LOCUS       sequence-402746-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of ALKBH5-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Shen C, Sheng Y, Zhu AC, Robinson S, Jiang X, Dong L, Chen H, Su R, 
            Yin Z, Li W, Deng X, Chen Y, Hu YC, Weng H, Huang H, Prince E, Cogle
            CR, Sun M, Zhang B, Chen CW, Marcucci G, He C, Qian Z, Chen J
  TITLE     RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer
            Stem Cell Self-Renewal in Acute Myeloid Leukemia.
  JOURNAL   Cell Stem Cell. 2020 Jul 2;27(1):64-80.e9. doi: 
            10.1016/j.stem.2020.04.009. Epub 2020 May 12.
  PUBMED    32402250
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2020.04.009"; journalName: "Cell Stem Cell"; date: 
            "2020-07-2- 2"; volume: "27"; issue: "1"; pages: "64-80.e9"
COMMENT     Sequence Label: PCDH-WT-ALKBH5-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             223..403
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    450..575
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1068..1301
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1486..1530
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1797..1913
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2003..2206
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2157..2177
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2203..2227
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2277..2286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2286..2351
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3581..3792
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3805..4073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4102..4111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4132..4794
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4864..4917
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4918..5517
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4918..4937)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5414..5434
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5518..6106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5571..5591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5989..6000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6180..6413
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6485..6606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6522..6541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6576..6595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6646..6781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6814..6830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6814..6830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6827..6849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6838..6854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6862..6868,6869..6886,6887..6892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6907..6928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7045..7062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7216..7804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7975..8766,8767..8835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8598..8617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8836..8940)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9008..9026
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9064..9086)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9186..9205
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9399..9421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9413..9430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9414..9430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(9445..9449,1..222)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
       61 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      121 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      181 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      241 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      301 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      361 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      421 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      481 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      541 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      601 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      661 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      721 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
      781 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
      841 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
      901 ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag ggacaattgg
      961 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1021 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1081 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1141 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1201 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1261 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1321 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1381 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1441 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1501 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1561 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1621 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1681 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1741 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     1801 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1861 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata
     1921 ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1981 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2041 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2101 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2161 aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt tagtgaaccg
     2221 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat tctagagcca
     2281 ccatggatta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
     2341 acgatgacaa ggcggccgca tctggctata ccgatctgcg cgaaaaactg aaaagtatga
     2401 cgagccgcga taactacaaa gcgggtagcc gtgaagcggc cgcagcggcc gcagcggccg
     2461 tggcagccgc cgcagccgcc gcagcagcag cagaaccgta tccggttagc ggcgcaaaac
     2521 gcaaatacca ggaagatagc gatccggaac gttctgatta tgaagaacag cagctgcaga
     2581 aagaagaaga agcgcgtaaa gtgaaaagtg gcattcgcca gatgcgtctg tttagccagg
     2641 atgaatgcgc gaaaattgaa gcccgcatcg atgaagtggt tagccgtgcg gaaaaaggtc
     2701 tgtataacga acataccgtg gatcgcgccc cgctgcgtaa taaatacttt ttcggcgaag
     2761 gttatacgta cggtgcacag ctgcagaaac gtggtccggg tcaggaacgt ctgtatccgc
     2821 cgggtgatgt tgatgaaatt ccggaatggg tgcatcagct ggttatccag aaactggtgg
     2881 aacaccgtgt tattccggaa ggcttcgtga acagtgccgt tatcaatgat taccagccgg
     2941 gcggttgcat tgtgagccat gttgatccga ttcacatctt tgaacgcccg atcgtgtctg
     3001 ttagcttttt cagcgattct gcactgtgct tcggttgtaa atttcagttc aaaccgattc
     3061 gtgtgagtga accggttctg agcctgccgg tgcgtcgcgg ttctgtgacc gttctgagtg
     3121 gttatgcggc cgatgaaatt acgcactgta tccgcccgca ggatatcaaa gaacgtcgcg
     3181 ccgtgattat cctgcgcaaa acccgtctgg atgcaccgcg tctggaaacg aaaagcctga
     3241 gctctagtgt tctgccgccg tcttacgcca gtgatcgcct gagcggtaac aatcgtgatc
     3301 cggcactgaa accgaaacgc tctcatcgta aagcagatcc ggatgcagca caccgtccgc
     3361 gtattctgga aatggataaa gaagaaaacc gtcgcagcgt gctgctgccg acccatcgtc
     3421 gccgtggttc ttttagctct gaaaattatt ggcgcaaaag ttacgaaagt agcgaagatt
     3481 gtagcgaagc cgcaggttct ccggcgcgta aagttaaaat gcgccgtcac tgaggatccg
     3541 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     3601 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     3661 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     3721 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     3781 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     3841 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     3901 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     3961 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     4021 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     4081 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     4141 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     4201 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     4261 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     4321 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     4381 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     4441 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     4501 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     4561 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     4621 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     4681 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     4741 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     4801 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     4861 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     4921 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4981 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5041 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5101 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5161 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5221 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5281 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5341 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5401 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5461 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     5521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5881 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc
     6061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     6121 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6181 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     6241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6421 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6481 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6601 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     6661 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6721 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6781 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     6841 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6901 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6961 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7021 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7081 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7141 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7201 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7381 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7621 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7681 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7801 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7861 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7921 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7981 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8041 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8101 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8161 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8221 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8281 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8341 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8401 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8461 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8521 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8581 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8641 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8701 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8761 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8821 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8881 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8941 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9001 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     9061 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     9121 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     9181 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9241 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     9301 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     9361 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     9421 gacggccagt gccaagctga cgcgtgtag
//
