LOCUS       sequence-402755-       10645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: ADAR
FEATURES             Location/Qualifiers
     source          1..10645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3623..3647)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3772..3813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4268..4285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4274..4498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4631..4650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4782..4829
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4837..5859
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4837..5859
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5989..6110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6026..6045)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6080..6099
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6159..6175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6159..6175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6172..6194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6183..6199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6207..6213,6214..6231,6232..6237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6252..6273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6390..6407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6561..7149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6641..6660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7320..8111,8112..8180))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7943..7962
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8181..8285)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8360..8379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8667..9845
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8898..9836
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9793..9813
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     9862..9881
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9862..9880
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9959..9983
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccccaaact cagacccgag tttggaaccg gaagacagaa actccacatc tgtctcagaa
       61 gatcttcttg agccttttat tgcagtctca gctcaggctt ggaaccagca cagcggagtg
      121 gtaagaccag acagtcatag ccaaggatcc ccaaactcag acccaggttt ggaacctgaa
      181 gacagcaact ccacatctgc cttggaagat cctcttgagt ttttagacat ggccgagatc
      241 aaggagaaaa tctgcgacta tctcttcaat gtgtctgact cctctgccct gaatttggct
      301 aaaaatattg gccttaccaa ggcccgagat ataaatgctg tgctaattga catggaaagg
      361 cagggggatg tctatagaca agggacaacc cctcccatat ggcatttgac agcacatgat
      421 ctgtctggga aacttgggac tccgaagaaa gaaatcaatc gagttttata ctccctggca
      481 aagaagggca agctacagaa agaggcagga acaccccctt tgtggaaaat cgcggtctcc
      541 actcaggctt ggaaccagca cagcggagtg gtaagaccag acggtcatag ccaaggagcc
      601 ccaaactcag acccgagttt ggaaccggaa gacagaaact ccacatctgt ctcagaagat
      661 cttcttgagc cttttattgc agtctcagct caggcttgga accagcacag cggagtggta
      721 agaccagaca gtcatagcca aggatcccca aactcagacc caggtttgga acctgaagac
      781 agcaactcca catctgcctt ggaagatcct cttgagtttt tagacatggc cgagatcaag
      841 gagaaaatct gcgactatct cttcaatgtg tctgactcct ctgccctgaa tttggctaaa
      901 aatattggcc ttaccaaggc ccgagatata aatgctgtgc taattgacat ggaaaggcag
      961 ggggatgtct atagacaagg gacaacccct cccatatggc atttgacaga caagaagcga
     1021 gagaggatgc aaatcaagag aaatacgaac agtgttcctg aaaccgctcc agctgcaatc
     1081 cctgagacca aaagaaacgc agagttcctc acctgtaata tacccacatc aaatgcctca
     1141 aataacatgg taaccacaga aaaagtggag aatgggcagg aacctgtcat aaagttagaa
     1201 aacaggcaag aggccagacc agaaccagca agactgaaac cacctgttca ttacaatggc
     1261 ccctcaaaag cagggtatgt tgactttgaa aatggccagt gggccacaga tgacatccca
     1321 gatgacttga atagtatccg cgcagcacca ggtgagtttc gagccatcat ggagatgccc
     1381 tccttctaca gtcatggctt gccacggtgt tcaccctaca agaaactgac agagtgccag
     1441 ctgaagaacc ccatcagcgg gctgttagaa tatgcccagt tcgctagtca aacctgtgag
     1501 ttcaacatga tagagcagag tggaccaccc catgaacctc gatttaaatt ccaggttgtc
     1561 atcaatggcc gagagtttcc cccagctgaa gctggaagca agaaagtggc caagcaggat
     1621 gcagctatga aagccatgac aattctgcta gaggaagcca aagccaagga cagtggaaaa
     1681 tcagaagaat catcccacta ttccacagag aaagaatcag agaagactgc agagtcccag
     1741 acccccaccc cttcagccac atccttcttt tctgggaaga gccccgtcac cacactgctt
     1801 gagtgtatgc acaaattggg gaactcctgc gaattccgtc tcctgtccaa agaaggccct
     1861 gcccatgaac ccaagttcca atactgtgtt gcagtgggag cccaaacttt ccccagtgtg
     1921 agtgctccca gcaagaaagt ggcaaagcag atggccgcag aggaagccat gaaggccctg
     1981 catggggagg cgaccaactc catggcttct gataaccagc ctgaaggtat gatctcagag
     2041 tcacttgata acttggaatc catgatgccc aacaaggtca ggaagattgg cgagctcgtg
     2101 agatacctga acaccaaccc tgtgggtggc cttttggagt acgcccgctc ccatggcttt
     2161 gctgctgaat tcaagttggt cgaccagtcc ggacctcctc acgagcccaa gttcgtttac
     2221 caagcaaaag ttgggggtcg ctggttccca gccgtctgcg cacacagcaa gaagcaaggc
     2281 aagcaggaag cagcagatgc ggctctccgt gtcttgattg gggagaacga gaaggcagaa
     2341 cgcatgggtt tcacagaggt aaccccagtg acaggggcca gtctcagaag aactatgctc
     2401 ctcctctcaa ggtccccaga agcacagcca aagacactcc ctctcactgg cagcaccttc
     2461 catgaccaga tagccatgct gagccaccgg tgcttcaaca ctctgactaa cagcttccag
     2521 ccctccttgc tcggccgcaa gattctggcc gccatcatta tgaaaaaaga ctctgaggac
     2581 atgggtgtcg tcgtcagctt gggaacaggg aatcgctgtg taaaaggaga ttctctcagc
     2641 ctaaaaggag aaactgtcaa tgactgccat gcagaaataa tctcccggag aggcttcatc
     2701 aggtttctct acagtgagtt aatgaaatac aactcccaga ctgcgaagga tagtatattt
     2761 gaacctgcta agggaggaga aaagctccaa ataaaaaaga ctgtgtcatt ccatctgtat
     2821 atcagcactg ctccgtgtgg agatggcgcc ctctttgaca agtcctgcag cgaccgtgct
     2881 atggaaagca cagaatcccg ccactaccct gtcttcgaga atcccaaaca aggaaagctc
     2941 cgcaccaagg tggagaacgg agaaggcaca atccctgtgg aatccagtga cattgtgcct
     3001 acgtgggatg gcattcggct cggggagaga ctccgtacca tgtcctgtag tgacaaaatc
     3061 ctacgctgga acgtgctggg cctgcaaggg gcactgttga cccacttcct gcagcccatt
     3121 tatctcaaat ctgtcacatt gggttacctt ttcagccaag ggcatctgac ccgtgctatt
     3181 tgctgtcgtg tgacaagaga tgggagtgca tttgaggatg gactacgaca tccctttatt
     3241 gtcaaccacc ccaaggttgg cagagtcagc atatatgatt ccaaaaggca atccgggaag
     3301 actaaggaga caagcgtcaa ctggtgtctg gctgatggct atgacctgga gatcctggac
     3361 ggtaccagag gcactgtgga tgggccacgg aatgaattgt cccgggtctc caaaaagaac
