LOCUS       sequence-402735-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of S329L VraS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - S329L
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             75..92
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..128
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
ORIGIN
        1 ccttggcgaa attccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg
       61 gcgagctcag cagccatcat catcatcatc acagcagcgg ccatatggag gatctgtact
      121 ttcagagctg catggagggt gaaaccgtgg gcatcaacga ccagaacatc gagatttaca
      181 gcgaaaccct ggatctgtat cacaccctgg ttccgctgaa ccaggagctg cacaagctgc
      241 gtctgaaaac ccaaaacctg accaacgaaa actacaacat caacgacgtg aaggttaaga
      301 aaatcattga ggatgaacgt caacgtctgg cgcgtgagct gcacgacagc gtgagccagc
      361 aactgttcgc ggcgagcatg atgctgagcg cgattaagga gaccaaactg gaaccgccgc
      421 tggaccagca aatcccgatt ctggagaaaa tggttcagga tagccaactg gaaatgcgtg
      481 cgctgctgct gcacctgcgt ccgctgggtc tgaaggataa aagcctgggc gagggcatca
      541 aggacctggt gattgatctg cagaagaaag ttccgatgaa agtggttcac gaaatccaag
      601 acttcaaggt gccgaaaggc attgaggatc acctgtttcg tatcacccag gaagcgatta
      661 gcaacaccct gcgtcacagc aacggtacca aggtgaccgt tgaactgttc aacaaagacg
      721 attacctgct gctgcgtatc caggacaacg gcaagggctt taacgttgat gagaaactgg
      781 aacaaagcta tggtctgaag aacatgcgtg agcgtgcgct ggaaatcggc gcgacctttc
      841 acattgtgag cctgccggac ttaggtaccc gtattgaggt taaggcgccg ctgaacaaag
      901 aagatagcta tgacgattaa cccgggtcga ctcgaggatc cggctgctaa caaagcccga
      961 aaggaagctg agttggctgc tgccacgctg accaataact agcataaccc cttggggccc
     1021 caaacgggtc tggaggtttt tgcggaaggg gacttttccg gatggagaag ggancccccc
     1081 gggnggggcc ataagcgcg
//
