LOCUS       sequence-402732-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of E276K VraS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - E276K
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(855..875)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             complement(891..908)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             933..955
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tacttagggg cctcgcttct ttcgggcttt gttagcagcc ggatcctcga gtcgacccgg
       61 gttaatcgtc atagctatcc ttctttgttc agcggcgcct taacctcaat acgggtaccg
      121 ctgtccggca ggctcacaat gtgaaaggtc gcgccgattt ccagcgcacg ctcacgcatg
      181 ttcttcagac catagctttg ttccagtttc tcatcaacgt taaagccctt gccgttgtcc
      241 tggatacgca gcagcaggta atcgtctttg ttgaacagct taacggtcac cttggtaccg
      301 ttgctgtgac gcagggtgtt gctaatcgct tcctgggtga tacgaaacag gtgatcctca
      361 atgcctttcg gcaccttgaa gtcttggatt tcgtgaacca ctttcatcgg aactttcttc
      421 tgcagatcaa tcaccaggtc cttgatgccc tcgcccaggc ttttatcctt cagacccagc
      481 ggacgcaggt gcagcagcag cgcacgcatt tccagttggc tatcctgaac cattttctcc
      541 agaatcggga tttgctggtc cagcggcggt tccagtttgg tctccttaat cgcgctcagc
      601 atcatgctcg ccgcgaacag ttgctggctc acgctgtcgt gcagctcacg cgccagacgt
      661 tgacgttcat cctcaatgat tttcttaacc ttcacgtcgt tgatgttgta gttttcgttg
      721 gtcaggtttt gggttttcag acgcagcttg tgcagctcct ggttcagcgg aaccagggtg
      781 tgatacagat ccagggtttc gctgtaaatc tcgatgttct ggtcgttgat gcccacggtt
      841 tcaccctcca tgcagctctg aaagtacaga tcctccatat ggccgctgct gtgatgatga
      901 tgatgatggc tgctgagctc gcccatggta tatctccttc ttaaagttaa acaaaattat
      961 ttctagaggg ga
//
