LOCUS       sequence-402736-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of T331I VraS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - T331I
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..130
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
ORIGIN
        1 tgtggaggaa attccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg
       61 gcgagctcag ccagctcatc atcatcatca tcacagcagc ggccatatgg aggatctgta
      121 ctttcagagc tgcatggagg gtgaaaccgt gggcatcaac gaccagaaca tcgagattta
      181 cagcgaaacc ctggatctgt atcacaccct ggttccgctg aaccaggagc tgcacaagct
      241 gcgtctgaaa acccaaaacc tgaccaacga aaactacaac atcaacgacg tgaaggttaa
      301 gaaaatcatt gaggatgaac gtcaacgtct ggcgcgtgag ctgcacgaca gcgtgagcca
      361 gcaactgttc gcggcgagca tgatgctgag cgcgattaag gagaccaaac tggaaccgcc
      421 gctggaccag caaatcccga ttctggagaa aatggttcag gatagccaac tggaaatgcg
      481 tgcgctgctg ctgcacctgc gtccgctggg tctgaaggat aaaagcctgg gcgagggcat
      541 caaggacctg gtgattgatc tgcagaagaa agttccgatg aaagtggttc acgaaatcca
      601 agacttcaag gtgccgaaag gcattgagga tcacctgttt cgtatcaccc aggaagcgat
      661 tagcaacacc ctgcgtcaca gcaacggtac caaggtgacc gttgaactgt tcaacaaaga
      721 cgattacctg ctgctgcgta tccaggacaa cggcaagggc tttaacgttg atgagaaact
      781 ggaacaaagc tatggtctga agaacatgcg tgagcgtgcg ctggaaatcg gcgcgacctt
      841 tcacattgtg agcctgccgg acagcggtat ccgtattgag gttaaggcgc cgctgaacaa
      901 agaagatagc tatgacgatt aacccgggtc gactcgagga tccggctgct aacaaagccc
      961 gaaaggaagc tgagttggct gctgccaccg ctgagcataa ctagcataac cccttggggc
     1021 ctctaaacgg gtct
//
