LOCUS       sequence-402745-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of ALKBH5-Mut.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Shen C, Sheng Y, Zhu AC, Robinson S, Jiang X, Dong L, Chen H, Su R, 
            Yin Z, Li W, Deng X, Chen Y, Hu YC, Weng H, Huang H, Prince E, Cogle
            CR, Sun M, Zhang B, Chen CW, Marcucci G, He C, Qian Z, Chen J
  TITLE     RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer
            Stem Cell Self-Renewal in Acute Myeloid Leukemia.
  JOURNAL   Cell Stem Cell. 2020 Jul 2;27(1):64-80.e9. doi: 
            10.1016/j.stem.2020.04.009. Epub 2020 May 12.
  PUBMED    32402250
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2020.04.009"; journalName: "Cell Stem Cell"; date: 
            "2020-07-2- 2"; volume: "27"; issue: "1"; pages: "64-80.e9"
COMMENT     Sequence Label: PCDH-Mut-ALKBH5-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2287..2296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2296..2361
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3591..3802
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3815..4083
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4112..4121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4142..4804
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4874..4927
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4928..5527
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4928..4947)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5424..5444
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5528..6116
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5581..5601)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5999..6010)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6190..6423
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6495..6616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6532..6551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6586..6605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6656..6791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6824..6840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6824..6840)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6837..6859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6848..6864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6872..6878,6879..6896,6897..6902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6917..6938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7055..7072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7226..7814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7985..8776,8777..8845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8608..8627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8846..8950)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9018..9036
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9074..9096)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9196..9215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9409..9431
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9423..9440
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9424..9440
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcca ccatggatta caaagaccat gacggtgatt ataaagatca tgacatcgat
     2341 tacaaggatg acgatgacaa ggcggccgca tctggctata ccgatctgcg cgaaaaactg
     2401 aaaagtatga cgagccgcga taactacaaa gcgggtagcc gtgaagcggc cgcagcggcc
     2461 gcagcggccg tggcagccgc cgcagccgcc gcagcagcag cagaaccgta tccggttagc
     2521 ggcgcaaaac gcaaatacca ggaagatagc gatccggaac gttctgatta tgaagaacag
     2581 cagctgcaga aagaagaaga agcgcgtaaa gtgaaaagtg gcattcgcca gatgcgtctg
     2641 tttagccagg atgaatgcgc gaaaattgaa gcccgcatcg atgaagtggt tagccgtgcg
     2701 gaaaaaggtc tgtataacga acataccgtg gatcgcgccc cgctgcgtaa taaatacttt
     2761 ttcggcgaag gttatacgta cggtgcacag ctgcagaaac gtggtccggg tcaggaacgt
     2821 ctgtatccgc cgggtgatgt tgatgaaatt ccggaatggg tgcatcagct ggttatccag
     2881 aaactggtgg aacaccgtgt tattccggaa ggcttcgtga acagtgccgt tatcaatgat
     2941 taccagccgg gcggttgcat tgtgagcgct gttgatccga ttcacatctt tgaacgcccg
     3001 atcgtgtctg ttagcttttt cagcgattct gcactgtgct tcggttgtaa atttcagttc
     3061 aaaccgattc gtgtgagtga accggttctg agcctgccgg tgcgtcgcgg ttctgtgacc
     3121 gttctgagtg gttatgcggc cgatgaaatt acgcactgta tccgcccgca ggatatcaaa
     3181 gaacgtcgcg ccgtgattat cctgcgcaaa acccgtctgg atgcaccgcg tctggaaacg
     3241 aaaagcctga gctctagtgt tctgccgccg tcttacgcca gtgatcgcct gagcggtaac
     3301 aatcgtgatc cggcactgaa accgaaacgc tctcatcgta aagcagatcc ggatgcagca
     3361 caccgtccgc gtattctgga aatggataaa gaagaaaacc gtcgcagcgt gctgctgccg
     3421 acccatcgtc gccgtggttc ttttagctct gaaaattatt ggcgcaaaag ttacgaaagt
     3481 agcgaagatt gtagcgaagc cgcaggttct ccggcgcgta aagttaaaat gcgccgtcac
     3541 tgaggatccg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     3601 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     3661 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     3721 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     3781 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     3841 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     3901 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     3961 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     4021 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     4081 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct
     4141 gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct
     4201 ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac
     4261 caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta
     4321 ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg
     4381 cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt
     4441 cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt
     4501 ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca
     4561 cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg
     4621 cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca
     4681 ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca
     4741 cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt
     4801 cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc
     4861 caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     4921 cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     4981 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5041 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5101 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5161 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5221 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5281 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5341 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5401 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5461 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5521 tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5581 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5641 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5701 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5761 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5821 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5881 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5941 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6001 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6061 gcgtctccgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6121 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6181 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta
     6241 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6301 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6361 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6421 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6481 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6541 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6601 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6721 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6781 ttttggaggc ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg
     6841 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6901 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6961 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7021 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8881 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8941 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9001 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     9061 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9121 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9181 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     9241 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     9301 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     9361 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     9421 gttgtaaaac gacggccagt gccaagctg
//
