LOCUS       sequence-402671-        6192 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)
FEATURES             Location/Qualifiers
     source          1..6192
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..41
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             45..749
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             3870..5720
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5484..5493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaagcttat gggaccggct gccaagcgcg tgaaactcga tggcgtgagc aagggcgagg
       61 aggataacat ggcctctctc ccagcgacac atgagttaca catctttggc tccatcaacg
      121 gtgtggactt tgacatggtg ggtcagggca ccggcaatcc aaatgatggt tatgaggagt
      181 taaacctgaa gtccaccaag ggtgacctcc agttctcccc ctggattctg gtccctcata
      241 tcgggtatgg cttccatcag tacctgccct accctgacgg gatgtcgcct ttccaggccg
      301 caatggtaga tggctccgga taccaagtcc atcgcacaat gcagtttgaa gatggtgcct
      361 cccttactgt taactaccgc tacacctacg agggaagcca catcaaagga gaggcccagg
      421 tgaaggggac tggtttccct gctgacggtc ctgtgatgac caactcgctg accgctgcgg
      481 actggtgcag gtcgaagaag acttacccca acgacaaaac catcatcagt acctttaagt
      541 ggagttacac cactggaaat ggcaagcgct accggagcac tgcgcggacc acctacacct
      601 ttgccaagcc aatggcggct aactatctga agaaccagcc gatgtacgtg ttccgtaaga
      661 cggaactcaa gcactccaag accgaactca acttcaagga gtggcaaaag gcctttaccg
      721 atgtgatggg catggacgag ctgtacaagg gcggctcgta cgtgtacaag tctgcggaga
      781 gcggccctgg gacgagattg agaaatctgc cagtaatggg ggatggacta gaaacttccc
      841 aaatgtctac aacacaggcc caggcccaac cccagccagc caacgcagcc agcaccaacc
      901 ccccgccccc agagacctcc aaccctaaca agcccaagag gcagaccaac caactgcaat
      961 acctgctcag agtggtgctc aagacactat ggaaacacca gtttgcatgg cctttccagc
     1021 agcctgtgga tgccgtcaag ctgaacctcc ctgattacta taagatcatt aaaacgccta
     1081 tggatatggg aacaataaag aagcgcttgg aaaacaacta ttactggaat gctcaggaat
     1141 gtatccagga cttcaacact atgtttacaa attgttacat ctacaacaag cctggagatg
     1201 acatagtctt aatggcagaa gctctggaaa agctcttctt gcaaaaaata aatgagctac
     1261 ccacagaaga aaccgagatc atgatagtcc aggcaaaagg aagaggacgt gggaggaaag
     1321 aaacagggac agcaaaacct ggcgtttcca cggtaccaaa cacaactcaa gcatcgactc
     1381 ctccgcagac ccagacccct cagccgaatc ctcctcctgt gcaggccacg cctcacccct
     1441 tccctgccgt caccccggac ctcatcgtcc agacccctgt catgacagtg gtgcctcccc
     1501 agccactgca gacgcccccg ccagtgcccc cccagccaca acccccaccc gctccagctc
     1561 cccagcccgt acagagccac ccacccatca tcgcggccac cccacagcct gtgaagacaa
     1621 agaagggagt gaagaggaaa gcagacacca ccacccccac caccattgac cccattcacg
     1681 agccaccctc gctgcccccg gagcccaaga ccaccaagct gggccagcgg cgggagagca
     1741 gccggcctgt gaaacctcca aagaaggacg tgcccgactc tcagcagcac ccagcaccag
