LOCUS       sequence-402721-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To stably express mouse Kras encoded by native mouse codons and with
            a G12D mutation with an N-terminal FLAG tag in cells resistant to 
            Neo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Le Roux O, Pershing NLK, Kaltenbrun E, Newman NJ, Everitt JI, 
            Baldelli E, Pierobon M, Petricoin EF, Counter CM
  TITLE     Genetically manipulating endogenous Kras levels and oncogenic 
            mutations in vivo influences tissue patterning of murine 
            tumorigenesis.
  JOURNAL   Elife. 2022 Sep 7;11:e75715. doi: 10.7554/eLife.75715.
  PUBMED    36069770
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-7"; volume: "11:e75715. doi"; pages: " 
            10.7554/eLife.75715"
COMMENT     Sequence Label: pBabePuro-FLAG-KrasnatG12D
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatccctcga ggccgccacc atggactaca aagacgatga cgacaagatg actgagtata
       61 aacttgtggt ggttggagct gatggcgtag gcaagagcgc cttgacgata cagctaattc
      121 agaatcactt tgtggatgag tacgacccta cgatagagga ctcctacagg aaacaagtag
      181 taattgatgg agaaacctgt ctcttggata ttctcgacac agcaggtcaa gaggagtaca
      241 gtgcaatgag ggaccagtac atgagaactg gggagggctt tctttgtgta tttgccataa
      301 ataatactaa atcatttgaa gatattcacc attatagaga acaaattaaa agagtaaagg
      361 actctgaaga tgtgcctatg gtcctggtag ggaataagtg tgatttgcct tctagaacag
      421 tagacacgaa acaggctcag gagttagcaa ggagttacgg gattccgttc attgagacct
      481 cagcaaagac aagacagggt gttgacgatg ccttctatac attagtccga gaaattcgaa
      541 aacataaaga aaagatgagc aaagatggga agaagaagaa gaagaagtca aggacaaggt
      601 gtacagttat gtga
//
