LOCUS       sequence-402847-       11649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian co-expression of soluble Pink Flamindo and membrane-bound 
            bPAC for cAMP perturbation and imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Xiang KM, Park P, Koren SA, Hayward RF, Cohen AE
  TITLE     All-optical mapping of cAMP transport reveals rules of sub-cellular 
            localization
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX
FEATURES             Location/Qualifiers
     source          1..11649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3622..3641
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4388..4444
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4445..4447,4448..4450,4451..5161)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4490..4511)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4751..4770)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5098..5119
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6233..6262
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    6341..6929
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6394..6414)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6812..6823)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6932..6948)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6933..6949)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7454..7634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7660..7677)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7666..7890
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7936..8364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8023..8042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8233..8254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8373..8393)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8378..8707
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8558..8693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8620..8639
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8755..8802
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8821..9195
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9325..9446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9362..9381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9416..9435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9495..9511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9495..9511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9508..9530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9519..9535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9543..9549,9550..9567,9568..9573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9588..9609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9726..9743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9897..10485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9977..9996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10656..11447,11448..11516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11279..11298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11517..11621)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatgct agtgagcaag ggcgaggagg ataacatggc
     3241 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3301 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
     3361 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3421 gtacggctcc aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
     3481 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
     3541 cgttaaccag gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg
     3601 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggagcagtg
     3661 gcgcgatcct cctctcctgt cccagcgagg gcctgatgcc ctactcaccg tggcgctccg
     3721 gaagccccca ggtcagcgta cggatgaaga actggacctg atctttgaag agctgctgca
     3781 tatcaaggcg gtggcacacc tttctaactc ggtgaagcgg gaactagctg ctgttctgct
     3841 ctttgaacca cacagcaagg caggaactgt gttgttcagc cagggggaca agggtacctc
     3901 gtggtacatt atctggaagg gatctgtcaa tgtggtgacc catggcaagg ggctggtgac
     3961 cacgttgcac gagggagatg actttggaca gctggctctg gtgaacgacg cacctcgggc
     4021 agccaccatc atccttcgag aaaataactg tcactttctg cgtgtggaca agcaggactt
     4081 caaccgcatc atcaaggatg tggaatttaa agtggagact gttgatgagc ggatgtaccc
     4141 cgaggacggc gccctgaaga gcgagatcaa gaaggggctg aggctgaagg acggcggcca
     4201 ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4261 ctacatcgtc gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga
     4321 acagtgcgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     4381 ctccggagcc acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg
     4441 tcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4501 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4561 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4621 caccctcgtg accaccttca gctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4681 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4741 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4801 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4861 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4921 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4981 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5041 ccactacctg agccaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     5101 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5161 gggtggttct ggtatgaagc ggctggtgta catcagcaag atcagcggcc acctgagcct
     5221 ggaagagatc cagcggatcg gcaaggtgtc catcaagaac aaccagcggg acaacatcac
     5281 cggcgtgctg ctgtacctgc agggcctgtt cttccagatc ctggaaggcg agaacgagaa
     5341 ggtggacaag ctgtacaaga aaatcctggt ggacgaccgg cacaccaaca tcctgtgcct
     5401 gaaaaccgag tacgacatca ccgaccggat gttccccaac tgggccatga aaaccatcaa
     5461 cctgaacgag aacagcgagc tgatgatcca gcccatcaag agcctgctgc agaccatcac
     5521 ccagagccac cgggtgctgg aaaagtacat gcccgccaga gtgatctacc tgatcaacca
     5581 gggcatcaac cccctgaccg tggagcccca gctggtcgag aagatcatct tcttcagcga
     5641 catcctggcc ttcagcaccc tgaccgagaa gctgcccgtg aacgaggtgg tcatcctggt
     5701 caaccggtac ttcagcatct gcacccggat catcagcgcc tacggcggcg aagtgaccaa
     5761 gttcatcggc gactgcgtga tggccagctt caccaaagag cagggcgacg ccgccatccg
     5821 gaccagcctg gacatcatct ccgagctgaa gcagctgcgg caccacgtgg aggccaccaa
     5881 ccccctgcac ctgctgtaca ccggcatcgg cctgagctac ggccacgtga tcgagggcaa
     5941 catgggcagc agcctgaaga tggaccacac cctgctgggg gacgccgtga acgtggccgc
     6001 caggctggaa gccctgacaa gacagctgcc ctacgccctg gcctttacag ccggcgtgaa
     6061 gaagtgctgc caggcccagt ggaccttcat caacctggga gcccaccagg tcaagggcaa
     6121 gcaggaagcc atcgaggtct acaccgtgaa tgaggcccag aagtactacg acaccctgca
     6181 gatcacccag ctgatccggc agaccctgga aaacgacaag ccgcggacgt acgagcaaaa
     6241 gctgatctcg gaggaggacc tgaccggtgg taaaaagaag aaaaagaagt caaagacaaa
     6301 gtgtgtaatt atgtgagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     6361 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6421 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6481 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6541 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6601 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6661 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6721 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6781 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6841 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6901 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     6961 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7021 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7081 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7141 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7201 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     7261 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     7321 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     7381 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     7441 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7501 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7561 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7621 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     7681 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     7741 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7801 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7861 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7921 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7981 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8041 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8101 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8161 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8221 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8281 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8341 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     8401 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8461 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8521 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8581 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8641 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8701 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     8761 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     8821 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     8881 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     8941 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     9001 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     9061 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     9121 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     9181 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9241 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9301 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9421 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     9481 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10081 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10141 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10201 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10261 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10321 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10381 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10441 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10501 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11101 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11281 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11521 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11641 ccacctgac
//
