LOCUS       sequence-402839-        8932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human FES kinase from a GAL promoter with a URA3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Hong SJ, Resnick SJ, Iketani S, Cha JW, Albert BA, Fazekas CT, Chang
            CW, Liu H, Dagan S, Abagyan MR, Fajtova P, Culbertson B, Brace B, 
            Reddem ER, Forouhar F, Glickman JF, Balkovec JM, Stockwell BR, 
            Shapiro L, O'Donoghue AJ, Sabo Y, Freundlich JS, Ho DD, Chavez A
  TITLE     A multiplex method for rapidly identifying viral protease 
            inhibitors.
  JOURNAL   Mol Syst Biol. 2025 Feb;21(2):158-172. doi: 
            10.1038/s44320-024-00082-1. Epub 2025 Jan 6.
  PUBMED    39762652
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44320-024-00082-1"; journalName: "Mol Syst Biol"; date: 
            "2025-02"; volume: "21"; issue: "2"; pages: "158-172"
COMMENT     Sequence Label: pAG426GAL-FES
FEATURES             Location/Qualifiers
     source          1..8932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1054..1495
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1054..1171
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1466..1489
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1510..1526
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1510..1526
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    1534..1558
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4034..4058)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4091..4107)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4092..4108)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      4110..4357
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4126..4144)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4375..4394)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4376..4394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4404..4421)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4404..4420)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4413..4435)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4561..5016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4648..4667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4858..4879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5147..5950)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5951..6166)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6190..6209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6309..6331
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6369..6387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      6428..7770
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6760..6807
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7797..7901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7902..7970,7971..8762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8120..8139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcta gtacggatta gaagccgccg agcgggtgac
     1081 agccctccga aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa
     1141 acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt
     1201 tatggttatg aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga
     1261 atcaaattaa caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta
     1321 attaatcagc gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca
     1381 taaccacttt aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta
     1441 ataaaagtat caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa
     1501 accccggatt ctagaactag tggatccccc atcacaagtt tgtacaaaaa agcaggctac
     1561 aaaatgggct tctcttccga gctgtgcagc ccccagggcc acggggtcct gcagcaaatg
     1621 caggaggccg agcttcgtct actggagggc atgagaaagt ggatggccca gcgggtcaag
     1681 agtgacaggg agtatgcagg actgcttcac cacatgtccc tgcaggacag tgggggccag
     1741 agccgggcca tcagccctga cagccccatc agtcagtcct gggctgagat caccagccaa
     1801 actgagggcc tgagccgctt gctgcggcag cacgcagagg atctgaactc agggcccctg
     1861 agcaagctga gcctgctcat ccgggaacgg cagcagcttc gcaagaccta cagcgagcag
     1921 tggcagcagc tgcagcagga gctcaccaag acccacagcc aggacattga gaagctgaag
     1981 agccagtacc gagctctggc acgggacagt gcccaagcca agcgcaagta ccaggaggcc
     2041 agcaaagaca aggaccgtga caaggccaag gacaagtatg tgcgcagcct gtggaagctc
     2101 tttgctcacc acaaccgcta tgtgctgggc gtgcgggctg cgcagctaca ccaccagcac
     2161 caccaccagc tcctgctgcc cggcctgctg cggtcactgc aggacctgca cgaggagatg
     2221 gcttgcatcc tgaaggagat cctgcaggaa tacctggaga ttagcagcct ggtgcaggat
     2281 gaggtggtgg ccattcaccg ggagatggct gcagctgctg cccgcatcca gcctgaggct
     2341 gagtaccaag gcttcctgcg acagtatggg tccgcacctg acgtcccacc ctgtgtcacg
     2401 ttcgatgagt cactgcttga ggagggtgaa ccgctggagc ctggggagct ccagctgaac
     2461 gagctgactg tggagagcgt gcagcacacg ctgacctcag tgacagatga gctggctgtg
     2521 gccaccgaga tggtgttcag gcggcaggag atggttacgc agctgcaaca ggagctccgg
     2581 aatgaagagg agaacaccca cccccgggag cgggtgcagc tgctgggcaa gaggcaagtg
     2641 ctgcaagaag cactgcaggg gctgcaggta gcgctgtgca gccaggccaa gctgcaggcc
     2701 cagcaggagt tgctgcagac caagctggag cacctgggcc ccggcgaacc cccgcctgtg
     2761 ctgctcctgc aggatgaccg ccactccacg tcgtcctcgg agcaggagcg agagggggga
     2821 aggacaccca cgctggagat ccttaagagc cacatctcag gaatcttccg ccccaagttc
     2881 tcgctccctc caccgctgca gctcattccg gaggtgcaga agcccctgca tgagcagctg
     2941 tggtaccacg gggccatccc gagggcagag gtggctgagc tgctggtgca ctctggggac
     3001 ttcctggtgc gggagagcca gggcaagcag gagtacgtgc tgtcggtgct gtgggatggt
     3061 ctgccccggc acttcatcat ccagtccttg gataacctgt accgactgga aggggaaggc
     3121 tttcctagca ttcctttgct catcgaccac ctactgagca cccagcagcc cctcaccaag
     3181 aagagtggtg ttgtcctgca cagggctgtg cccaaggaca agtgggtgct gaaccatgag
     3241 gacctggtgt tgggtgagca gattggacgg gggaactttg gcgaagtgtt cagcggacgc
     3301 ctgcgagccg acaacaccct ggtggcggtg aagtcttgtc gagagacgct cccacctgac
