgaa aaacgaagga ccagactaca gaagcagaac atgctaccaa
     3961 atgctgcagg catggttcca gtttccatga atccagggcc taacatggga cagccgcaac
     4021 caggaatgac ttctaatggc cctctacctg acccaagtat gatccgtggc agtgtgccaa
     4081 accagatgat gcctcgaata actccacaat ctggtttgaa tcaatttggc cagatgagca
     4141 tggcccagcc ccctattgta ccccggcaaa cccctcctct tcagcaccat ggacagttgg
     4201 ctcaacctgg agctctcaac ccgcctatgg gctatgggcc tcgtatgcaa cagccttcca
     4261 accagggcca gttccttcct cagactcagt tcccatcaca gggaatgaat gtaacaaata
     4321 tccctttggc tccgtccagc ggtcaagctc cagtgtctca agcacaaatg tctagttctt
     4381 cctgcccggt gaactctcct ataatgcctc cagggtctca ggggagccac attcactgtc
     4441 cccagcttcc tcaaccagct cttcatcaga attcaccctc gcctgtacct agtcgtaccc
     4501 ccacccctca ccatactccc ccaagcatag gggctcagca gccaccagca acaacaattc
     4561 cagcccctgt tcctacacct cctgccatgc cacctgggcc acagtcccag gctctacatc
     4621 cccctccaag gcagacacct acaccaccaa caacacaact tccccaacaa gtgcagcctt
     4681 cacttcctgc tgcaccttct gctgaccagc cccagcagca gcctcgctca cagcagagca
     4741 cagcagcgtc tgttcctacc ccaacagcac cgctgcttcc tccgcagcct gcaactccac
     4801 tttcccagcc agctgtaagc attgaaggac aggtatcaaa tcctccatct actagtagca
     4861 cagaagtgaa ttctcaggcc attgctgaga agcagccttc ccaggaagtg aagatggagg
     4921 ccaaaatgga agtggatcaa ccagaaccag cagatactca gccggaggat atttcagagt
     4981 ctaaagtgga agactgtaaa atggaatcta ccgaaacaga agagagaagc actgagttaa
     5041 aaactgaaat aaaagaggag gaagaccagc caagtacttc agctacccag tcatctccgg
     5101 ctccaggaca gtcaaagaaa aagcagaagc tccggcagca acagctgcag caccgactac
     5161 agcaggccca aatgcttcgc aggaggatgg ccagcatgca gcggactggt gtggttgggc
     5221 agcaacaggg cctcccttcc cccactcctg ccactccaac gacaccaact ggccaacagc
     5281 caaccacccc gcagacgccc cagcccactt ctcagcctca gcctacccct cccaatagca
     5341 tgccacccta cttgcccagg actcaagctg ctggccctgt gtcccagggt aaggcagcag
     5401 gccaggtgac ccctccaacc cctcctcaga ctgctcagcc accccttcca gggcccccac
     5461 ctgcagcagt ggaaatggca atgcagattc agagagcagc ggagacgcag cgccagatgg
     5521 cccacgtgca aatttttcaa aggccaatcc aacaccagat gcccccgatg actcccatgg
     5581 cccccatggg tatgaaccca cctcccatga ccagaggtcc cagtgggcat ttggagccag
     5641 ggatgggacc gacagggatg cagcaacagc caccctggag ccaaggagga ttgcctcagc
     5701 cccagcaact acagtctggg atgccaaggc cagccatgat gtcagtggcc cagcatggtc
     5761 aacctttgaa catggctcca caaccaggat tgggccaggt aggtatcagc ccactcaaac
     5821 caggcactgt gtctcaacaa gccttacaaa accttttgcg gactctcagg tctcccagct
     5881 ctcccctgca gcagcaacag gtgcttagta tccttcacgc caacccccag ctgttggctg
     5941 cattcatcaa gcagcgggct gccaagtatg ccaactctaa tccacaaccc atccctgggc
     6001 agcctggcat gccccagggg cagccagggc tacagccacc taccatgcca ggtcagcagg
     6061 gggtccactc caatccagcc atgcagaaca tgaatccaat gcaggcgggc gttcagaggg
     6121 ctggcctgcc ccagcagcaa ccacagcagc aactccagcc acccatggga gggatgagcc
     6181 cccaggctca gcagatgaac atgaaccaca acaccatgcc ttcacaattc cgagacatct
     6241 tgagacgaca gcaaatgatg caacagcagc agcaacaggg agcagggcca ggaataggcc
     6301 ctggaatggc caaccataac cagttccagc aaccccaagg agttggctac ccaccacagc
     6361 agcagcagcg gatgcagcat cacatgcaac agatgcaaca aggaaatatg ggacagatag
     6421 gccagcttcc ccaggccttg ggagcagagg caggtgccag tctacaggcc tatcagcagc
     6481 gactccttca gcaacagatg gggtcccctg ttcagcccaa ccccatgagc ccccagcagc
     6541 atatgctccc aaatcaggcc cagtccccac acctacaagg ccagcagatc cctaattctc
     6601 tctccaatca agtgcgctct ccccagcctg tcccttctcc acggccacag tcccagcccc
     6661 cccactccag tccttcccca aggatgcagc ctcagccttc tccacaccac gtttccccac
     6721 agacaagttc cccacatcct ggactggtag ctgcccaggc caaccccatg gaacaagggc
     6781 attttgccag cccggaccag aattcaatgc tttctcagct tgctagcaat ccaggcatgg
     6841 caaacctcca tggtgcaagc gccacggacc tgggactcag caccgataac tcagacttga
     6901 attcgaacct ctcacagagt acactagaca tatgagcggc cgctcgagtc ta
//
