LOCUS       sequence-402843-       10905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP
            indicator and soluble bPAC, a photoactivatable adenylyl cyclase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Xiang KM, Park P, Koren SA, Hayward RF, Cohen AE
  TITLE     All-optical mapping of cAMP transport reveals rules of sub-cellular 
            localization
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pLenti-CMV-Pink Flamindo-p2a-bPAC
FEATURES             Location/Qualifiers
     source          1..10905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3622..3641
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4388..4444
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5504..5533
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5534..5563
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    5597..6185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5650..5670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6068..6079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6188..6204)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6189..6205)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6710..6890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6916..6933)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6922..7146
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7192..7620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7279..7298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7489..7510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7629..7649)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7634..7963
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7814..7949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7876..7895
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8011..8058
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8077..8451
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8581..8702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8618..8637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8672..8691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8751..8767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8751..8767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8764..8786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8775..8791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8799..8805,8806..8823,8824..8829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8844..8865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8982..8999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9153..9741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9233..9252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9912..10703,10704..10772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10535..10554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10773..10877)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatgct agtgagcaag ggcgaggagg ataacatggc
     3241 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3301 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
     3361 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3421 gtacggctcc aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
     3481 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
     3541 cgttaaccag gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg
     3601 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggagcagtg
     3661 gcgcgatcct cctctcctgt cccagcgagg gcctgatgcc ctactcaccg tggcgctccg
     3721 gaagccccca ggtcagcgta cggatgaaga actggacctg atctttgaag agctgctgca
     3781 tatcaaggcg gtggcacacc tttctaactc ggtgaagcgg gaactagctg ctgttctgct
     3841 ctttgaacca cacagcaagg caggaactgt gttgttcagc cagggggaca agggtacctc
     3901 gtggtacatt atctggaagg gatctgtcaa tgtggtgacc catggcaagg ggctggtgac
     3961 cacgttgcac gagggagatg actttggaca gctggctctg gtgaacgacg cacctcgggc
     4021 agccaccatc atccttcgag aaaataactg tcactttctg cgtgtggaca agcaggactt
     4081 caaccgcatc atcaaggatg tggaatttaa agtggagact gttgatgagc ggatgtaccc
     4141 cgaggacggc gccctgaaga gcgagatcaa gaaggggctg aggctgaagg acggcggcca
     4201 ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4261 ctacatcgtc gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga
     4321 acagtgcgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     4381 ctccggagcc acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg
     4441 tcccatgaag cggctggtgt acatcagcaa gatcagcggc cacctgagcc tggaagagat
     4501 ccagcggatc ggcaaggtgt ccatcaagaa caaccagcgg gacaacatca ccggcgtgct
     4561 gctgtacctg cagggcctgt tcttccagat cctggaaggc gagaacgaga aggtggacaa
     4621 gctgtacaag aaaatcctgg tggacgaccg gcacaccaac atcctgtgcc tgaaaaccga
     4681 gtacgacatc accgaccgga tgttccccaa ctgggccatg aaaaccatca acctgaacga
     4741 gaacagcgag ctgatgatcc agcccatcaa gagcctgctg cagaccatca cccagagcca
     4801 ccgggtgctg gaaaagtaca tgcccgccag agtgatctac ctgatcaacc agggcatcaa
     4861 ccccctgacc gtggagcccc agctggtcga gaagatcatc ttcttcagcg acatcctggc
     4921 cttcagcacc ctgaccgaga agctgcccgt gaacgaggtg gtcatcctgg tcaaccggta
     4981 cttcagcatc tgcacccgga tcatcagcgc ctacggcggc gaagtgacca agttcatcgg
     5041 cgactgcgtg atggccagct tcaccaaaga gcagggcgac gccgccatcc ggaccagcct
     5101 ggacatcatc tccgagctga agcagctgcg gcaccacgtg gaggccacca accccctgca
     5161 cctgctgtac accggcatcg gcctgagcta cggccacgtg atcgagggca acatgggcag
     5221 cagcctgaag atggaccaca ccctgctggg ggacgccgtg aacgtggccg ccaggctgga
     5281 agccctgaca agacagctgc cctacgccct ggcctttaca gccggcgtga agaagtgctg
     5341 ccaggcccag tggaccttca tcaacctggg agcccaccag gtcaagggca agcaggaagc
     5401 catcgaggtc tacaccgtga atgaggccca gaagtactac gacaccctgc agatcaccca
     5461 gctgatccgg cagaccctgg aaaacgacaa gccgcggacg tacgagcaaa agctgatctc
     5521 ggaggaggac ctgcaccatc accatcacca tcaccatcac catctcgagt aagaattcga
     5581 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5641 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5701 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5761 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5821 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5881 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5941 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6001 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6061 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6121 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6181 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     6241 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6301 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6361 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6421 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     6481 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     6541 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     6601 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     6661 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     6721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     6901 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6961 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7021 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7081 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7141 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     7201 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7261 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7321 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7381 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7441 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7501 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7561 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7621 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7681 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7741 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7801 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7861 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7921 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7981 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     8041 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     8101 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     8161 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     8221 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     8281 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     8341 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     8401 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     8461 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8521 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8581 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8641 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8701 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8761 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8821 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8881 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8941 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9001 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9061 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9121 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9181 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9241 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9301 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9361 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9421 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9481 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9541 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9601 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9661 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9721 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9781 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9841 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9901 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9961 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10021 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10081 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10141 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10201 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10261 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10321 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10381 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10441 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10501 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10561 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10621 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10681 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10741 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10801 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10861 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
