LOCUS       sequence-402835-       15201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression vector of catalytically-dead dCas9-Set2 
            containing R195G and C201A mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15201)
  AUTHORS   Xu SJ, Lombroso SI, Fischer DK, Carpenter MD, Marchione DM, Hamilton
            PJ, Lim CJ, Neve RL, Garcia BA, Wimmer ME, Pierce RC, Heller EA
  TITLE     Chromatin-mediated alternative splicing regulates cocaine-reward 
            behavior.
  JOURNAL   Neuron. 2021 Sep 15;109(18):2943-2966.e8. doi: 
            10.1016/j.neuron.2021.08.008. Epub 2021 Sep 3.
  PUBMED    34480866
REFERENCE   2  (bases 1 to 15201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.08.008"; journalName: "Neuron"; date: 
            "2021-09-15- 15"; volume: "109"; issue: "18"; pages: "2943-2966.e8"
COMMENT     Sequence Label: hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry
FEATURES             Location/Qualifiers
     source          1..15201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        562..666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(667..735,736..1527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(885..904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1698..2286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2187..2206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2440..2457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2472..2612
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2538..2557)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2698..2720
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3968..4089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4223..4670
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    4776..4800
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4824..4889
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4896..4916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4926..4952,4953..4955,4956..7442,7443..7445,
                     7446..9029)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9084..9104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(11338..11362)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(11378..11953,11954..11956,11957..11965)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11546..11563)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11773..11792
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11966..12676
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(12111..12129)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     12379..12398
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    12819..13407
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12872..12892)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13290..13301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    13490..13611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    13931..14065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        15090..15194
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggggggggg tggggtgcag gggsrgggrg sggggggcgg gggggggcgg gggsacgsgg
       61 grgsgggggg rggrgsgggg ggrggrgsgg ggggrggrgs ggggggagga gsggggggrg
      121 gagcgggggg aggagcgggg ggaggagcgg ggggaggagc ggccagaccc cgaaaacggg
      181 ccccccccaa aacacacccc ccgggggtcg cgcgcggccc tttaaagcgg cggcggcggg
      241 cagcccgggc cccccgcggc cgagactagc gagttagaca ggcaagcact actcgcctct
      301 gcacgcacat gcttgcctgt caaactctac caccccggca cgctctctgt ctccatggcc
      361 cgccgccgcc gccatcgcgg cccccgccgc ccccggccgc ccgggcccac gggcgccgtc
      421 ccaaccgcac agtcccaggt aacgactcta ggaattaatt cttgaagacg aaagggcctc
