LOCUS       sequence-402750-        6115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo blocking of synaptic signaling through Tetanus toxin 
            mediated cleavage of synptobrevin proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR017
FEATURES             Location/Qualifiers
     source          1..6115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3860..3964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3965..4033,4034..4825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4183..4202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4996..5584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5485..5504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5738..5755
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5872..5893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5908..5913,5914..5931,5932..5938)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5946..5962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5951..5973
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5970..5986
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5970..5986
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt gcgatcgcta
      841 gcaaaaatgc cgatcaccat caacaacttc cgttactccg acccggttaa caacgacacc
      901 atcatcatga tggaaccgcc gtactgcaaa ggtctagaca tctactacaa agccttcaaa
      961 atcaccgacc gtatctggat cgttccggaa cgttacgaat ttggtactaa accggaagac
     1021 ttcaacccgc cgtcctccct gatcgaaggt gcttccgaat actacgaccc gaactacctg
     1081 cgtaccgact ccgacaaaga ccgtttcctg cagaccatgg ttaaactgtt caaccgtatc
     1141 aaaaacaacg ttgctggtga agctctgctg gacaaaatca tcaacgctat cccgtacctg
     1201 ggtaactcct actccctgct ggacaaattc gacaccaact ccaactccgt ttccttcaac
     1261 ctgctggaac aggatccgtc cggtgctacc accaaatccg ctatgctgac caacctgatc
     1321 atcttcggtc cgggtccggt tctgaacaaa aacgaagttc gtggtatcgt tctgcgtgtt
     1381 gacaacaaaa actacttccc gtgccgtgac ggtttcggtt ccatcatgca gatggctttc
     1441 tgcccggaat acgtaccgac cttcgacaac gttatcgaaa acatcacctc cctgaccatc
     1501 ggtaaatcca aatacttcca ggacccggct ctgctgctga tgcacgaact gatccacgtt
     1561 ctgcacggtc tgtacggcat gcaggtttcc tcccacgaaa tcatcccgtc caaacaggaa
     1621 atctacatgc agcacaccta cccgatctcc gctgaagagc tcttcacctt cggtggtcag
     1681 gacgctaacc tgatctccat cgatatcaaa aacgacctgt acgaaaaaac cctgaacgac
     1741 tacaaagcta tcgctaacaa actgtcccag gttacctcct gcaacgaccc gaacatcgac
     1801 atcgactcct acaaacagat ctatcagcag aagtatcagt ttgataagga ttccaacggc
     1861 cagtatatag tgaacgagga taagtttcag atactgtata actccataat gtatggcttt
     1921 actgagatag agctgggcaa gaagtttaac ataaagactc gcttaagcta tttttccatg
     1981 aaccacgatc ctgtgaagat acctaacctg ctggatgata ctatatataa cgatactgag
     2041 ggctttaaca tagagtccaa ggatctgaag tccgagtata agggccagaa catgagggtg
     2101 aacactaacg cctttaggaa cgtcgacggt tccggtctgg tttccaaact gatcggtctg
     2161 tgcaaaaaaa tcatcccgcc gaccaacatc cgtgaaaacc tgtacaaccg taccgcttaa
     2221 cctgcaggtt ttgaattcgc tgtctcatcc tactttcacc tagttaactg cttgtcttaa
     2281 aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta tataaatggt
     2341 ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt tcctcgtttt
     2401 tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct aattttaatt
     2461 cagggaaact gctgtaccgg tagaaaaaat ggtctccgag ctcattaaag aaaacatgca
     2521 tatgaagctc tacatggagg gaaccgtcaa caaccaccac ttcaagtgca cctccgaggg
     2581 agagggaaag ccatacgagg gaacccaaac catgcgtatc aaggccgtcg agggaggacc
     2641 actcccattc gccttcgaca tcctcgccac ctccttcatg tacggatcca aggtaagttt
     2701 aaacatatat atactaacta accctgatta tttaaatttt cagaccttca tcaaccacac
     2761 ccaaggaatc ccagacttct tcaagcaatc cttcccagag ggattcacct gggagcgtgt
     2821 caccacctac gaggacggag gagtcctcac cgccacccaa gacacctccc tccaagacgg
     2881 atgcctcatc tacaacgtca aggtaagttt aaacagttcg gtactaacta accatacata
     2941 tttaaatttt cagatccgtg gagtcaactt cccatccaac ggaccagtca tgcaaaagaa
     3001 gaccctcgga tgggaggcct ccaccgagac cctctaccca gccgacggag gacttgaggg
     3061 acgtgccgac atggccctca agctcgtcgg aggaggacac ctcatctgca acctcaaggt
     3121 aagtttaaac atgattttac taactaacta atctgattta aattttcaga ccacctaccg
     3181 ttccaagaag ccagccaaga acctcaagat gccaggagtc tactacgtcg accgtcgtct
     3241 tgagcgtatc aaggaggccg acaaggagac ctacgtcgag caacacgagg tcgccgtcgc
     3301 ccgttactgc gacctcccat ccaagctcgg acaccgttaa ttcgctagcc ggccatacaa
     3361 gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt
     3421 gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat
     3481 taattcaatc caaatctaaa catttttttt tctctttccg tctcccaatt cgtattccgc
     3541 tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga
     3601 cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga
     3661 cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata
     3721 aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga
     3781 tgagaattgt cctcgcttgg gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg
     3841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5761 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5821 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5881 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5941 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6001 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
     6061 aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat gaaat
//
