LOCUS       sequence-402870-       10789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(DeltaR2-10::FlnAIg1-8)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-XT48-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..10789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6727..6736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6733..6735,6736..6738,6739..7452)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6778..6799)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7039..7058)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7386..7407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7574..7695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7611..7630)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7665..7684
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7702..8157)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7839..7860)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8051..8070
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8184..8288
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8290..8647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8290..8481
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8313..8333)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8498..8633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8682..9476
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8682..9476
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8736..8755)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9346..9365
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9664..9683)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9708..9755
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10084..10672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10573..10592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcaaagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccgc
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca catgccacct
     2101 ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca ggctgtgcag
     2161 gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca ggatgctgcc
     2221 tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca ctcccaggta
     2281 gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga tcctgcagag
     2341 acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct gacggagatg
     2401 tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa tggccggccc
     2461 cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg cagtgctcag
     2521 cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt gggccaggcc
     2581 agtggggagc tgttgcagca aattcccaaa ctgaacccga agaaagcccg tgcctacggg
     2641 ccaggcatcg agcccacagg caacatggtg aagaagcggg cagagttcac tgtggaaacc
     2701 agaagtgctg gccagggaga ggtgctggtg tacgtggagg acccggccgg acaccaggag
     2761 gaggcaaaag tgaccgccaa taacgacaag aaccgcacct tctccgtctg gtacgtcccc
     2821 gaggtgacgg ggactcataa ggttactgtg ctctttgctg gccagcacat cgccaagagc
     2881 cccttcgagg tgtacgtgga taagtcacag ggtgacgcca gcaaagtgac agcccaaggt
     2941 cccggcctgg agcccagtgg caacatcgcc aacaagacca cctactttga gatctttacg
     3001 gcaggagctg gcacgggcga ggtcgaggtt gtgatccagg accccatggg acagaagggc
     3061 acggtagagc ctcagctgga ggcccggggc gacagcacat accgctgcag ctaccagccc
     3121 accatggagg gcgtccacac cgtgcacgtc acgtttgccg gcgtgcccat ccctcgcagc
     3181 ccctacactg tcactgttgg ccaagcctgt aacccgagtg cctgccgggc ggttggccgg
     3241 ggcctccagc ccaagggtgt gcgggtgaag gagacagctg acttcaaggt gtacacaaag
     3301 ggcgctggca gtggggagct gaaggtcacc gtgaagggcc ccaagggaga ggagcgcgtg
     3361 aagcagaagg acctggggga tggcgtgtat ggcttcgagt attaccccat ggtccctgga
     3421 acctatatcg tcaccatcac gtggggtggt cagaacatcg ggcgcagtcc cttcgaagtg
     3481 aaggtgggca ccgagtgtgg caatcagaag gtacgggcct ggggccctgg gctggagggc
     3541 ggcgtcgttg gcaagtcagc agactttgtg gtggaggcta tcggggacga cgtgggcacg
     3601 ctgggcttct cggtggaagg gccatcgcag gctaagatcg aatgtgacga caagggcgac
     3661 ggctcctgtg atgtgcgcta ctggccgcag gaggctggcg agtatgccgt tcacgtgctg
     3721 tgcaacagcg aagacatccg cctcagcccc ttcatggctg acatccgtga cgcgccccag
     3781 gacttccacc cagacagggt gaaggcacgt gggcctggat tggagaagac aggtgtggcc
     3841 gtcaacaagc cagcagagtt cacagtggat gccaagcacg gtggcaaggc cccacttcgg
     3901 gtccaagtcc aggacaatga aggctgccct gtggaggcgt tggtcaagga caacggcaat
     3961 ggcacttaca gctgctccta cgtgcccagg aagccggtga agcacacagc catggtgtcc
     4021 tggggaggcg tcagcatccc caacagcccc ttcagggtga atgtgggagc tggcagccac
     4081 cccaacaagg tcaaagtata cggccccgga gtagccaaga cagggctcaa ggcccacgag
     4141 cccacctact tcactgtgga ctgcgccgag gctggccagg gggacgtcag catcggcatc
     4201 aagtgtgccc ctggagtggt aggccccgcc gaagctgaca tcgacttcga catcatccgc
     4261 aatgacaatg acaccttcac ggtcaagtac acgccccggg gggctggcag ctacaccatt
     4321 atggtcctct ttgctgacca ggccacgccc accagcccca tccgagtcaa ggtggagccc
     4381 tctcatgacg ccagtaaggt gaaggccgag ggccctggcc tcagtcgcac tggtgtcgag
     4441 cttggcaagc ccacccactt cacagtaaat gccaaagctg ctggcaaagg caagctggac
     4501 gtccagttct caggactcac caagggggat gcagtgcgag atgtggacat catcgaccac
     4561 catgacaaca cctacacagt caagtacacg cctgtccagc agggtccagt aggcgtcaat
     4621 gtcacttatg gaggggatcc catccctaag agccctttct cagtggcagt atctccaagc
     4681 ctggacctca gcaagatcaa ggtgtctggc ctgggagaga aggtggacgt tggcaaagac
     4741 caggagttca cagtcaaatc aaagggtgct ggtggtcaag gcaaagtggc atccaagatt
     4801 gtgggcccct cgggtgcagc ggtgccctgc aaggtggagc caggcctggg ggctgacaac
     4861 agtgtggtgc gcttcctgcc ccgtgaggaa gggccctatg aggtggaggt gacctatgac
     4921 ggcgtgcccg tgcctggcag cccctttcct ctggaagctg tgggtaccca ggcctgcatt