      481 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      541 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      601 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      661 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      721 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      781 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      841 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      901 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      961 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1021 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1081 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1141 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1201 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1261 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1321 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1381 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1441 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1501 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     1861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2461 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2521 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     2581 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     2641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcatgaat
     2761 tattccatta tgcacgaccc cgccccgacg ccggcacgcc gggggcccgt ggccgcggcc
     2821 cgttggtcga acccccggcc ccgcccatcc gcgccatctg ccatgggcgg ggcgcgaggg
     2881 cgggtgggtc cgcgccccgc cccgcatggc atctcattac cgcccgatcc ggcggtttcc
     2941 gcttccgttc cgcatgctaa cgaggaacag gcagggggcg gggcccgggc cccgacttcc
     3001 cggttcggcg gtaatgagat acgagccccg cgcgcccgtt ggccgtcccc gggccccccg
     3061 gtcccgcccg ccggacgccg ggaccaacgg gacggcgggc ggcccaaggg nccgcccgcc
     3121 ttgccgcccc cccattggcc ggcgggcggg accgccccaa gggggcgggg ccgccgggta
     3181 aaagaagtga gaacgcgaag cgttcgcact tcgtcccaat atatatatat tattagggcg
     3241 aagtgcgagc actggcgccg tgcccgactc cgcgccggcc ccgggggcgg gcccgggcgg
     3301 cggggggcgg gtctctccgg cgcacataaa ggcccggcgc gaccgacgcc cgcagacggc
     3361 gccggccacg aacgacggga gcggctgcgg agcacgcgga ccgggagcgg gagtcgcaga
     3421 gggccgtcgg agcggacggc gtcggcatcg cgacgccccg gctcgggatc gggatcgcat
     3481 cggaaaggga cacgcggacg cgggggggaa agacccgccc accccaccca cgaaacacag
     3541 gggacgcacc ccgggggcct ccgacgacag aaacccaccg gtccgccttt tttgcacggg
     3601 taagcacctt gggtgggcgg aggagggggg gacgcggggg cggaggaggg gggacgcggg
     3661 ggcggnagga ggggggacgn cgggggcgga ggagggggga nncgcggggg cggaggaggg
     3721 gggacgcggg ggcggaggag ggggctcacc cgcgttcgtg ccttcccgca ggaggaacgt
     3781 cctcgtcgat aagcttgcat gcctgcaggt cgactctaga tgatcagatc ggtaccggat
     3841 ccgaattcga tatcctcgag caacgccgat ggcagcctgg cggggggtac cggatccgaa
     3901 ttcgatatcc tcgagctagg ctaggccttc tagagtcgac ccgggcggcc gcttcgagca
     3961 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4021 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4081 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     4141 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgattt aattaaagta
     4201 tctgcagagg gccctgcgta tgagtgcaag tgggttttag gaccaggatg aggcggggtg
     4261 ggggtgccta cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc
     4321 aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg
     4381 cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc
     4441 gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa actccccttc
     4501 ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc
     4561 gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc
     4621 ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag ctgtgctcct
     4681 gggcaccgcg cagtccgccc ccgcggctcc tggccagacc acccctagga ccccctgccc
     4741 caagtcgcag cctctagaag atctgtttaa acatcacaag tttgtacaaa aaagcaggct
     4801 ccgcggccgc ccccttcacc atggattata aagaccacga tggcgactac aaggaccacg
     4861 acatcgatta taaggacgac gacgacaaga tggcccccaa gaagaagaga aaggtcggaa
     4921 gaggcatgga caagaaatac tccattggcc tcgctatcgg cacaaactcc gtgggatggg
     4981 ccgtgattac cgatgaatac aaggtgccct ccaagaaatt caaagtgctc ggcaacacag
     5041 ataggcactc catcaagaag aacctgatcg gagccctgct ctttgattcc ggcgaaaccg
     5101 ccgaggctac caggctgaag agaacagcca gaaggagata cacaaggaga aagaatagga
     5161 tttgctatct ccaggagatc ttcagcaacg agatggccaa ggtggatgat agcttctttc