     1801 agaagagcag caaggtctcg gagcagctca agtgctgcag cggcatcctc aaggagatgt
     1861 ttgccaagaa gcacgccgcc tacgcctggc ccttctacaa gcctgtggac gtggaggcac
     1921 tgggcctaca cgactactgt gacatcatca agcaccccat ggacatgagc acaatcaagt
     1981 ctaaactgga ggcccgtgag taccgtgatg ctcaggagtt tggtgctgac gtccgattga
     2041 tgttctccaa ctgctataag tacaaccctc ctgaccatga ggtggtggcc atggcccgca
     2101 agctccagga tgtgttcgaa atgcgctttg ccaagatgcc ggacgagcct gaggagccag
     2161 tggtggccgt gtcctccccg gcagtgcccc ctcccaccaa ggttgtggcc ccgccctcat
     2221 ccagcgacag cagcagcgat agctcctcgg acagtgacag ttcgactgat gactctgagg
     2281 aggagcgagc ccagcggctg gctgagctcc aggagcagct caaagccgtg cacgagcagc
     2341 ttgcagccct ctctcagccc cagcagaaca aaccaaagaa aaaggagaaa gacaagaagg
     2401 aaaagaaaaa agaaaagcac aaaaggaaag aggaagtgga agagaataaa aaaagcaaag
     2461 ccaaggaacc tcctcctaaa aagacgaaga aaaataatag cagcaacagc aatgtgagca
     2521 agaaggagcc agcgcccatg aagagcaagc cccctcccac gtatgagtcg gaggaagagg
     2581 acaagtgcaa gcctatgtcc tatgaggaga agcggcagct cagcttggac atcaacaagc
     2641 tccccggcga gaagctgggc cgcgtggtgc acatcatcca gtcacgggag ccctccctga
     2701 agaattccaa ccccgacgag attgaaatcg actttgagac cctgaagccg tccacactgc
     2761 gtgagctgga gcgctatgtc acctcctgtt tgcggaagaa aaggaaacct caagctgaga
     2821 aagttgatgt gattgccggc tcctccaaga tgaagggctt ctcgtcctca gagtcggaga
     2881 gctccagtga gtccagctcc tctgacagcg aagactccga aacagatcca gagaagcgca
     2941 agctcatcca gcagcagctt gttctccttt tgcatgctca caagtgccag cgccgggaac
     3001 aggccaatgg ggaagtgagg cagtgcaacc ttccccactg tcgcacaatg aagaatgtcc
     3061 taaaccacat gacacactgc cagtcaggca agtcttgcca agtggcacac tgtgcatctt
     3121 ctcgacaaat catttcacac tggaagaatt gtacaagaca tgattgtcct gtgtgtctcc
     3181 ccctcaaaaa tgctggtgat aagagaaatc aacagccaat tttgactgga gcacccgttg
     3241 gacttggaaa tcctagctct ctaggggtgg gtcaacagtc tgcccccaac ctaagcactg
     3301 ttagtcagat tgatcccagc tccatagaaa gagcctatgc agctcttgga ctaccctatc
     3361 aagtaaatca gatgccgaca caaccccagg tgcaagcaaa gaaccagcag aatcagcagc
     3421 ctgggcagtc tccccaaggc atgcggccca tgagcaacat gagtgctagt cctatgggag
     3481 taaatggagg tgtaggagtt caaacgccga gtcttctttc tgactcaatg ttgcattcag
     3541 ccataaattc tcaaaaccca atgatgagtg aaaatgccag tgtgccctcc atgggtccta
     3601 tgccaacagc agctcaacca tccactactg gaattcggaa acagtggcac gaagatatta
     3661 ctcaggatct tcgaaatcat cttgttcaca aactcgtcca agccatattt cctacgccgg
     3721 atcctgctgc tttaaaagac agacggatgg aaaacctagt tgcatatgct cggaaagttg
     3781 aaggggacat gtatgaatct gcaaacaatc gagcggaata ctaccacctt ctagctgaga
     3841 aaatctataa gatccagaaa gaactagaaa ttttcaaacc agaagaacta cgacaggcac
     3901 tgatgccaac tttggaggca ctttaccgtc aggatccaga atcccttccc tttcgtcaac
     3961 ctgtggaccc tcagctttta ggaatccctg attactttga tattgtgaag agccccatgg