     3421 atttttcttc tatttaagaa gctctgctcc ttccgttacc gcagggatct actgagactc
     3481 tcctatggtg aggccaagaa agctgcccgt gactacgaga cggccaagaa ctacttcaaa
     3541 aaaggcctga aggatatggg ctatgggaac tggattagca aaccccagga ggaaaagaac
     3601 ttttatctct gcccagtatt ggacccagct ttcttgtaca aagtggttga tatccagcac
     3661 agtggcggcc gctcgagtct agagggcccg cggttcgaaa ccagcggagg cggcggaagc
     3721 gggggaggag gcagcggcgg aggcggcagc gggggcggcg gaagcgctac cggtaagcct
     3781 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtatggcttc taactttact
     3841 cagttcgttc tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc
     3901 gctaacgggg tcgctgaatg gatcagctct aactcgcgat cacaggctta caaagtaacc
     3961 tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct
     4021 aaagtggcaa cccagactgt tggtggtgaa gagcttcctg tagccggatg gagatcttac
     4081 ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga gcttattgtt
     4141 aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcggccat cgcggccaac
     4201 tccggcatct acggaggtgg aggtggatct cgtacgggtt agtaatgagt ttaaacccgc
     4261 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4321 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4381 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4441 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4501 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4561 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4621 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4681 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4741 gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt
     4801 tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg
     4861 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     4921 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     4981 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5041 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     5101 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     5161 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     5221 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     5281 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     5341 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     5401 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     5461 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     5521 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     5581 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     5641 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     5701 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     5761 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     5821 aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat
     5881 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5941 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6001 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6061 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6121 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6301 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6361 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6961 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7621 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7681 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7741 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7801 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7861 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7921 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7981 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8041 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8101 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8161 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8281 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     8341 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8401 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8461 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     8521 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagg cttttgcaaa
     8581 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     8641 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     8701 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     8761 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     8821 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     8881 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     8941 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     9001 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     9061 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     9121 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     9181 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     9241 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     9301 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     9361 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     9421 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     9481 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     9541 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     9601 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     9661 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     9721 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     9781 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     9841 cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt
     9901 aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac
     9961 aagtttgtac aaaaaagcag gctccaccat gaatccgcgg caggggtatt ccctcagcgg
    10021 atactacacc catccatttc aaggctatga gcacagacag ctcagatacc agcagcctgg
    10081 gccaggatct tcccccagta gtttcctgct taagcaaata gaatttctca aggggcagct
    10141 cccagaagca ccggtgattg gaaagcagac accgtcactg ccaccttccc tcccaggact
    10201 ccggccaagg tttccagtac tacttgcctc cagtaccaga ggcaggcaag tggacatcag
    10261 gggtgtcccc aggggcgtgc atctcggaag tcaggggctc cagagagggt tccagcatcc
    10321 ttcaccacgt ggcaggagtc tgccacagag aggtgttgat tgcctttcct cacatttcca
    10381 ggaactgagt atctaccaag atcaggaaca aaggatctta aagttcctgg aagagcttgg
    10441 ggaagggaag gccaccacag cacatgatct gtctgggaaa cttgggactc cgaagaaaga
    10501 aatcaatcga gttttatact ccctggcaaa gaagggcaag ctacagaaag aggcaggaac
    10561 accccctttg tggaaaatcg cggtctccac tcaggcttgg aaccagcaca gcggagtggt
    10621 aagaccagac ggtcatagcc aagga
//