     5221 ataggctgga ggaaagcttc ctggtggaag aggacaagaa gcacgaaaga caccccattt
     5281 ttggcaatat tgtggacgag gtggcttatc acgagaagta tcctaccatt taccacctca
     5341 ggaagaagct ggtggatagc acagacaagg ctgatctgag actcatctac ctcgccctcg
     5401 ctcatatgat caaattcaga ggccacttcc tcatcgaagg cgacctcaac cccgacaact
     5461 ccgatgtgga taaactcttt atccagctcg tccaaaccta caaccagctc ttcgaggaaa
     5521 atcccatcaa cgctagcggc gtggatgcca aagctatcct cagcgccaga ctcagcaaat
     5581 ccaggaggct ggagaatctc atcgcccaac tgcctggaga gaagaagaat ggcctcttcg
     5641 gcaacctcat cgccctctcc ctcggcctca cacctaattt caagtccaat ttcgatctcg
     5701 ccgaagatgc taagctccag ctgtccaagg acacctacga cgacgacctc gacaacctgc
     5761 tcgcccaaat tggagatcag tacgccgacc tgtttctggc cgctaagaat ctgtccgacg
     5821 ccattctgct gagcgacatt ctgagagtca acacagagat tacaaaggct cccctcagcg
     5881 cttccatgat taaaaggtac gatgaacatc accaggatct caccctgctc aaggccctcg
     5941 tgaggcagca gctccctgaa aagtataaag agatcttttt cgaccagagc aagaatggct
     6001 acgccggata cattgacgga ggcgcttccc aggaggagtt ctacaaattc atcaagccca
     6061 tcctggaaaa gatggatggc accgaggaac tcctggtcaa actcaacagg gaagacctcc
     6121 tgagaaaaca gagaaccttc gataacggct ccattcccca tcagattcat ctgggcgagc
     6181 tgcacgctat cctcaggagg caagaggact tctacccctt cctgaaagac aatagggaaa
     6241 agatcgaaaa aatcctcaca ttcaggattc cttattacgt gggacctctg gccagaggca
     6301 atagcaggtt tgcctggatg accagaaaga gcgaggagac cattacccct tggaacttcg
     6361 aggaagtcgt ggataaaggc gcctccgccc agtccttcat tgagagaatg accaacttcg
     6421 ataaaaacct gcctaacgag aaggtgctcc ccaagcattc cctgctgtat gagtacttta
     6481 cagtgtacaa tgaactgaca aaagtgaaat atgtgaccga aggcatgagg aagcccgcct
     6541 ttctcagcgg agagcagaag aaagccatcg tggatctgct cttcaagaca aatagaaaag
     6601 tgaccgtcaa gcagctgaag gaagactact tcaagaaaat cgagtgtttc gactccgtgg
     6661 aaatctccgg cgtggaggac agatttaacg ccagcctggg cacctatcat gacctgctca
     6721 agatcatcaa agacaaagat ttcctcgaca acgaagagaa cgaggatatt ctggaggata
     6781 tcgtgctcac cctgacactc tttgaagaca gggagatgat cgaggagagg ctcaaaacct
     6841 acgcccatct gttcgacgac aaggtcatga agcaactgaa gaggaggagg tatacaggat
     6901 ggggcagact gagcaggaag ctgattaacg gcatcaggga taagcagtcc ggcaagacaa
     6961 tcctcgattt tctgaagagc gatggattcg ctaacagaaa tttcatgcag ctcattcacg
     7021 atgacagcct gaccttcaag gaggacattc agaaggctca ggtgtccggc caaggagaca
     7081 gcctccacga gcatatcgct aatctcgccg gatcccctgc cattaaaaaa ggaatcctcc
     7141 agaccgtgaa ggtggtcgat gagctcgtga aggtgatggg caggcacaaa cccgaaaaca
     7201 tcgtgatcga gatggctagg gagaatcaaa ccacccaaaa aggccagaag aacagcaggg
     7261 aaagaatgaa gaggatcgag gagggcatca aggagctggg aagccagatt ctgaaagaac
     7321 accctgtgga gaatacccag ctgcagaatg aaaagctgta cctctactac ctgcagaacg
     7381 gcagggatat gtacgtcgac caggagctcg acatcaatag gctgtccgac tatgacgtcg
     7441 acgccattgt cccccaaagc ttcctcaagg atgacagcat cgataacaag gtcctcacaa
     7501 ggtccgataa aaacagagga aagtccgata atgtgccctc cgaagaagtg gtgaaaaaaa
     7561 tgaaaaacta ctggagacag ctgctgaacg ccaagctgat tacacagaga aaattcgaca
     7621 atctgaccaa ggccgaaaga ggcggcctct ccgaactgga caaggccgga tttatcaaga
     7681 ggcagctcgt cgaaaccagg cagatcacaa aacacgtggc tcagatcctc gattccagga
     7741 tgaataccaa atatgacgaa aatgataaac tcatcaggga agtgaaggtg atcaccctca
     7801 aaagcaaact ggtcagcgac ttcaggaagg atttccagtt ctacaaagtg agagaaatca
     7861 acaactacca tcacgcccat gatgcctacc tcaacgccgt cgtcggcacc gccctcatca
     7921 agaagtatcc caaactggag tccgagtttg tctacggaga ttataaggtg tacgacgtga
     7981 ggaagatgat cgctaagagc gaacaggaaa tcggcaaggc cacagccaaa tacttttttt
     8041 acagcaatat tatgaacttc tttaagaccg agatcaccct ggccaatgga gaaattagga
     8101 agaggcccct gatcgagaca aacggcgaga ccggagagat cgtctgggat aagggcagag
     8161 attttgccac agtcaggaag gtcctctcca tgcctcaggt caacatcgtc aaaaagaccg
     8221 aagtccagac aggcggcttt agcaaggagt ccatcctgcc taagaggaac agcgacaaac
     8281 tcatcgctag aaaaaaggac tgggatccca agaaatacgg cggatttgac agccctacag
     8341 tcgcttattc cgtgctggtc gtcgctaaag tggagaaggg aaagtccaaa aagctcaaga
     8401 gcgtcaagga gctgctgggc atcaccatca tggaaagatc cagcttcgag aaaaacccta
