LOCUS       sequence-402733-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of L114S VraS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - L114S
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             27..49
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             74..91
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             107..127
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
ORIGIN
        1 tttggcgtaa ttccctctag aataattttg tttaacttta agaaggagat ataccatggg
       61 cgagctcagc agccatcatc atcatcatca cagcagcggc catatggagg atctgtactt
      121 tcagagctgc atggagggtg aaaccgtggg catcaacgac cagaacatcg agatttacag
      181 cgaaaccctg gatctgtatc acaccctggt tccgtcaaac caggagctgc acaagctgcg
      241 tctgaaaacc caaaacctga ccaacgaaaa ctacaacatc aacgacgtga aggttaagaa
      301 aatcattgag gatgaacgtc aacgtctggc gcgtgagctg cacgacagcg tgagccagca
      361 actgttcgcg gcgagcatga tgctgagcgc gattaaggag accaaactgg aaccgccgct
      421 ggaccagcaa atcccgattc tggagaaaat ggttcaggat agccaactgg aaatgcgtgc
      481 gctgctgctg cacctgcgtc cgctgggtct gaaggataaa agcctgggcg agggcatcaa
      541 ggacctggtg attgatctgc agaagaaagt tccgatgaaa gtggttcacg aaatccaaga
      601 cttcaaggtg ccgaaaggca ttgaggatca cctgtttcgt atcacccagg aagcgattag
      661 caacaccctg cgtcacagca acggtaccaa ggtgaccgtt gaactgttca acaaagacga
      721 ttacctgctg ctgcgtatcc aggacaacgg caagggcttt aacgttgatg agaaactgga
      781 acaaagctat ggtctgaaga acatgcgtga gcgtgcgctg gaaatcggcg cgacctttca
      841 cattgtgagc ctgccggaca gcggtacccg tagtgaggtt aggcgcagtg actaatatat
      901 agctatgatg attactcggg tcntttcggg atccgnnngc tagccatnag gaaggtacag
      961 tattggtcct gga
//
