LOCUS       sequence-402853-       11679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian co-expression of membrane-bound Pink Flamindo and soluble 
            bPAC for cAMP perturbation and imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Xiang KM, Park P, Koren SA, Hayward RF, Cohen AE
  TITLE     All-optical mapping of cAMP transport reveals rules of sub-cellular 
            localization
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 11679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC
FEATURES             Location/Qualifiers
     source          1..11679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3670..3689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4436..4492
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4493..4495,4496..5206)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4493..4495,4496..5206)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4535..4556)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4796..4815)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5143..5164
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6278..6307
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6308..6337
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    6371..6959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6424..6444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6842..6853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6962..6978)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6963..6979)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7484..7664
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7690..7707)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7696..7920
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7966..8394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8053..8072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8263..8284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8403..8423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8408..8737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8588..8723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8650..8669
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8785..8832
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8851..9225
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9355..9476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9392..9411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9446..9465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9525..9541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9525..9541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9538..9560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9549..9565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9573..9579,9580..9597,9598..9603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9618..9639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9756..9773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9927..10515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10007..10026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10686..11477,11478..11546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11309..11328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11547..11651)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatggg ctgtgtgcag tgtaaggaca aggaggctac
     3241 caagctgaca ggaggcctgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3301 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3361 gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa
     3421 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3481 ggcctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg
     3541 cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga
     3601 ctcctccctg caggacggcg tattcatcta caaggtgaag ctgcgcggca ccaacttccc
     3661 ccccgacggc cccgtaatgc agaagaagac catgggctgg gagcagtggc gcgatcctcc
     3721 tctcctgtcc cagcgagggc ctgatgccct actcaccgtg gcgctccgga agcccccagg
     3781 tcagcgtacg gatgaagaac tggacctgat ctttgaagag ctgctgcata tcaaggcggt
     3841 ggcacacctt tctaactcgg tgaagcggga actagctgct gttctgctct ttgaaccaca
     3901 cagcaaggca ggaactgtgt tgttcagcca gggggacaag ggtacctcgt ggtacattat
     3961 ctggaaggga tctgtcaatg tggtgaccca tggcaagggg ctggtgacca cgttgcacga
     4021 gggagatgac tttggacagc tggctctggt gaacgacgca cctcgggcag ccaccatcat
     4081 ccttcgagaa aataactgtc actttctgcg tgtggacaag caggacttca accgcatcat
     4141 caaggatgtg gaatttaaag tggagactgt tgatgagcgg atgtaccccg aggacggcgc
     4201 cctgaagagc gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga
     4261 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga
     4321 catcaagttg gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg
     4381 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggct ccggagccac
     4441 gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc ccgtgagcaa
     4501 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4561 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4621 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4681 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4741 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4801 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4861 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4921 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4981 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5041 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5101 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5161 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggtg gttctggtat
     5221 gaagcggctg gtgtacatca gcaagatcag cggccacctg agcctggaag agatccagcg
     5281 gatcggcaag gtgtccatca agaacaacca gcgggacaac atcaccggcg tgctgctgta
     5341 cctgcagggc ctgttcttcc agatcctgga aggcgagaac gagaaggtgg acaagctgta
     5401 caagaaaatc ctggtggacg accggcacac caacatcctg tgcctgaaaa ccgagtacga
     5461 catcaccgac cggatgttcc ccaactgggc catgaaaacc atcaacctga acgagaacag
     5521 cgagctgatg atccagccca tcaagagcct gctgcagacc atcacccaga gccaccgggt
     5581 gctggaaaag tacatgcccg ccagagtgat ctacctgatc aaccagggca tcaaccccct
     5641 gaccgtggag ccccagctgg tcgagaagat catcttcttc agcgacatcc tggccttcag
     5701 caccctgacc gagaagctgc ccgtgaacga ggtggtcatc ctggtcaacc ggtacttcag
     5761 catctgcacc cggatcatca gcgcctacgg cggcgaagtg accaagttca tcggcgactg
     5821 cgtgatggcc agcttcacca aagagcaggg cgacgccgcc atccggacca gcctggacat
     5881 catctccgag ctgaagcagc tgcggcacca cgtggaggcc accaaccccc tgcacctgct
     5941 gtacaccggc atcggcctga gctacggcca cgtgatcgag ggcaacatgg gcagcagcct
     6001 gaagatggac cacaccctgc tgggggacgc cgtgaacgtg gccgccaggc tggaagccct
     6061 gacaagacag ctgccctacg ccctggcctt tacagccggc gtgaagaagt gctgccaggc
     6121 ccagtggacc ttcatcaacc tgggagccca ccaggtcaag ggcaagcagg aagccatcga
     6181 ggtctacacc gtgaatgagg cccagaagta ctacgacacc ctgcagatca cccagctgat
     6241 ccggcagacc ctggaaaacg acaagccgcg gacgtacgag caaaagctga tctcggagga
     6301 ggacctgcac catcaccatc accatcacca tcaccatctc gagtaagaat tcgatatcaa
     6361 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6421 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6481 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6541 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6601 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6661 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6721 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6781 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6841 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6901 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     6961 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7021 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7081 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7141 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     7201 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     7261 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7321 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     7381 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     7441 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     7501 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7561 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7621 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7681 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7741 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7801 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7861 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7921 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7981 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8041 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8101 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8161 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8221 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8281 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8341 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8641 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8761 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8821 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     8881 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     8941 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     9001 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     9061 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     9121 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     9181 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     9241 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     9301 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     9361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9481 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9541 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9601 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9661 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9721 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11641 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