     8461 tcgacttcct cgaagccaag ggctacaaag aagtgaagaa ggatctgatt atcaagctgc
     8521 ccaagtattc cctctttgag ctcgagaatg gaaggaagag gatgctcgcc agcgctggag
     8581 aactgcaaaa gggcaacgag ctggccctgc cttccaaata cgtgaacttt ctgtacctcg
     8641 cctcccacta tgagaaactc aagggaagcc ccgaggataa cgaacagaag cagctcttcg
     8701 tggaacagca caagcactat ctcgacgaga ttattgagca aattagcgag ttcagcaaga
     8761 gggtcatcct ggctgatgct aacctcgaca aagtgctgag cgcctacaat aagcacaggg
     8821 ataagcccat tagggagcag gccgagaata tcatccatct gttcaccctc acaaatctgg
     8881 gcgctcccgc cgctttcaag tacttcgaca ccacaattga taggaagaga tacaccagca
     8941 caaaggaggt gctggatgct accctcatcc accagagcat tacaggcctg tacgaaacca
     9001 gaattgacct gtcccaactc ggcggcgatt ccgctggcgg cggaggctcc ggcggcggcg
     9061 gctccggagg cggaggctcc ggacccaaga aaaagaggaa ggtcgctgcc gctggcagca
     9121 tgtccaagaa ccagagcgtg agcgccagcg aggatgagaa ggaaatcctc aacaacaacg
     9181 ccgagggcca taaacctcag agactgttcg atcaagagcc cgacctgacc gaggaagccc
     9241 tgaccaaatt cgaaaacctg gacgactgca tctacgccaa caagagaatc ggcacattca
     9301 agaataacga ttttatggaa tgcgactgct atgaagagtt ctccgacggc gtgaaccacg
     9361 cctgtgacga agactccgac tgcatcaaca gactcaccct gattgagtgt gtgaatgacc
     9421 tgtgcagctc ctgcggcaac gactgccaga accaaaggtt tcagaagaag cagtacgccc
     9481 ccatcgccat cttcaagacc aaacacaagg gctacggagt gagggctgaa caggacatcg
     9541 aggccaacca attcatttac gagtacaaag gcgaggtgat cgaggagatg gagtttaggg
     9601 ataggctcat tgactacgat cagaggcact ttaaacactt ctattttatg atgctccaga
     9661 acggagagtt cattgatgct acaatcaaag gaagcctggc tggcttttgt aaccacagcg
     9721 cctcccctaa cgcctacgtc aacaagtggg tggtcaagga caagctgaga atgggcatct
     9781 tcgctcagag gaaaatcctg aaaggcgagg agatcacatt cgactacaac gtggatagat
     9841 acggcgccca agcccagaag tgctactgcg aggaacccaa ttgtattggc ttcctgggag
     9901 gaaagaccca gaccgacgcc gccagcctcc tccctcagaa tatcgctgat gccctgggag
     9961 tgaccgtgtc catggagaag aagtggctca agctgaagaa gctgtccgga gagcccatca
    10021 tcaaaaatga aaacgaaaac attaacatcg aattcctgca gagcctggag gtgcagccca
    10081 tcgactcccc cgtggatgtg acaaagatca tgagcgtgct gctccagcag gacaacaaga
    10141 tcatcgccag caagctgctg aagaggctct tcacaatcga tgacgattcc ctgaggcatc
    10201 aggccatcaa actccatgga tacacctgtt ttagcaaaat gctcaagctg tttatcacag
    10261 agcagcccca ggtcgatggc aaaggcaatg agaccgagga agacgatatc aagttcatca
    10321 agggcattct cgacttcctg ctcgaactgc ccaaaaccac cagaaatggc atcgagagca
    10381 gccagatcga taatgtcgtg aagaccctgc ctgccaagtt cccctttctg aagcccaact
    10441 gcgacgagct gctggagaaa tggtccaagt ttgaaaccta caagaggatc acaaagaagg
    10501 acatcaacgt ggccgcctcc aagatgatcg acctcaggag ggtgaggctc cctcctggct
    10561 gggagatcat ccacgagaac ggcaggcccc tctactacaa tgccgagcag aagaccaaac
    10621 tgcactatcc tccctccggc agctccaagg tgttcagctc cagatccaac acccaggtca
    10681 actcccctag ctcctccgga atccccaaga cacccggcgc tctcgactcc aagaagcata
    10741 agctcagcga tgaggaatac gagaggaaga agcagaagag gctcgagtac gagaggatcg
    10801 ccctggaaag ggctaagcag gaagagctgg agagcctgaa gcagaagctg aaactcgaga
    10861 acgagagaaa gagcgtgctg gaggatatca ttgccgaagc caataagcag aaggagctgc
    10921 agaaggagga agctaagaag ctggtggaag ccaaggaagc caagagactc aagaggaaga
    10981 ccgtcagcca gagccagaga ctcgagcaca actggaataa atttttcgct agcttcgtcc
    11041 ccaacctgat caaaaagaac cctcagtcca agcagtttga ccacgaaaat atcaagcagt
    11101 gtgccaagga catcgtgaag atcctcacaa ccaaagagct gaaaaaggat agcagcagag
    11161 cccctcctga tgacctgacc aaaggaaaga ggcataaggt gaaggagttt atcaattcct
    11221 atatggacaa aatcattctc aaaaagaagc aaaaaaaggc cctcgctctc agcagcgcca
    11281 gcaccagaat gagcagcccc ccccctagca cctcctcctg aaagggtggg cgcgccgacc
    11341 cagctttctt gtacaaagtg gtttaaactg gtacgcgccc ctctcccgtc ccccccccct
    11401 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
    11461 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
    11521 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
    11581 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
    11641 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