     3361 ctcaaggcca agtttctaca ggaagcgagg atcctgaagc agtacagcca ccccaacatc
     3421 gtgcgtctca ttggtgtctg cacccagaag cagcccatct acatcgtcat ggagcttgtg
     3481 caggggggcg acttcctgac cttcctccgc acggaggggg cccgcctgcg ggtgaagact
     3541 ctgctgcaga tggtggggga tgcagctgct ggcatggagt acctggagag caagtgctgc
     3601 atccaccggg acctggctgc tcggaactgc ctggtgacag agaagaatgt cctgaagatc
     3661 agtgactttg ggatgtcccg agaggaagcc gatggggtct atgcagcctc agggggcctc
     3721 agacaagtcc ccgtgaagtg gaccgcacct gaggccctta actacggccg ctactcctcc
     3781 gaaagcgacg tgtggagctt tggcatcttg ctctgggaga ccttcagcct gggggcctcc
     3841 ccctatccca acctcagcaa tcagcagaca cgggagtttg tggagaaggg gggccgtctg
     3901 ccctgcccag agctgtgtcc tgatgccgtg ttcaggctca tggagcagtg ctgggcctat
     3961 gagcctgggc agcggcccag cttcagcacc atctaccagg agctgcagag catccgaaag
     4021 cggcatcggt aaaacccagc tttcttgtac aaagtggtga tgggctgcag gaattcgata
     4081 tcaagcttat cgataccgtc gacctcgagt catgtaatta gttatgtcac gcttacattc
     4141 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
     4201 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
     4261 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
     4321 tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat
     4381 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4441 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4501 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4621 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4741 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4861 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4921 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4981 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     5041 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
     5101 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
     5161 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
     5221 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
     5281 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
     5341 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
     5401 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
     5461 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
     5521 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
     5581 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
     5641 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
     5701 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
     5761 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
     5821 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
     5881 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
     5941 ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga
     6001 atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc
     6061 tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag
     6121 gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagg tggtatggtg
     6181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     6241 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     6301 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     6361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     6421 ttagtatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
     6481 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
     6541 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
     6601 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
     6661 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     6721 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     6781 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
     6841 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
     6901 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
     6961 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
     7021 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
     7081 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
     7141 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
     7201 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
     7261 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
     7321 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
     7381 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
     7441 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
     7501 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
     7561 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
     7621 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
     7681 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
     7741 ttctacaaaa tgaagcacag atgcttcgtt caggtggcac ttttcgggga aatgtgcgcg
     7801 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7861 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7921 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7981 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8041 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8101 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8161 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8221 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8281 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8341 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8401 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8461 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8521 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8581 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8641 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8701 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8761 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8821 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8881 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
