LOCUS       sequence-402871-        8484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encodes the gene for murine neurogenin-2 under control of a 
            Tet-controlled promoter and the puromycin resistance gene under 
            control of a constitutive PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Mossink B, van Rhijn JR, Wang S, Linda K, Vitale MR, Zoller JEM, van
            Hugte EJH, Bak J, Verboven AHA, Selten M, Negwer M, Latour BL, van 
            der Werf I, Keller JM, Klein Gunnewiek TM, Schoenmaker C, Oudakker 
            A, Anania A, Jansen S, Lesch KP, Frega M, van Bokhoven H, Schubert 
            D, Nadif Kasri N
  TITLE     Cadherin-13 is a critical regulator of GABAergic modulation in human
            stem-cell-derived neuronal networks.
  JOURNAL   Mol Psychiatry. 2022 Jan;27(1):1-18. doi: 
            10.1038/s41380-021-01117-x. Epub 2021 May 10.
  PUBMED    33972691
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41380-021-01117-x"; journalName: "Mol Psychiatry"; date: 
            "2022-01"; volume: "27"; issue: "1"; pages: "1-18"
COMMENT     Sequence Label: pLV[TetOn]-Puro-TRE3G>mNeurog2
FEATURES             Location/Qualifiers
     source          1..8484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1964..1982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2000..2018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2072..2090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2243..2267
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2334..2358
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3157..3181)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3199..3787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3252..3272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3670..3681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3818..4317
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3853..3876)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4250..4269
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4334..4933
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4334..4353)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4830..4850
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5005..5238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5310..5431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5347..5366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5401..5420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5471..5606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5626..5645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5627..5645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5655..5672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5655..5671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5664..5686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5813..6268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5900..5919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6110..6131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6294..6398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6399..6467,6468..7259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6617..6636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7430..8018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7919..7938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8172..8189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8306..8327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8342..8347,8348..8365,8366..8372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8380..8396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8385..8407
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8404..8420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8404..8420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8439..8459
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8441..8459
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtt tactccctat cagtgataga
     1981 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     2041 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     2101 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     2161 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     2221 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     2281 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaacaagtt
     2341 tgtacaaaaa agcaggctgc caccatgttc gtcaaatctg agactctgga gttgaaggag
     2401 gaagaggagg tactgatgct gctgggctcg gcttccccgg cctcggcgac cctgaccccg
     2461 atgtcctcca gcgcggacga ggaggaggac gaggagctgc gccggccggg ctccgcgcgt
     2521 gggcagcgtg gagcggaagc cgggcagggg gtgcagggca gtccggcgtc gggtgccggg
     2581 ggttgccggc cagggcggct gctgggcctg atgcacgagt gcaagcgtcg cccgtcgcgc
     2641 tcacgggccg tctcccgagg tgccaagacg gcggagacgg tgcagcgcat caagaagacc
     2701 cgcaggctca aggccaacaa ccgcgagcgc aaccgcatgc acaacctaaa cgccgcgctg
     2761 gacgcgctgc gcgaggtgct gcccaccttc cccgaggatg ccaagctcac gaagatcgag
     2821 acgctgcgct tcgcccacaa ttacatctgg gcgctcaccg agactctgcg cctggcggac
     2881 cactgcgccg gcgccggtgg cctccagggg gcgctcttca cggaggcggt gctcctgagc
     2941 ccgggagctg cgctcggcgc cagcggggac agcccttctc caccttcctc ctggagctgc
     3001 accaacagcc cggcgtcatc ctccaactcc acgtccccat acagctgcac tttatcgccc
     3061 gctagccccg ggtcagacgt ggactactgg cagcccccac ctccggagaa gcatcgttat
     3121 gcgcctcacc tgcccctcgc cagggactgt atctagaccc agctttcttg tacaaagtgg
     3181 tgataatcga attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3601 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3781 tccccgcatc gggaattccc gcggttcgaa ttctaccggg taggggaggc gcttttccca
     3841 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     3901 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     3961 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     4021 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     4081 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     4141 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     4201 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     4261 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     4321 tcacgtggcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4381 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4441 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4501 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4561 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4621 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4681 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4741 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4801 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4861 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4921 gcccggtgcc tgaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4981 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5041 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5101 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5161 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5221 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5281 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5341 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5461 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5521 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5581 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5641 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5701 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5761 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5821 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5881 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5941 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6001 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6061 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6121 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6181 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6241 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6301 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6361 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6421 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6481 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6541 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6601 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6661 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6721 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6781 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6841 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6901 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6961 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7021 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7081 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7141 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7201 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7261 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7321 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7381 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7441 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7501 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7561 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7621 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7681 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7741 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7801 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaagag agaaaggcgg
     7861 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7921 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7981 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8041 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8101 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8161 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8221 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8281 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8341 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8401 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8461 aacaaaagct ggagctgcaa gctt
//