    11701 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
    11761 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
    11821 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
    11881 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
    11941 acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggata acatggccat
    12001 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
    12061 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
    12121 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
    12181 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
    12241 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
    12301 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
    12361 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
    12421 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
    12481 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
    12541 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
    12601 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
    12661 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
    12721 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    12781 atgcaattgt ttcccgttat ttgcactctg ttcctgttaa tcaacctctg gattacaaaa
    12841 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    12901 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    12961 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    13021 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    13081 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    13141 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    13201 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
    13261 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    13321 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    13381 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggtc cgtgttgctt
    13441 ggtcttcacc tgtgcagact tgcgaaccat ggattcaatt gttgttgtta acttgtttat
    13501 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    13561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aagactagtc
    13621 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca
    13681 ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa
    13741 acttttggat gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc
    13801 cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat acgggtatta
    13861 tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg
    13921 acaaactcgg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    13981 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    14041 caatgtatct tatcatgtct ggatctctga cctgagattg gcggcactga ggtagagatg
    14101 cccgaacccc cccgagggag cgcgggacgc ggccggggag ggctggggcc ggggagggct
    14161 ggggccgggg agggctgggg ccggggaggg ctggggccgg ggagggctgg ggccggggag
    14221 ggctggggcy ggggagggsy ggggskgggg rggggncggg ggggggtgng ggnggggggg
    14281 gggtggggbg ggggngsggg kggkgggggg ggggggkggg ggggnngsgk gkgkkgkgkk
    14341 gkggggkggg ggggmggnng gbgbkgtggg nggngggggn nnnggggggg ggggngsngg
    14401 gsggscscsc gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    14461 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    14521 gggggggggs gggggggggc tggggctggg gnaggggcgg tggtgtgtag caggagcggt
    14581 gtgttgcgcc ggggtacgtc tggaggagcg ggaggtgcgc ggtgacgtgt ggatgaggaa
    14641 caggagttgt tgcgcggtga gttgtcgctg tgagttgtgt tgttgggcag gtgtggtgga
    14701 tgacgtgacg tgtgacgtgc ggagtgcgcc gtgctctgtt ggtttcacct gtggcagccc
    14761 gggccccccg cgggcgcgcg cgcgcgcaaa aaaggcgggc ggcggtccgg gcggcgtgcg
    14821 cgcgcgcggc gggcgtgggg ggcggggccg cgggagcggg gggaggagcg gggggaggag
    14881 sggcgggggg gcggcggggg gcgggggggg gtgggaggcc ggcgctggca ggctgcgacg
    14941 gccgtcgcgc aaccgcacag tcccaggtaa cgactctagg aattaattct tgaagacgaa
    15001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    15061 cgtcaggtgg yacttttcgg ggaaatgtgc gckgaacccc tatttgttta tttttctaaa
    15121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    15181 gaaaaaggaa gagtatgagy a
//