     4021 atctttctac cattaagagg aagttagaca ctggacagta tcaggagccc tggcagtatg
     4081 tcgatgatat ttggcttatg ttcaataatg cctggttata taaccggaaa acatcacggg
     4141 tatacaaata ctgctccaag ctctctgagg tctttgaaca agaaattgac ccagtgatgc
     4201 aaagccttgg atactgttgt ggcagaaagt tggagttctc tccacagaca ctgtgttgct
     4261 acggcaaaca gttgtgcaca atacctcgtg atgccactta ttacagttac cagaacaggt
     4321 atcatttctg tgagaagtgt ttcaatgaga tccaagggga gagcgtttct ttgggggatg
     4381 acccttccca gcctcaaact acaataaata aagaacaatt ttccaagaga aaaaatgaca
     4441 cactggatcc tgaactgttt gttgaatgta cagagtgcgg aagaaagatg catcagatct
     4501 gtgtccttca ccatgagatc atctggcctg ctggattcgt ctgtgatggc tgtttaaaga
     4561 aaagtgcacg aactaggaaa gaaaataagt tttctgctaa aaggttgcca tctaccagac
     4621 ttggcacctt tctagagaat cgtgtgaatg actttctgag gcgacagaat caccctgagt
     4681 caggagaggt cactgttaga gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag
     4741 gcatgaaagc aaggtttgtg gacagtggag agatggcaga atcctttcca taccgaacca
     4801 aagccctctt tgcctttgaa gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg
     4861 ttcaagagta tggctctgac tgccctccac ccaaccagag gagagtatac atatcttacc
     4921 tcgatagtgt tcatttcttc cgtcctaaat gcttgaggac tgcagtctat catgaaatcc
     4981 taattggata tttagaatat gtcaagaaat taggttacac aacagggcat atttgggcat
     5041 gtccaccaag tgagggagat gattatatct tccattgcca tcctcctgac cagaagatac
     5101 ccaagcccaa gcgactgcag gaatggtaca aaaaaatgct tgacaaggct gtatcagagc
     5161 gtattgtcca tgactacaag gatattttta aacaagctac tgaagataga ttaacaagtg
     5221 caaaggaatt gccttatttc gagggtgatt tctggcccaa tgttctggaa gaaagcatta
     5281 aggaactgga acaggaggaa gaagagagaa aacgagagga aaacaccagc aatgaaagca
     5341 cagatgtgac caagggagac agcaaaaatg ctaaaaagaa gaataataag aaaaccagca
     5401 aaaataagag cagcctgagt aggggcaaca agaagaaacc cgggatgccc aatgtatcta
     5461 acgacctctc acagaaacta tatgccacca tggagaagca taaagaggtc ttctttgtga
     5521 tccgcctcat tgctggccct gctgccaact ccctgcctcc cattgttgat cctgatcctc
     5581 tcatcccctg cgatctgatg gatggtcggg atgcgtttct cacgctggca agggacaagc
     5641 acctggagtt ctcttcactc cgaagagccc agtggtccac catgtgcatg ctggtggagc
     5701 tgcacacgca gagccaggac cgctttgtct acacctgcaa tgaatgcaag caccatgtgg
     5761 agacacgctg gcactgtact gtctgtgagg attatgactt gtgtatcacc tgctataaca
     5821 ctaaaaacca tgaccacaaa atggagaaac taggccttgg cttagatgat gagagcaaca
     5881 accagcaggc tgcagccacc cagagcccag gcgattctcg ccgcctgagt atccagcgct
     5941 gcatccagtc tctggtccat gcttgccagt gtcggaatgc caattgctca ctgccatcct
     6001 gccagaagat gaagcgggtt gtgcagcata ccaagggttg caaacggaaa accaatggcg
     6061 ggtgccccat ctgcaagcag ctcattgccc tctgctgcta ccatgccaag cactgccagg
     6121 agaacaaatg cccggtgccg ttctgcctaa acatcaaggg ctcgacctga tcatagtgag
     6181 gctcgagtct ag
//
