LOCUS       sequence-402860-       11694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian co-expression of membrane-bound Pink Flamindo and 
            membrane-bound bPAC for cAMP perturbation and imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Xiang KM, Park P, Koren SA, Hayward RF, Cohen AE
  TITLE     All-optical mapping of cAMP transport reveals rules of sub-cellular 
            localization
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX
FEATURES             Location/Qualifiers
     source          1..11694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3670..3689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4436..4492
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4493..4495,4496..5206)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4493..4495,4496..5206)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4535..4556)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4796..4815)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5143..5164
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6278..6307
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    6386..6974
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6439..6459)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6857..6868)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6977..6993)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6978..6994)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7499..7679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7705..7722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7711..7935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7981..8409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8068..8087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8278..8299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8418..8438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8423..8752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8603..8738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8665..8684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8800..8847
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8866..9240
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9370..9491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9407..9426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9461..9480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9540..9556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9540..9556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9553..9575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9564..9580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9588..9594,9595..9612,9613..9618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9633..9654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9771..9788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9942..10530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10022..10041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10701..11492,11493..11561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11324..11343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11562..11666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggack ttcctacttg gcagtacatc kacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagyattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatggg ctgtgtgcag tgtaaggaca aggaggctac
     3241 caagctgaca ggaggcctgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3301 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3361 gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa
     3421 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3481 ggcctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg
     3541 cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga
     3601 ctcctccctg caggacggcg tattcatcta caaggtgaag ctgcgcggca ccaacttccc
     3661 ccccgacggc cccgtaatgc agaagaagac catgggctgg gagcagtggc gcgatcctcc
     3721 tctcctgtcc cagcgagggc ctgatgccct actcaccgtg gcgctccgga agcccccagg
     3781 tcagcgtacg gatgaagaac tggacctgat ctttgaagag ctgctgcata tcaaggcggt
     3841 ggcacacctt tctaactcgg tgaagcggga actagctgct gttctgctct ttgaaccaca
     3901 cagcaaggca ggaactgtgt tgttcagcca gggggacaag ggtacctcgt ggtacattat
     3961 ctggaaggga tctgtcaatg tggtgaccca tggcaagggg ctggtgacca cgttgcacga
     4021 gggagatgac tttggacagc tggctctggt gaacgacgca cctcgggcag ccaccatcat
     4081 ccttcgagaa aataactgtc actttctgcg tgtggacaag caggacttca accgcatcat
     4141 caaggatgtg gaatttaaag tggagactgt tgatgagcgg atgtaccccg aggacggcgc
     4201 cctgaagagc gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga
     4261 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga
     4321 catcaagttg gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg
     4381 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggct ccggagccac
     4441 gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc ccgtgagcaa
     4501 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4561 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4621 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4681 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4741 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4801 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4861 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4921 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4981 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5041 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5101 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5161 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggtg gttctggtat
     5221 gaagcggctg gtgtacatca gcaagatcag cggccacctg agcctggaag agatccagcg
     5281 gatcggcaag gtgtccatca agaacaacca gcgggacaac atcaccggcg tgctgctgta
     5341 cctgcagggc ctgttcttcc agatcctgga aggcgagaac gagaaggtgg acaagctgta
     5401 caagaaaatc ctggtggacg accggcacac caacatcctg tgcctgaaaa ccgagtacga
     5461 catcaccgac cggatgttcc ccaactgggc catgaaaacc atcaacctga acgagaacag
     5521 cgagctgatg atccagccca tcaagagcct gctgcagacc atcacccaga gccaccgggt
     5581 gctggaaaag tacatgcccg ccagagtgat ctacctgatc aaccagggca tcaaccccct
     5641 gaccgtggag ccccagctgg tcgagaagat catcttcttc agcgacatcc tggccttcag
     5701 caccctgacc gagaagctgc ccgtgaacga ggtggtcatc ctggtcaacc ggtacttcag
     5761 catctgcacc cggatcatca gcgcctacgg cggcgaagtg accaagttca tcggcgactg
     5821 cgtgatggcc agcttcacca aagagcaggg cgacgccgcc atccggacca gcctggacat
     5881 catctccgag ctgaagcagc tgcggcacca cgtggaggcc accaaccccc tgcacctgct
     5941 gtacaccggc atcggcctga gctacggcca cgtgatcgag ggcaacatgg gcagcagcct
     6001 gaagatggac cacaccctgc tgggggacgc cgtgaacgtg gccgccaggc tggaagccct
     6061 gacaagacag ctgccctacg ccctggcctt tacagccggc gtgaagaagt gctgccaggc
     6121 ccagtggacc ttcatcaacc tgggagccca ccaggtcaag ggcaagcagg aagccatcga
     6181 ggtctacacc gtgaatgagg cccagaagta ctacgacacc ctgcagatca cccagctgat
     6241 ccggcagacc ctggaaaacg acaagccgcg gacgtacgag caaaagctga tctcggagga
     6301 ggacctgacc ggtggtaaaa agaagaaaaa gaagtcaaag acaaagtgtg taattatgtg
     6361 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     6421 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6481 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6541 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6601 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6661 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6721 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6781 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6841 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6901 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6961 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     7021 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7081 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     7141 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7201 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     7261 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     7321 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     7381 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     7441 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     7501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7681 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7741 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7801 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7861 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7921 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7981 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8041 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8101 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8161 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8221 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8281 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8341 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8401 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8761 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8821 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8881 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8941 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     9001 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     9061 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     9121 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     9181 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     9241 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10381 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