     4981 acagcagcca gtgctgtgtc tggtatcatt gctgacctcg acaccaccat catgttcgct
     5041 actgctggca cacttaaccg tgagggtgct gaaacttttg ctgaccaccg ggagggtatc
     5101 ttaaagacag caaaggttct tgtggaggac accaaggtcc tagtgcagaa tgcagctggg
     5161 agccaggaga agttggcaca agccgcccag tcctccgtgg ccaccattac ccgcctcgct
     5221 gatgtggtca agctcggtgc agccagccta ggagccgaag accctgaaac tcaggtggtg
     5281 ctgatcaatg cagtaaagga cgtagccaag gccctgggtg acctcatcag cgctacgaag
     5341 gctgcagcgg gcaaagttgg ggatgaccct gcagtgtggc agctcaagaa ctctgccaag
     5401 gtgatggtga ccaatgtgac atcattgctc aagacagtga aggctgtgga agatgaggcc
     5461 accaaaggca cacgggccct agaggcaacc acagagcaca tacgtcagga actggcggtc
     5521 ttctgttccc cagagccacc tgccaagacc tctacccctg aagacttcat ccgaatgacc
     5581 aagggtatta ctatggcaac agccaaagcc gttgctgctg gcaattcctg tcgacaggaa
     5641 gatgtcattg ccacagccaa tctgagccga cgggctattg cggacatgct tcgggcttgc
     5701 aaggaagcag ctttccaccc agaagtggcg cctgatgtac ggctccgagc cctgcactat
     5761 ggccgggagt gtgccaatgg ttacctggaa ctgctggacc acgtgctgct gacccttcag
     5821 aagccaaacc cagacctgaa gcagcagcta actggacact caaagcgggt tgctggctct
     5881 gtgactgagc tcatccaggc tgctgaagcc atgaaaggaa cagagtgggt ggacccagag
     5941 gaccctactg tcattgctga gaatgagctc ttgggagccg cagccgccat cgaggctgca
     6001 gccaagaagc tggagcagct gaagcccagg gccaaaccca aggaggcgga tgagtccttg
     6061 aactttgagg aacaaatcct agaagctgcc aagtccatcg ctgcagccac cagtgcactg
     6121 gtaaaggctg cgtcagcagc ccagagggag ctggtggctc aaggaaaggt gggcgccatt
     6181 ccagccaatg cactggatga tgggcagtgg tcgcagggcc tcatttccgc tgcccgtatg
     6241 gtggctgcgg ccaccaacaa tctgtgtgaa gcagctaacg cagctgtcca gggccatgct
     6301 agccaggaga aactcatttc ctcagccaag caagtagctg cctccacagc tcagctcctg
     6361 gtagcttgca aggtcaaagc agatcaggac tctgaggcaa tgaaacggct ccaggctgct
     6421 ggcaatgcag tgaagagggc ctcagataac ctggtaaagg cggcccagaa agctgcagcc
     6481 ttcgaagacc aggagaatga gacggtggtg gtgaaggaga aaatggttgg gggcattgcc
     6541 cagattatcg cagcacagga agagatgctt cggaaggaac gagagctgga agaggctcgg
     6601 aaaaagctcg cccagatccg gcagcagcag tacaagttct tgccttcaga gcttcgagac
     6661 gagcacggcg ctgggtccgg gtccgggcaa gggtccggcg caggctccgc gtcccgatca
     6721 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6781 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6841 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6901 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
     6961 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     7021 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     7081 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     7141 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     7201 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     7261 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     7321 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     7381 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7441 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     7501 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     7561 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7621 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7681 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7741 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7801 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7861 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7921 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7981 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     8041 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     8101 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     8161 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8221 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8281 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     8341 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8401 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8461 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8521 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8581 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8641 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     8701 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     8761 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     8821 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     8881 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8941 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     9001 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     9061 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     9121 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     9181 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     9241 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     9301 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     9361 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     9421 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     9481 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     9541 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9601 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     9661 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     9721 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     9781 ctggcactct gtcgataccc caccaagacc ccattggggc caatacgccc gcgtttcttc
     9841 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     9901 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     9961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10741 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattag
//
